Cargando…

Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis

(1) Background: Automated blood culture headspace analysis for the detection of volatile organic compounds of microbial origin (mVOC) could be a non-invasive method for bedside rapid pathogen identification. We investigated whether analyzing the gaseous headspace of blood culture (BC) bottles throug...

Descripción completa

Detalles Bibliográficos
Autores principales: Euler, Maximilian, Perl, Thorsten, Eickel, Isabell, Dudakova, Anna, Maguilla Rosado, Esther, Drees, Carolin, Vautz, Wolfgang, Wieditz, Johannes, Meissner, Konrad, Kunze-Szikszay, Nils
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331843/
https://www.ncbi.nlm.nih.gov/pubmed/35892382
http://dx.doi.org/10.3390/antibiotics11080992
_version_ 1784758500891557888
author Euler, Maximilian
Perl, Thorsten
Eickel, Isabell
Dudakova, Anna
Maguilla Rosado, Esther
Drees, Carolin
Vautz, Wolfgang
Wieditz, Johannes
Meissner, Konrad
Kunze-Szikszay, Nils
author_facet Euler, Maximilian
Perl, Thorsten
Eickel, Isabell
Dudakova, Anna
Maguilla Rosado, Esther
Drees, Carolin
Vautz, Wolfgang
Wieditz, Johannes
Meissner, Konrad
Kunze-Szikszay, Nils
author_sort Euler, Maximilian
collection PubMed
description (1) Background: Automated blood culture headspace analysis for the detection of volatile organic compounds of microbial origin (mVOC) could be a non-invasive method for bedside rapid pathogen identification. We investigated whether analyzing the gaseous headspace of blood culture (BC) bottles through gas chromatography-ion mobility spectrometry (GC-IMS) enables differentiation of infected and non-infected; (2) Methods: BC were gained out of a rabbit model, with sepsis induced by intravenous administration of E. coli (EC group; n = 6) and control group (n = 6) receiving sterile LB medium intravenously. After 10 h, a pair of blood cultures was obtained and incubated for 36 h. The headspace from aerobic and anaerobic BC was sampled every two hours using an autosampler and analyzed using a GC-IMS device. MALDI-TOF MS was performed to confirm or exclude microbial growth in BCs; (3) Results: Signal intensities (SI) of 113 mVOC peak regions were statistically analyzed. In 24 regions, the SI trends differed between the groups and were considered to be useful for differentiation. The principal component analysis showed differentiation between EC and control group after 6 h, with 62.2% of the data variance described by the principal components 1 and 2. Single peak regions, for example peak region P_15, show significant SI differences after 6 h in the anaerobic environment (p < 0.001) and after 8 h in the aerobic environment (p < 0.001); (4) Conclusions: The results are promising and warrant further evaluation in studies with an extended microbial panel and indications concerning its transferability to human samples.
format Online
Article
Text
id pubmed-9331843
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93318432022-07-29 Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis Euler, Maximilian Perl, Thorsten Eickel, Isabell Dudakova, Anna Maguilla Rosado, Esther Drees, Carolin Vautz, Wolfgang Wieditz, Johannes Meissner, Konrad Kunze-Szikszay, Nils Antibiotics (Basel) Article (1) Background: Automated blood culture headspace analysis for the detection of volatile organic compounds of microbial origin (mVOC) could be a non-invasive method for bedside rapid pathogen identification. We investigated whether analyzing the gaseous headspace of blood culture (BC) bottles through gas chromatography-ion mobility spectrometry (GC-IMS) enables differentiation of infected and non-infected; (2) Methods: BC were gained out of a rabbit model, with sepsis induced by intravenous administration of E. coli (EC group; n = 6) and control group (n = 6) receiving sterile LB medium intravenously. After 10 h, a pair of blood cultures was obtained and incubated for 36 h. The headspace from aerobic and anaerobic BC was sampled every two hours using an autosampler and analyzed using a GC-IMS device. MALDI-TOF MS was performed to confirm or exclude microbial growth in BCs; (3) Results: Signal intensities (SI) of 113 mVOC peak regions were statistically analyzed. In 24 regions, the SI trends differed between the groups and were considered to be useful for differentiation. The principal component analysis showed differentiation between EC and control group after 6 h, with 62.2% of the data variance described by the principal components 1 and 2. Single peak regions, for example peak region P_15, show significant SI differences after 6 h in the anaerobic environment (p < 0.001) and after 8 h in the aerobic environment (p < 0.001); (4) Conclusions: The results are promising and warrant further evaluation in studies with an extended microbial panel and indications concerning its transferability to human samples. MDPI 2022-07-23 /pmc/articles/PMC9331843/ /pubmed/35892382 http://dx.doi.org/10.3390/antibiotics11080992 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Euler, Maximilian
Perl, Thorsten
Eickel, Isabell
Dudakova, Anna
Maguilla Rosado, Esther
Drees, Carolin
Vautz, Wolfgang
Wieditz, Johannes
Meissner, Konrad
Kunze-Szikszay, Nils
Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
title Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
title_full Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
title_fullStr Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
title_full_unstemmed Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
title_short Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
title_sort blood culture headspace gas analysis enables early detection of escherichia coli bacteremia in an animal model of sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331843/
https://www.ncbi.nlm.nih.gov/pubmed/35892382
http://dx.doi.org/10.3390/antibiotics11080992
work_keys_str_mv AT eulermaximilian bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT perlthorsten bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT eickelisabell bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT dudakovaanna bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT maguillarosadoesther bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT dreescarolin bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT vautzwolfgang bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT wieditzjohannes bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT meissnerkonrad bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis
AT kunzeszikszaynils bloodcultureheadspacegasanalysisenablesearlydetectionofescherichiacolibacteremiainananimalmodelofsepsis