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Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions

The sharp increase in infections due to Staphylococcus aureus is associated with its ability to adapt to changes in its habitat. This study aimed to investigate the differences in the cytoplasmic amino acid profiles of a clinical strain of S. aureus under five combinations of stress-induced conditio...

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Autores principales: Alreshidi, Mousa, Dunstan, Hugh, Roberts, Tim, Bardakci, Fevzi, Badraoui, Riadh, Adnan, Mohd, Saeed, Mohd, Alreshidi, Fayez, Albulaihed, Yazeed, Snoussi, Mejdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331416/
https://www.ncbi.nlm.nih.gov/pubmed/35893561
http://dx.doi.org/10.3390/microorganisms10081503
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author Alreshidi, Mousa
Dunstan, Hugh
Roberts, Tim
Bardakci, Fevzi
Badraoui, Riadh
Adnan, Mohd
Saeed, Mohd
Alreshidi, Fayez
Albulaihed, Yazeed
Snoussi, Mejdi
author_facet Alreshidi, Mousa
Dunstan, Hugh
Roberts, Tim
Bardakci, Fevzi
Badraoui, Riadh
Adnan, Mohd
Saeed, Mohd
Alreshidi, Fayez
Albulaihed, Yazeed
Snoussi, Mejdi
author_sort Alreshidi, Mousa
collection PubMed
description The sharp increase in infections due to Staphylococcus aureus is associated with its ability to adapt to changes in its habitat. This study aimed to investigate the differences in the cytoplasmic amino acid profiles of a clinical strain of S. aureus under five combinations of stress-induced conditions representative of a wound site by varying temperature 35–37 °C, adding 0–5% NaCl and adjusting pH 6–8. The results indicated that aspartic acid, lysine, glutamic acid and histidine were the most abundant cytoplasmic amino acids in the control samples grown under optimal growth conditions. However, the magnitudes and levels of these amino acids were altered under the various wound site conditions, which led to differential cytoplasmic amino acid profiles as characterized by multivariate analyses (PLS-DA). The total cytoplasmic amino acid content was significantly reduced in the cells grown with 2.5% NaCl added at pH 7 and 37 °C relative to the control samples and other growth regimes. However, all combinations of enhanced stress conditions showed unique and characteristic changes in the concentration profiles of the cytoplasmic amino acids. These outcomes supported the hypothesis that bacterial cells of S. aureus maintain different metabolic homeostasis under various stress-induced conditions. The potent capability of S. aureus to constantly and rapidly acclimatize to variations within the environment may reflect the crucial feature supporting its virulence as an opportunistic pathogenic bacterium to invade the wound site. Understanding the control systems governing these marked changes in amino acids during the adaptation to the potential wound site conditions of this dangerous bacterium may offer new clinical controls to combat infection.
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spelling pubmed-93314162022-07-29 Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions Alreshidi, Mousa Dunstan, Hugh Roberts, Tim Bardakci, Fevzi Badraoui, Riadh Adnan, Mohd Saeed, Mohd Alreshidi, Fayez Albulaihed, Yazeed Snoussi, Mejdi Microorganisms Article The sharp increase in infections due to Staphylococcus aureus is associated with its ability to adapt to changes in its habitat. This study aimed to investigate the differences in the cytoplasmic amino acid profiles of a clinical strain of S. aureus under five combinations of stress-induced conditions representative of a wound site by varying temperature 35–37 °C, adding 0–5% NaCl and adjusting pH 6–8. The results indicated that aspartic acid, lysine, glutamic acid and histidine were the most abundant cytoplasmic amino acids in the control samples grown under optimal growth conditions. However, the magnitudes and levels of these amino acids were altered under the various wound site conditions, which led to differential cytoplasmic amino acid profiles as characterized by multivariate analyses (PLS-DA). The total cytoplasmic amino acid content was significantly reduced in the cells grown with 2.5% NaCl added at pH 7 and 37 °C relative to the control samples and other growth regimes. However, all combinations of enhanced stress conditions showed unique and characteristic changes in the concentration profiles of the cytoplasmic amino acids. These outcomes supported the hypothesis that bacterial cells of S. aureus maintain different metabolic homeostasis under various stress-induced conditions. The potent capability of S. aureus to constantly and rapidly acclimatize to variations within the environment may reflect the crucial feature supporting its virulence as an opportunistic pathogenic bacterium to invade the wound site. Understanding the control systems governing these marked changes in amino acids during the adaptation to the potential wound site conditions of this dangerous bacterium may offer new clinical controls to combat infection. MDPI 2022-07-25 /pmc/articles/PMC9331416/ /pubmed/35893561 http://dx.doi.org/10.3390/microorganisms10081503 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alreshidi, Mousa
Dunstan, Hugh
Roberts, Tim
Bardakci, Fevzi
Badraoui, Riadh
Adnan, Mohd
Saeed, Mohd
Alreshidi, Fayez
Albulaihed, Yazeed
Snoussi, Mejdi
Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions
title Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions
title_full Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions
title_fullStr Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions
title_full_unstemmed Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions
title_short Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions
title_sort changes in amino acid metabolism of staphylococcus aureus following growth to the stationary phase under adjusted growth conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331416/
https://www.ncbi.nlm.nih.gov/pubmed/35893561
http://dx.doi.org/10.3390/microorganisms10081503
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