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The Role of Nanoscale Distribution of Fibronectin in the Adhesion of Staphylococcus aureus Studied by Protein Patterning and DNA-PAINT
[Image: see text] Staphylococcus aureus is a widespread and highly virulent pathogen that can cause superficial and invasive infections. Interactions between S. aureus surface receptors and the extracellular matrix protein fibronectin mediate the bacterial invasion of host cells and is implicated in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330902/ https://www.ncbi.nlm.nih.gov/pubmed/35801826 http://dx.doi.org/10.1021/acsnano.2c00630 |
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author | Khateb, Heba Sørensen, Rasmus S. Cramer, Kimberly Eklund, Alexandra S. Kjems, Jorgen Meyer, Rikke L. Jungmann, Ralf Sutherland, Duncan S. |
author_facet | Khateb, Heba Sørensen, Rasmus S. Cramer, Kimberly Eklund, Alexandra S. Kjems, Jorgen Meyer, Rikke L. Jungmann, Ralf Sutherland, Duncan S. |
author_sort | Khateb, Heba |
collection | PubMed |
description | [Image: see text] Staphylococcus aureus is a widespread and highly virulent pathogen that can cause superficial and invasive infections. Interactions between S. aureus surface receptors and the extracellular matrix protein fibronectin mediate the bacterial invasion of host cells and is implicated in the colonization of medical implant surfaces. In this study, we investigate the role of distribution of both fibronectin and cellular receptors on the adhesion of S. aureus to interfaces as a model for primary adhesion at tissue interfaces or biomaterials. We present fibronectin in patches of systematically varied size (100–1000 nm) in a background of protein and bacteria rejecting chemistry based on PLL-g-PEG and studied S. aureus adhesion under flow. We developed a single molecule imaging assay for localizing fibronectin binding receptors on the surface of S. aureus via the super-resolution DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) technique. Our results indicate that S. aureus adhesion to fibronectin biointerfaces is regulated by the size of available ligand patterns, with an adhesion threshold of 300 nm and larger. DNA-PAINT was used to visualize fibronectin binding receptor organization in situ at ∼7 nm localization precision and with a surface density of 38–46 μm(–2), revealing that the engagement of two or more receptors is required for strong S. aureus adhesion to fibronectin biointerfaces. |
format | Online Article Text |
id | pubmed-9330902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93309022022-07-29 The Role of Nanoscale Distribution of Fibronectin in the Adhesion of Staphylococcus aureus Studied by Protein Patterning and DNA-PAINT Khateb, Heba Sørensen, Rasmus S. Cramer, Kimberly Eklund, Alexandra S. Kjems, Jorgen Meyer, Rikke L. Jungmann, Ralf Sutherland, Duncan S. ACS Nano [Image: see text] Staphylococcus aureus is a widespread and highly virulent pathogen that can cause superficial and invasive infections. Interactions between S. aureus surface receptors and the extracellular matrix protein fibronectin mediate the bacterial invasion of host cells and is implicated in the colonization of medical implant surfaces. In this study, we investigate the role of distribution of both fibronectin and cellular receptors on the adhesion of S. aureus to interfaces as a model for primary adhesion at tissue interfaces or biomaterials. We present fibronectin in patches of systematically varied size (100–1000 nm) in a background of protein and bacteria rejecting chemistry based on PLL-g-PEG and studied S. aureus adhesion under flow. We developed a single molecule imaging assay for localizing fibronectin binding receptors on the surface of S. aureus via the super-resolution DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) technique. Our results indicate that S. aureus adhesion to fibronectin biointerfaces is regulated by the size of available ligand patterns, with an adhesion threshold of 300 nm and larger. DNA-PAINT was used to visualize fibronectin binding receptor organization in situ at ∼7 nm localization precision and with a surface density of 38–46 μm(–2), revealing that the engagement of two or more receptors is required for strong S. aureus adhesion to fibronectin biointerfaces. American Chemical Society 2022-07-08 2022-07-26 /pmc/articles/PMC9330902/ /pubmed/35801826 http://dx.doi.org/10.1021/acsnano.2c00630 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Khateb, Heba Sørensen, Rasmus S. Cramer, Kimberly Eklund, Alexandra S. Kjems, Jorgen Meyer, Rikke L. Jungmann, Ralf Sutherland, Duncan S. The Role of Nanoscale Distribution of Fibronectin in the Adhesion of Staphylococcus aureus Studied by Protein Patterning and DNA-PAINT |
title | The Role of Nanoscale
Distribution of Fibronectin
in the Adhesion of Staphylococcus aureus Studied
by Protein Patterning and DNA-PAINT |
title_full | The Role of Nanoscale
Distribution of Fibronectin
in the Adhesion of Staphylococcus aureus Studied
by Protein Patterning and DNA-PAINT |
title_fullStr | The Role of Nanoscale
Distribution of Fibronectin
in the Adhesion of Staphylococcus aureus Studied
by Protein Patterning and DNA-PAINT |
title_full_unstemmed | The Role of Nanoscale
Distribution of Fibronectin
in the Adhesion of Staphylococcus aureus Studied
by Protein Patterning and DNA-PAINT |
title_short | The Role of Nanoscale
Distribution of Fibronectin
in the Adhesion of Staphylococcus aureus Studied
by Protein Patterning and DNA-PAINT |
title_sort | role of nanoscale
distribution of fibronectin
in the adhesion of staphylococcus aureus studied
by protein patterning and dna-paint |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330902/ https://www.ncbi.nlm.nih.gov/pubmed/35801826 http://dx.doi.org/10.1021/acsnano.2c00630 |
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