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Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes

Periodontitis is a common inflammatory disease that is strongly influenced by dietary habits. Coffee is one of the most common dietary components; however, current research on the relationship between coffee consumption and periodontitis, as well as its underlying mechanisms, is limited. Based on a...

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Autores principales: Song, Jianan, Kim, Byunggook, Kim, Oksu, Yang, Ying, Liu, Danyang, Fu, Wenqi, Ma, Guowu, Kim, Young, Kim, Okjoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330743/
https://www.ncbi.nlm.nih.gov/pubmed/35892784
http://dx.doi.org/10.3390/foods11152199
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author Song, Jianan
Kim, Byunggook
Kim, Oksu
Yang, Ying
Liu, Danyang
Fu, Wenqi
Ma, Guowu
Kim, Young
Kim, Okjoon
author_facet Song, Jianan
Kim, Byunggook
Kim, Oksu
Yang, Ying
Liu, Danyang
Fu, Wenqi
Ma, Guowu
Kim, Young
Kim, Okjoon
author_sort Song, Jianan
collection PubMed
description Periodontitis is a common inflammatory disease that is strongly influenced by dietary habits. Coffee is one of the most common dietary components; however, current research on the relationship between coffee consumption and periodontitis, as well as its underlying mechanisms, is limited. Based on a previous report, caffeine (CA) and chlorogenic acid (CGA) were formulated into artificial coffee (AC) for this experiment. Cell viability, prostaglandin E(2) release, Western blotting, cellular reactive oxygen species (ROS) production, and NF-E2-related factor 2 (Nrf2) translocation analyses were performed to explore the effects of AC on lipopolysaccharide (LPS)-induced immortalized human oral keratinocytes (IHOKs) and elucidate their underlying mechanisms. AC pretreatment attenuated LPS-induced inflammatory mediator release, ROS production, and nuclear factor kappa B translocation in IHOKs. CA and CGA promoted AMP-activated protein kinase phosphorylation and down-regulated the nuclear factor-κB pathways to exert anti-inflammatory effects. Additionally, CGA promoted Nrf2 translocation and heme oxygenase-1 expression and showed anti-oxidative effects. Furthermore, AC, CA, and CGA components showed synergistic effects. Thus, we predict that coffee consumption may be beneficial for alleviating periodontitis. Moreover, the main coffee components CA and CGA seem to play a synergistic role in periodontitis.
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spelling pubmed-93307432022-07-29 Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes Song, Jianan Kim, Byunggook Kim, Oksu Yang, Ying Liu, Danyang Fu, Wenqi Ma, Guowu Kim, Young Kim, Okjoon Foods Article Periodontitis is a common inflammatory disease that is strongly influenced by dietary habits. Coffee is one of the most common dietary components; however, current research on the relationship between coffee consumption and periodontitis, as well as its underlying mechanisms, is limited. Based on a previous report, caffeine (CA) and chlorogenic acid (CGA) were formulated into artificial coffee (AC) for this experiment. Cell viability, prostaglandin E(2) release, Western blotting, cellular reactive oxygen species (ROS) production, and NF-E2-related factor 2 (Nrf2) translocation analyses were performed to explore the effects of AC on lipopolysaccharide (LPS)-induced immortalized human oral keratinocytes (IHOKs) and elucidate their underlying mechanisms. AC pretreatment attenuated LPS-induced inflammatory mediator release, ROS production, and nuclear factor kappa B translocation in IHOKs. CA and CGA promoted AMP-activated protein kinase phosphorylation and down-regulated the nuclear factor-κB pathways to exert anti-inflammatory effects. Additionally, CGA promoted Nrf2 translocation and heme oxygenase-1 expression and showed anti-oxidative effects. Furthermore, AC, CA, and CGA components showed synergistic effects. Thus, we predict that coffee consumption may be beneficial for alleviating periodontitis. Moreover, the main coffee components CA and CGA seem to play a synergistic role in periodontitis. MDPI 2022-07-24 /pmc/articles/PMC9330743/ /pubmed/35892784 http://dx.doi.org/10.3390/foods11152199 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Jianan
Kim, Byunggook
Kim, Oksu
Yang, Ying
Liu, Danyang
Fu, Wenqi
Ma, Guowu
Kim, Young
Kim, Okjoon
Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes
title Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes
title_full Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes
title_fullStr Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes
title_full_unstemmed Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes
title_short Effect of Coffee on Lipopolysaccharide-Induced Immortalized Human Oral Keratinocytes
title_sort effect of coffee on lipopolysaccharide-induced immortalized human oral keratinocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330743/
https://www.ncbi.nlm.nih.gov/pubmed/35892784
http://dx.doi.org/10.3390/foods11152199
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