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Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways

Daphne odora, a blooming shrub, has been traditionally used for various medicinal purposes. However, information on its anti-melanogenic activity and dermal application is limited. In this study, the Daphne odora extract (DOE), with constituents including daphnetin, was used to investigate depigment...

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Autores principales: Eom, Young Sic, Jeong, Dongho, Ryu, A-Reum, Song, Keon-Hyoung, Im, Dai Sig, Lee, Mi-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332310/
https://www.ncbi.nlm.nih.gov/pubmed/35892714
http://dx.doi.org/10.3390/cimb44080228
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author Eom, Young Sic
Jeong, Dongho
Ryu, A-Reum
Song, Keon-Hyoung
Im, Dai Sig
Lee, Mi-Young
author_facet Eom, Young Sic
Jeong, Dongho
Ryu, A-Reum
Song, Keon-Hyoung
Im, Dai Sig
Lee, Mi-Young
author_sort Eom, Young Sic
collection PubMed
description Daphne odora, a blooming shrub, has been traditionally used for various medicinal purposes. However, information on its anti-melanogenic activity and dermal application is limited. In this study, the Daphne odora extract (DOE), with constituents including daphnetin, was used to investigate depigmenting activity and the underlying mechanism of Daphne odora. DOE inhibited in vitro and cellular tyrosinase activity in a dose-dependent manner, and reduced the α-MSH-induced melanin biosynthesis to a control level. The protein expressions of melanin synthesis-related enzymes were also significantly reduced by DOE. Moreover, DOE decreased the phosphorylation of cAMP-response element binding proteins (CREBs) induced by α-MSH in B16F10 cells, while it activated phosphorylated extra-cellular signal-regulated kinases (ERKs) and protein kinase B (AKT) expression. These results suggest that DOE might inhibit the melanogenesis signaling pathways by activating ERK- and AKT-signaling pathways to regulate the expression of CREB and MITF and its downstream pathways. Therefore, DOE could potentially be developed as a depigmenting agent.
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spelling pubmed-93323102022-07-29 Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways Eom, Young Sic Jeong, Dongho Ryu, A-Reum Song, Keon-Hyoung Im, Dai Sig Lee, Mi-Young Curr Issues Mol Biol Article Daphne odora, a blooming shrub, has been traditionally used for various medicinal purposes. However, information on its anti-melanogenic activity and dermal application is limited. In this study, the Daphne odora extract (DOE), with constituents including daphnetin, was used to investigate depigmenting activity and the underlying mechanism of Daphne odora. DOE inhibited in vitro and cellular tyrosinase activity in a dose-dependent manner, and reduced the α-MSH-induced melanin biosynthesis to a control level. The protein expressions of melanin synthesis-related enzymes were also significantly reduced by DOE. Moreover, DOE decreased the phosphorylation of cAMP-response element binding proteins (CREBs) induced by α-MSH in B16F10 cells, while it activated phosphorylated extra-cellular signal-regulated kinases (ERKs) and protein kinase B (AKT) expression. These results suggest that DOE might inhibit the melanogenesis signaling pathways by activating ERK- and AKT-signaling pathways to regulate the expression of CREB and MITF and its downstream pathways. Therefore, DOE could potentially be developed as a depigmenting agent. MDPI 2022-07-22 /pmc/articles/PMC9332310/ /pubmed/35892714 http://dx.doi.org/10.3390/cimb44080228 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eom, Young Sic
Jeong, Dongho
Ryu, A-Reum
Song, Keon-Hyoung
Im, Dai Sig
Lee, Mi-Young
Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways
title Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways
title_full Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways
title_fullStr Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways
title_full_unstemmed Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways
title_short Daphne odora Exerts Depigmenting Effects via Inhibiting CREB/MITF and Activating AKT/ERK-Signaling Pathways
title_sort daphne odora exerts depigmenting effects via inhibiting creb/mitf and activating akt/erk-signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332310/
https://www.ncbi.nlm.nih.gov/pubmed/35892714
http://dx.doi.org/10.3390/cimb44080228
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