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Influenza Viruses Suitable for Studies in Syrian Hamsters

Several small animal models, including mice, Syrian hamsters, guinea pigs, and ferrets are used to study the pathogenicity, transmissibility, and antigenicity of seasonal and pandemic influenza viruses. Moreover, animal models are essential for vaccination and challenge studies to evaluate the immun...

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Autores principales: Fan, Shufang, Gu, Chunyang, Kong, Huihui, Guan, Lizheng, Neumann, Gabriele, Kawaoka, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330595/
https://www.ncbi.nlm.nih.gov/pubmed/35893694
http://dx.doi.org/10.3390/v14081629
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author Fan, Shufang
Gu, Chunyang
Kong, Huihui
Guan, Lizheng
Neumann, Gabriele
Kawaoka, Yoshihiro
author_facet Fan, Shufang
Gu, Chunyang
Kong, Huihui
Guan, Lizheng
Neumann, Gabriele
Kawaoka, Yoshihiro
author_sort Fan, Shufang
collection PubMed
description Several small animal models, including mice, Syrian hamsters, guinea pigs, and ferrets are used to study the pathogenicity, transmissibility, and antigenicity of seasonal and pandemic influenza viruses. Moreover, animal models are essential for vaccination and challenge studies to evaluate the immunogenicity and protective efficacy of new vaccines. However, authentic human influenza viruses do not always replicate efficiently in these animal models. Previously, we developed a high-yield A/Puerto Rico/8/34 (PR8-HY) vaccine virus backbone that conferred an increased virus yield to several seasonal influenza vaccines in eukaryotic cells and embryonated chicken eggs. Here, we show that this PR8-HY genetic backbone also increases the replication of several seasonal influenza viruses in Syrian hamsters compared to the authentic viruses. Therefore, the PR8-HY backbone is useful for animal studies to assess the biological properties of influenza viral HA and NA.
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spelling pubmed-93305952022-07-29 Influenza Viruses Suitable for Studies in Syrian Hamsters Fan, Shufang Gu, Chunyang Kong, Huihui Guan, Lizheng Neumann, Gabriele Kawaoka, Yoshihiro Viruses Article Several small animal models, including mice, Syrian hamsters, guinea pigs, and ferrets are used to study the pathogenicity, transmissibility, and antigenicity of seasonal and pandemic influenza viruses. Moreover, animal models are essential for vaccination and challenge studies to evaluate the immunogenicity and protective efficacy of new vaccines. However, authentic human influenza viruses do not always replicate efficiently in these animal models. Previously, we developed a high-yield A/Puerto Rico/8/34 (PR8-HY) vaccine virus backbone that conferred an increased virus yield to several seasonal influenza vaccines in eukaryotic cells and embryonated chicken eggs. Here, we show that this PR8-HY genetic backbone also increases the replication of several seasonal influenza viruses in Syrian hamsters compared to the authentic viruses. Therefore, the PR8-HY backbone is useful for animal studies to assess the biological properties of influenza viral HA and NA. MDPI 2022-07-26 /pmc/articles/PMC9330595/ /pubmed/35893694 http://dx.doi.org/10.3390/v14081629 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fan, Shufang
Gu, Chunyang
Kong, Huihui
Guan, Lizheng
Neumann, Gabriele
Kawaoka, Yoshihiro
Influenza Viruses Suitable for Studies in Syrian Hamsters
title Influenza Viruses Suitable for Studies in Syrian Hamsters
title_full Influenza Viruses Suitable for Studies in Syrian Hamsters
title_fullStr Influenza Viruses Suitable for Studies in Syrian Hamsters
title_full_unstemmed Influenza Viruses Suitable for Studies in Syrian Hamsters
title_short Influenza Viruses Suitable for Studies in Syrian Hamsters
title_sort influenza viruses suitable for studies in syrian hamsters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330595/
https://www.ncbi.nlm.nih.gov/pubmed/35893694
http://dx.doi.org/10.3390/v14081629
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