Cargando…

Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland

(1) Background: Taenia crassiceps is a cosmopolitan tapeworm endemic to the northern hemisphere with an indirect lifecycle. Its definitive hosts are carnivores, and its intermediate hosts are rodents and rabbits. Nonhuman primates in zoos appear to be highly susceptible to T. crassiceps cysticercosi...

Descripción completa

Detalles Bibliográficos
Autores principales: Samorek-Pieróg, Małgorzata, Karamon, Jacek, Brzana, Adam, Sobieraj, Lesław, Włodarczyk, Mariusz, Sroka, Jacek, Bełcik, Aneta, Korpysa-Dzirba, Weronika, Cencek, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331665/
https://www.ncbi.nlm.nih.gov/pubmed/35894058
http://dx.doi.org/10.3390/pathogens11080835
_version_ 1784758456506384384
author Samorek-Pieróg, Małgorzata
Karamon, Jacek
Brzana, Adam
Sobieraj, Lesław
Włodarczyk, Mariusz
Sroka, Jacek
Bełcik, Aneta
Korpysa-Dzirba, Weronika
Cencek, Tomasz
author_facet Samorek-Pieróg, Małgorzata
Karamon, Jacek
Brzana, Adam
Sobieraj, Lesław
Włodarczyk, Mariusz
Sroka, Jacek
Bełcik, Aneta
Korpysa-Dzirba, Weronika
Cencek, Tomasz
author_sort Samorek-Pieróg, Małgorzata
collection PubMed
description (1) Background: Taenia crassiceps is a cosmopolitan tapeworm endemic to the northern hemisphere with an indirect lifecycle. Its definitive hosts are carnivores, and its intermediate hosts are rodents and rabbits. Nonhuman primates in zoos appear to be highly susceptible to T. crassiceps cysticercosis. The aim of this study was to confirm the presence and the molecular characterization of T. crassiceps cysts isolated from a captive ring-tailed lemur. (2) Methods: Surgery revealed multifocal, transparent saccules containing several thin-walled tapeworm cysticerci. In some of the metacestodes, single or multiple exogenous buds from daughter cysticerci were spotted. A molecular analysis was performed to confirm our morphological examinations, using two protocols to obtain the partial nad1 and cox1 genes of the Taenia sp. (3) Results: On the basis of morphological features and molecular analysis, the cysticerci were identified as T. crassiceps metacestodes, and products taken from the PCRs were sequenced. With respect to interpreting the sequencing results of the obtained amplicons, we compared them with data in the GenBank database, proving that, in this case, the causative agent was indeed T. crassiceps. (4) Conclusions: The received data can be used to supplement descriptions of this species. To the best of our knowledge, this is the first case of cysticercosis caused by T. crassiceps in a nonhuman primate in Poland.
format Online
Article
Text
id pubmed-9331665
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93316652022-07-29 Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland Samorek-Pieróg, Małgorzata Karamon, Jacek Brzana, Adam Sobieraj, Lesław Włodarczyk, Mariusz Sroka, Jacek Bełcik, Aneta Korpysa-Dzirba, Weronika Cencek, Tomasz Pathogens Case Report (1) Background: Taenia crassiceps is a cosmopolitan tapeworm endemic to the northern hemisphere with an indirect lifecycle. Its definitive hosts are carnivores, and its intermediate hosts are rodents and rabbits. Nonhuman primates in zoos appear to be highly susceptible to T. crassiceps cysticercosis. The aim of this study was to confirm the presence and the molecular characterization of T. crassiceps cysts isolated from a captive ring-tailed lemur. (2) Methods: Surgery revealed multifocal, transparent saccules containing several thin-walled tapeworm cysticerci. In some of the metacestodes, single or multiple exogenous buds from daughter cysticerci were spotted. A molecular analysis was performed to confirm our morphological examinations, using two protocols to obtain the partial nad1 and cox1 genes of the Taenia sp. (3) Results: On the basis of morphological features and molecular analysis, the cysticerci were identified as T. crassiceps metacestodes, and products taken from the PCRs were sequenced. With respect to interpreting the sequencing results of the obtained amplicons, we compared them with data in the GenBank database, proving that, in this case, the causative agent was indeed T. crassiceps. (4) Conclusions: The received data can be used to supplement descriptions of this species. To the best of our knowledge, this is the first case of cysticercosis caused by T. crassiceps in a nonhuman primate in Poland. MDPI 2022-07-26 /pmc/articles/PMC9331665/ /pubmed/35894058 http://dx.doi.org/10.3390/pathogens11080835 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Samorek-Pieróg, Małgorzata
Karamon, Jacek
Brzana, Adam
Sobieraj, Lesław
Włodarczyk, Mariusz
Sroka, Jacek
Bełcik, Aneta
Korpysa-Dzirba, Weronika
Cencek, Tomasz
Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland
title Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland
title_full Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland
title_fullStr Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland
title_full_unstemmed Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland
title_short Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland
title_sort molecular confirmation of taenia crassiceps cysticercosis in a captive ring-tailed lemur (lemur catta) in poland
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331665/
https://www.ncbi.nlm.nih.gov/pubmed/35894058
http://dx.doi.org/10.3390/pathogens11080835
work_keys_str_mv AT samorekpierogmałgorzata molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT karamonjacek molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT brzanaadam molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT sobierajlesław molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT włodarczykmariusz molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT srokajacek molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT bełcikaneta molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT korpysadzirbaweronika molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland
AT cencektomasz molecularconfirmationoftaeniacrassicepscysticercosisinacaptiveringtailedlemurlemurcattainpoland