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Influenza Viruses Suitable for Studies in Syrian Hamsters
Several small animal models, including mice, Syrian hamsters, guinea pigs, and ferrets are used to study the pathogenicity, transmissibility, and antigenicity of seasonal and pandemic influenza viruses. Moreover, animal models are essential for vaccination and challenge studies to evaluate the immun...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330595/ https://www.ncbi.nlm.nih.gov/pubmed/35893694 http://dx.doi.org/10.3390/v14081629 |
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author | Fan, Shufang Gu, Chunyang Kong, Huihui Guan, Lizheng Neumann, Gabriele Kawaoka, Yoshihiro |
author_facet | Fan, Shufang Gu, Chunyang Kong, Huihui Guan, Lizheng Neumann, Gabriele Kawaoka, Yoshihiro |
author_sort | Fan, Shufang |
collection | PubMed |
description | Several small animal models, including mice, Syrian hamsters, guinea pigs, and ferrets are used to study the pathogenicity, transmissibility, and antigenicity of seasonal and pandemic influenza viruses. Moreover, animal models are essential for vaccination and challenge studies to evaluate the immunogenicity and protective efficacy of new vaccines. However, authentic human influenza viruses do not always replicate efficiently in these animal models. Previously, we developed a high-yield A/Puerto Rico/8/34 (PR8-HY) vaccine virus backbone that conferred an increased virus yield to several seasonal influenza vaccines in eukaryotic cells and embryonated chicken eggs. Here, we show that this PR8-HY genetic backbone also increases the replication of several seasonal influenza viruses in Syrian hamsters compared to the authentic viruses. Therefore, the PR8-HY backbone is useful for animal studies to assess the biological properties of influenza viral HA and NA. |
format | Online Article Text |
id | pubmed-9330595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93305952022-07-29 Influenza Viruses Suitable for Studies in Syrian Hamsters Fan, Shufang Gu, Chunyang Kong, Huihui Guan, Lizheng Neumann, Gabriele Kawaoka, Yoshihiro Viruses Article Several small animal models, including mice, Syrian hamsters, guinea pigs, and ferrets are used to study the pathogenicity, transmissibility, and antigenicity of seasonal and pandemic influenza viruses. Moreover, animal models are essential for vaccination and challenge studies to evaluate the immunogenicity and protective efficacy of new vaccines. However, authentic human influenza viruses do not always replicate efficiently in these animal models. Previously, we developed a high-yield A/Puerto Rico/8/34 (PR8-HY) vaccine virus backbone that conferred an increased virus yield to several seasonal influenza vaccines in eukaryotic cells and embryonated chicken eggs. Here, we show that this PR8-HY genetic backbone also increases the replication of several seasonal influenza viruses in Syrian hamsters compared to the authentic viruses. Therefore, the PR8-HY backbone is useful for animal studies to assess the biological properties of influenza viral HA and NA. MDPI 2022-07-26 /pmc/articles/PMC9330595/ /pubmed/35893694 http://dx.doi.org/10.3390/v14081629 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fan, Shufang Gu, Chunyang Kong, Huihui Guan, Lizheng Neumann, Gabriele Kawaoka, Yoshihiro Influenza Viruses Suitable for Studies in Syrian Hamsters |
title | Influenza Viruses Suitable for Studies in Syrian Hamsters |
title_full | Influenza Viruses Suitable for Studies in Syrian Hamsters |
title_fullStr | Influenza Viruses Suitable for Studies in Syrian Hamsters |
title_full_unstemmed | Influenza Viruses Suitable for Studies in Syrian Hamsters |
title_short | Influenza Viruses Suitable for Studies in Syrian Hamsters |
title_sort | influenza viruses suitable for studies in syrian hamsters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330595/ https://www.ncbi.nlm.nih.gov/pubmed/35893694 http://dx.doi.org/10.3390/v14081629 |
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