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Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo

The high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To furthe...

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Autores principales: Akhmedov, Shamil, Afanasyev, Sergey, Beshchasna, Natalia, Trusova, Marina, Stepanov, Ivan, Rebenkova, Mariya, Poletykina, Ekaterina, Vecherskiy, Yuri, Tverdokhlebov, Sergei, Bolbasov, Evgeny, Balakin, Sascha, Opitz, Joerg, Yermakov, Anatoly, Kozlov, Boris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331237/
https://www.ncbi.nlm.nih.gov/pubmed/35897812
http://dx.doi.org/10.3390/ijms23158241
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author Akhmedov, Shamil
Afanasyev, Sergey
Beshchasna, Natalia
Trusova, Marina
Stepanov, Ivan
Rebenkova, Mariya
Poletykina, Ekaterina
Vecherskiy, Yuri
Tverdokhlebov, Sergei
Bolbasov, Evgeny
Balakin, Sascha
Opitz, Joerg
Yermakov, Anatoly
Kozlov, Boris
author_facet Akhmedov, Shamil
Afanasyev, Sergey
Beshchasna, Natalia
Trusova, Marina
Stepanov, Ivan
Rebenkova, Mariya
Poletykina, Ekaterina
Vecherskiy, Yuri
Tverdokhlebov, Sergei
Bolbasov, Evgeny
Balakin, Sascha
Opitz, Joerg
Yermakov, Anatoly
Kozlov, Boris
author_sort Akhmedov, Shamil
collection PubMed
description The high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To further investigate these effects, the interaction of these nanoparticles with the adipose tissue of Wistar rats (in vivo) and human atherosclerotic plaques (ex vivo) was studied. For the in vivo study, cobalt–chromium (CoCr) alloy tubes, which are used for coronary stent manufacturing, were prepared with a coating of polylactic acid (PLA) which contained either modified or non-modified Fe@C NPs in a 5% by weight concentration. The tubes were implanted into an area of subcutaneous fat in Wistar rats, where changes in the histological structure and functional properties of the surrounding tissue were observed in the case of coatings modified with Fe@C NPs. For the ex vivo study, freshly explanted human atherosclerotic plaques were treated in the physiological solution with doses of modified Fe@C NPs, with mass equal to 5% or 25% relative to the plaques. This treatment resulted in the release of cholesterol-like compounds from the surface of the plaques into the solution, thus proving a pronounced destructive effect on the plaque structure. Chemically modified Fe@C NPs, when used as an anti-atherosclerosis agent, were able to activate the activity of macrophages, which could lead to the destruction of atherosclerotic plaques structures. These findings could prove the fabrication of next-generation vascular stents with built-in anti-atherosclerotic agents.
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spelling pubmed-93312372022-07-29 Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo Akhmedov, Shamil Afanasyev, Sergey Beshchasna, Natalia Trusova, Marina Stepanov, Ivan Rebenkova, Mariya Poletykina, Ekaterina Vecherskiy, Yuri Tverdokhlebov, Sergei Bolbasov, Evgeny Balakin, Sascha Opitz, Joerg Yermakov, Anatoly Kozlov, Boris Int J Mol Sci Article The high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To further investigate these effects, the interaction of these nanoparticles with the adipose tissue of Wistar rats (in vivo) and human atherosclerotic plaques (ex vivo) was studied. For the in vivo study, cobalt–chromium (CoCr) alloy tubes, which are used for coronary stent manufacturing, were prepared with a coating of polylactic acid (PLA) which contained either modified or non-modified Fe@C NPs in a 5% by weight concentration. The tubes were implanted into an area of subcutaneous fat in Wistar rats, where changes in the histological structure and functional properties of the surrounding tissue were observed in the case of coatings modified with Fe@C NPs. For the ex vivo study, freshly explanted human atherosclerotic plaques were treated in the physiological solution with doses of modified Fe@C NPs, with mass equal to 5% or 25% relative to the plaques. This treatment resulted in the release of cholesterol-like compounds from the surface of the plaques into the solution, thus proving a pronounced destructive effect on the plaque structure. Chemically modified Fe@C NPs, when used as an anti-atherosclerosis agent, were able to activate the activity of macrophages, which could lead to the destruction of atherosclerotic plaques structures. These findings could prove the fabrication of next-generation vascular stents with built-in anti-atherosclerotic agents. MDPI 2022-07-26 /pmc/articles/PMC9331237/ /pubmed/35897812 http://dx.doi.org/10.3390/ijms23158241 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akhmedov, Shamil
Afanasyev, Sergey
Beshchasna, Natalia
Trusova, Marina
Stepanov, Ivan
Rebenkova, Mariya
Poletykina, Ekaterina
Vecherskiy, Yuri
Tverdokhlebov, Sergei
Bolbasov, Evgeny
Balakin, Sascha
Opitz, Joerg
Yermakov, Anatoly
Kozlov, Boris
Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
title Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
title_full Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
title_fullStr Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
title_full_unstemmed Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
title_short Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
title_sort effect of chemically modified carbon-coated iron nanoparticles on the structure of human atherosclerotic plaques ex vivo and on adipose tissue in chronic experiment in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331237/
https://www.ncbi.nlm.nih.gov/pubmed/35897812
http://dx.doi.org/10.3390/ijms23158241
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