Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)

Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to dis...

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Autores principales: Romanov, Dmitry V., Karlov, Gennady I., Divashuk, Mikhail G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332022/
https://www.ncbi.nlm.nih.gov/pubmed/35893604
http://dx.doi.org/10.3390/plants11151900
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author Romanov, Dmitry V.
Karlov, Gennady I.
Divashuk, Mikhail G.
author_facet Romanov, Dmitry V.
Karlov, Gennady I.
Divashuk, Mikhail G.
author_sort Romanov, Dmitry V.
collection PubMed
description Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.
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spelling pubmed-93320222022-07-29 Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.) Romanov, Dmitry V. Karlov, Gennady I. Divashuk, Mikhail G. Plants (Basel) Communication Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time. MDPI 2022-07-22 /pmc/articles/PMC9332022/ /pubmed/35893604 http://dx.doi.org/10.3390/plants11151900 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Romanov, Dmitry V.
Karlov, Gennady I.
Divashuk, Mikhail G.
Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
title Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
title_full Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
title_fullStr Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
title_full_unstemmed Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
title_short Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
title_sort developing oligo probes for chromosomes identification in hemp (cannabis sativa l.)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332022/
https://www.ncbi.nlm.nih.gov/pubmed/35893604
http://dx.doi.org/10.3390/plants11151900
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