Cargando…

Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy

Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Gu, Shengchen, Zang, Xupeng, Jiang, Lei, Gu, Ting, Meng, Fanming, Huang, Sixiu, Cai, Gengyuan, Li, Zicong, Wu, Zhenfang, Hong, Linjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331794/
https://www.ncbi.nlm.nih.gov/pubmed/35897733
http://dx.doi.org/10.3390/ijms23158157
_version_ 1784758488930451456
author Gu, Shengchen
Zang, Xupeng
Jiang, Lei
Gu, Ting
Meng, Fanming
Huang, Sixiu
Cai, Gengyuan
Li, Zicong
Wu, Zhenfang
Hong, Linjun
author_facet Gu, Shengchen
Zang, Xupeng
Jiang, Lei
Gu, Ting
Meng, Fanming
Huang, Sixiu
Cai, Gengyuan
Li, Zicong
Wu, Zhenfang
Hong, Linjun
author_sort Gu, Shengchen
collection PubMed
description Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation.
format Online
Article
Text
id pubmed-9331794
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93317942022-07-29 Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy Gu, Shengchen Zang, Xupeng Jiang, Lei Gu, Ting Meng, Fanming Huang, Sixiu Cai, Gengyuan Li, Zicong Wu, Zhenfang Hong, Linjun Int J Mol Sci Article Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation. MDPI 2022-07-24 /pmc/articles/PMC9331794/ /pubmed/35897733 http://dx.doi.org/10.3390/ijms23158157 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gu, Shengchen
Zang, Xupeng
Jiang, Lei
Gu, Ting
Meng, Fanming
Huang, Sixiu
Cai, Gengyuan
Li, Zicong
Wu, Zhenfang
Hong, Linjun
Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy
title Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy
title_full Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy
title_fullStr Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy
title_full_unstemmed Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy
title_short Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy
title_sort differential microrna expression in porcine endometrium related to spontaneous embryo loss during early pregnancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331794/
https://www.ncbi.nlm.nih.gov/pubmed/35897733
http://dx.doi.org/10.3390/ijms23158157
work_keys_str_mv AT gushengchen differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT zangxupeng differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT jianglei differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT guting differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT mengfanming differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT huangsixiu differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT caigengyuan differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT lizicong differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT wuzhenfang differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy
AT honglinjun differentialmicrornaexpressioninporcineendometriumrelatedtospontaneousembryolossduringearlypregnancy