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Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses

Shoot tip culture is a very effective approach for studying plant viruses. In this study, we evaluated the numbers, diversity, and titer of grapevine viruses in in vitro grapevine plants after long shoot tip culture. Six virus-infected grapevine cultivars (Cabernet Franc, Cabernet Gernischt, Caberne...

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Autores principales: Hu, Guojun, Dong, Yafeng, Zhang, Zunping, Fan, Xudong, Ren, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330417/
https://www.ncbi.nlm.nih.gov/pubmed/35893611
http://dx.doi.org/10.3390/plants11151907
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author Hu, Guojun
Dong, Yafeng
Zhang, Zunping
Fan, Xudong
Ren, Fang
author_facet Hu, Guojun
Dong, Yafeng
Zhang, Zunping
Fan, Xudong
Ren, Fang
author_sort Hu, Guojun
collection PubMed
description Shoot tip culture is a very effective approach for studying plant viruses. In this study, we evaluated the numbers, diversity, and titer of grapevine viruses in in vitro grapevine plants after long shoot tip culture. Six virus-infected grapevine cultivars (Cabernet Franc, Cabernet Gernischt, Cabernet Sauvignon, Wink, Victoria, and Merlot) collected from six regions of China were used as the research materials. Approximately 1.5 cm long shoot tips were used for meristem culture. The average survival rate of the six grapevine cultivars was 45.7%. Merlot collected from Beijing showed the highest survival rate (80.0%). Regeneration was not achieved in Cabernet Gernischt collected from Liaoning province and Cabernet Sauvignon from Tianjin due to bacterial and fungal contamination. Virus detection conducted in the surviving regenerated plants showed that the virus infection status, including the viral numbers and the species present in plants grown in vitro, was the same as that in corresponding in vivo plants. Moreover, the analysis of sequence diversity and the mutation frequency in grapevine viruses in vitro indicated that the structure of grapevine viruses was stable in long shoot tip culture after four sub-culture passages. Further, the relative viral titer of in vitro grapevine plants was much higher than that of in vivo plants. These results aid in the investigation of viruses in woody plants.
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spelling pubmed-93304172022-07-29 Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses Hu, Guojun Dong, Yafeng Zhang, Zunping Fan, Xudong Ren, Fang Plants (Basel) Communication Shoot tip culture is a very effective approach for studying plant viruses. In this study, we evaluated the numbers, diversity, and titer of grapevine viruses in in vitro grapevine plants after long shoot tip culture. Six virus-infected grapevine cultivars (Cabernet Franc, Cabernet Gernischt, Cabernet Sauvignon, Wink, Victoria, and Merlot) collected from six regions of China were used as the research materials. Approximately 1.5 cm long shoot tips were used for meristem culture. The average survival rate of the six grapevine cultivars was 45.7%. Merlot collected from Beijing showed the highest survival rate (80.0%). Regeneration was not achieved in Cabernet Gernischt collected from Liaoning province and Cabernet Sauvignon from Tianjin due to bacterial and fungal contamination. Virus detection conducted in the surviving regenerated plants showed that the virus infection status, including the viral numbers and the species present in plants grown in vitro, was the same as that in corresponding in vivo plants. Moreover, the analysis of sequence diversity and the mutation frequency in grapevine viruses in vitro indicated that the structure of grapevine viruses was stable in long shoot tip culture after four sub-culture passages. Further, the relative viral titer of in vitro grapevine plants was much higher than that of in vivo plants. These results aid in the investigation of viruses in woody plants. MDPI 2022-07-23 /pmc/articles/PMC9330417/ /pubmed/35893611 http://dx.doi.org/10.3390/plants11151907 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hu, Guojun
Dong, Yafeng
Zhang, Zunping
Fan, Xudong
Ren, Fang
Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
title Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
title_full Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
title_fullStr Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
title_full_unstemmed Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
title_short Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
title_sort effect of in vitro culture of long shoot tip on variant structure and titer of grapevine viruses
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330417/
https://www.ncbi.nlm.nih.gov/pubmed/35893611
http://dx.doi.org/10.3390/plants11151907
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