Quantification of Canine Apocrine Gland Anal Sac Adenocarcinoma (AGASACA) Tumor Specimen Shrinkage after Formalin Fixation

SIMPLE SUMMARY: As the evidence in apocrine gland anal sac adenocarcinoma tumors grows, it is becoming more evident that stage-specific treatment strategies will become the mainstay. Thus, having confidence in the measurements of primary tumors is paramount. We aimed to quantify the degree of tumor tissue shrinkage after 24 and 48 h of formalin fixation to guide clinicians in their use of post-fixation measurements. We prospectively enrolled 23 client-owned dogs with naturally occurring apocrine gland anal sac adenocarcinoma that underwent surgical resection of at least the primary tumor. Measurements were recorded immediately before being placed in 10% buffered formalin and then again after 24 and 48 h of fixation, respectively. Overall, we found that tumors shrank by a mean of 4.8% and 7.2% after 24 and 48 h, respectively. This in real terms was a median of 1 mm. Other factors associated with the tumor, like the predominant microscopic pattern, the amount of necrosis, or the amount of fibrovascular stroma, did not have an impact on the degree of shrinkage. This study shows that the degree of shrinkage following formalin fixation should not impact the use of post-fixation measurements and can be used in clinical staging schema. ABSTRACT: The aim was to prospectively measure the shrinkage of primary apocrine gland anal sac adenocarcinoma (AGASACA) tumors after 24 and 48 h of formalin fixation. Dogs that were diagnosed with AGASACA pre-operatively by aspiration cytology were prospectively enrolled in the study. Tumor extirpation was performed in a closed technique. The tumor and associated tissues were examined on the back table away from the patient and the widest dimension of the tumor was measured using a sterile ruler (Medline(®); Northfield, IL, USA). This measurement was recorded in mm (t(0)). The tissue was placed in 10% buffered formalin and stored at room temperature. Two further measurements were taken after 24 (t(24)) and 48 (t(48)) hours of formalin fixation. Once the 48 h measurement was taken, the tissue was submitted for histopathology. The percentage of shrinkage between time points was calculated by using the following equation: (1 − [time b/time a]) × 100. Overall, 23 dogs with 23 tumors were enrolled. The mean percentage of shrinkage after 24 and 48 h of formalin fixation was 4.8% and 7.2%, respectively. The median diameter of the tumors reduced by 1 mm over 48 h and was not significantly different at any time point. These data will aid clinicians in interpreting measurements of AGASACA tumors following formalin fixation and shows that minimal change in tumor size is expected following 48 h.