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A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury

We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) inju...

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Autores principales: He, Peng, Zhang, Mi, Zhao, Meng, Zhang, Mengyao, Ma, Benxu, Lv, Hongyu, Han, Yantao, Wu, Dingtao, Zhong, Zhangfeng, Zhao, Wenwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330552/
https://www.ncbi.nlm.nih.gov/pubmed/35911096
http://dx.doi.org/10.3389/fnut.2022.961182
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author He, Peng
Zhang, Mi
Zhao, Meng
Zhang, Mengyao
Ma, Benxu
Lv, Hongyu
Han, Yantao
Wu, Dingtao
Zhong, Zhangfeng
Zhao, Wenwen
author_facet He, Peng
Zhang, Mi
Zhao, Meng
Zhang, Mengyao
Ma, Benxu
Lv, Hongyu
Han, Yantao
Wu, Dingtao
Zhong, Zhangfeng
Zhao, Wenwen
author_sort He, Peng
collection PubMed
description We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe(2+) level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.
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spelling pubmed-93305522022-07-29 A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury He, Peng Zhang, Mi Zhao, Meng Zhang, Mengyao Ma, Benxu Lv, Hongyu Han, Yantao Wu, Dingtao Zhong, Zhangfeng Zhao, Wenwen Front Nutr Nutrition We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe(2+) level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis. Frontiers Media S.A. 2022-07-14 /pmc/articles/PMC9330552/ /pubmed/35911096 http://dx.doi.org/10.3389/fnut.2022.961182 Text en Copyright © 2022 He, Zhang, Zhao, Zhang, Ma, Lv, Han, Wu, Zhong and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
He, Peng
Zhang, Mi
Zhao, Meng
Zhang, Mengyao
Ma, Benxu
Lv, Hongyu
Han, Yantao
Wu, Dingtao
Zhong, Zhangfeng
Zhao, Wenwen
A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
title A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
title_full A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
title_fullStr A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
title_full_unstemmed A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
title_short A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
title_sort novel polysaccharide from chuanminshen violaceum and its protective effect against myocardial injury
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330552/
https://www.ncbi.nlm.nih.gov/pubmed/35911096
http://dx.doi.org/10.3389/fnut.2022.961182
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