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Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells

Non-alcoholic fatty liver disease and type 2 diabetes are representing symptoms of metabolic syndrome, which is often accompanied with hepatic fat accumulation and insulin resistance. Since liver is the major site of glucose and lipid metabolism, this study aimed to understand the effects of SCAAs a...

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Autores principales: Wang, Shuang, Jung, Soohan, Ko, Kwang Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332103/
https://www.ncbi.nlm.nih.gov/pubmed/35893906
http://dx.doi.org/10.3390/nu14153050
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author Wang, Shuang
Jung, Soohan
Ko, Kwang Suk
author_facet Wang, Shuang
Jung, Soohan
Ko, Kwang Suk
author_sort Wang, Shuang
collection PubMed
description Non-alcoholic fatty liver disease and type 2 diabetes are representing symptoms of metabolic syndrome, which is often accompanied with hepatic fat accumulation and insulin resistance. Since liver is the major site of glucose and lipid metabolism, this study aimed to understand the effects of SCAAs and BCAAs supplementations on glucose and lipid metabolism in HepG2 cells. These cells were pretreated with SAMe, betaine, taurine, and BCAA for 24 h, followed by treatments of a high concentration of glucose (50 mM) or palmitic acid (PA, 0.5 mM) for 48 h to simulate high-glucose and high-fat environments. Pretreatment of BCAA and SCAAs inhibited the fat accumulation. At the transcriptional level, glucose and PA treatment led to significant increase of mRNA gluconeogenic enzyme. The mRNA expression level of GLUT2 was decreased by 20% in the SAMe-treated group and inhibited glucose synthesis by reducing the level of gluconeogenic enzyme. After SAMe or BCAA pretreatment, the mRNA expression of lipogenic enzymes was decreased. The PPAR-γ expression was increased after BCAA pretreatment, but SAMe not only downregulated the expression of PPAR-γ, but also inhibited the expression of ChREBP approximately 20% and SREBP-1c decreased by about 15%. Taken together, the effect of SAMe on glucose and lipid metabolism is significant especially on inhibiting hepatic lipogenesis and gluconeogenesis under the metabolic syndrome environment.
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spelling pubmed-93321032022-07-29 Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells Wang, Shuang Jung, Soohan Ko, Kwang Suk Nutrients Article Non-alcoholic fatty liver disease and type 2 diabetes are representing symptoms of metabolic syndrome, which is often accompanied with hepatic fat accumulation and insulin resistance. Since liver is the major site of glucose and lipid metabolism, this study aimed to understand the effects of SCAAs and BCAAs supplementations on glucose and lipid metabolism in HepG2 cells. These cells were pretreated with SAMe, betaine, taurine, and BCAA for 24 h, followed by treatments of a high concentration of glucose (50 mM) or palmitic acid (PA, 0.5 mM) for 48 h to simulate high-glucose and high-fat environments. Pretreatment of BCAA and SCAAs inhibited the fat accumulation. At the transcriptional level, glucose and PA treatment led to significant increase of mRNA gluconeogenic enzyme. The mRNA expression level of GLUT2 was decreased by 20% in the SAMe-treated group and inhibited glucose synthesis by reducing the level of gluconeogenic enzyme. After SAMe or BCAA pretreatment, the mRNA expression of lipogenic enzymes was decreased. The PPAR-γ expression was increased after BCAA pretreatment, but SAMe not only downregulated the expression of PPAR-γ, but also inhibited the expression of ChREBP approximately 20% and SREBP-1c decreased by about 15%. Taken together, the effect of SAMe on glucose and lipid metabolism is significant especially on inhibiting hepatic lipogenesis and gluconeogenesis under the metabolic syndrome environment. MDPI 2022-07-25 /pmc/articles/PMC9332103/ /pubmed/35893906 http://dx.doi.org/10.3390/nu14153050 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Shuang
Jung, Soohan
Ko, Kwang Suk
Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells
title Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells
title_full Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells
title_fullStr Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells
title_full_unstemmed Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells
title_short Effects of Amino Acids Supplementation on Lipid and Glucose Metabolism in HepG2 Cells
title_sort effects of amino acids supplementation on lipid and glucose metabolism in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332103/
https://www.ncbi.nlm.nih.gov/pubmed/35893906
http://dx.doi.org/10.3390/nu14153050
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