Cargando…

The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats

Although oral ulcers represent one of the most frequent oral mucosal diseases, the available treatment is not sufficient to provide complete ulcer recovery without side-effects. Therefore, the aim of our study was to prepare a mucoadhesive oral gel based on Galium verum ethanol extract (GVL gel) and...

Descripción completa

Detalles Bibliográficos
Autores principales: Vuletic, Miona, Jakovljevic, Vladimir, Zivanovic, Suzana, Papic, Milos, Papic, Mirjana, Mladenovic, Rasa, Zivkovic, Vladimir, Srejovic, Ivan, Jeremic, Jovana, Andjic, Marijana, Kocovic, Aleksandar, Sretenovic, Jasmina, Mitrovic, Slobodanka, Božin, Biljana, Kladar, Nebojša, Bolevich, Sergey, Bradic, Jovana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332434/
https://www.ncbi.nlm.nih.gov/pubmed/35897855
http://dx.doi.org/10.3390/molecules27154680
_version_ 1784758646046982144
author Vuletic, Miona
Jakovljevic, Vladimir
Zivanovic, Suzana
Papic, Milos
Papic, Mirjana
Mladenovic, Rasa
Zivkovic, Vladimir
Srejovic, Ivan
Jeremic, Jovana
Andjic, Marijana
Kocovic, Aleksandar
Sretenovic, Jasmina
Mitrovic, Slobodanka
Božin, Biljana
Kladar, Nebojša
Bolevich, Sergey
Bradic, Jovana
author_facet Vuletic, Miona
Jakovljevic, Vladimir
Zivanovic, Suzana
Papic, Milos
Papic, Mirjana
Mladenovic, Rasa
Zivkovic, Vladimir
Srejovic, Ivan
Jeremic, Jovana
Andjic, Marijana
Kocovic, Aleksandar
Sretenovic, Jasmina
Mitrovic, Slobodanka
Božin, Biljana
Kladar, Nebojša
Bolevich, Sergey
Bradic, Jovana
author_sort Vuletic, Miona
collection PubMed
description Although oral ulcers represent one of the most frequent oral mucosal diseases, the available treatment is not sufficient to provide complete ulcer recovery without side-effects. Therefore, the aim of our study was to prepare a mucoadhesive oral gel based on Galium verum ethanol extract (GVL gel) and reveal its healing effects in the model of aphthous stomatitis in rats. Rats with oral ulcers were divided into the following groups: control (untreated), gel base (ulcer was treated with the gel base, three times per day for 10 days), and GVL gel group (the ulcer was treated with GVL gel in the same way as the gel base). Animals from each group were sacrificed on days 0, 3, 6, and 10 for collecting blood and ulcer tissue samples. Healing properties of oral gel were determined by clinical evaluation, as well as biochemical and histopathological examinations. Our findings suggest a significant decrease in the ulcer size in GVL gel group, with healing effects achieved through the alleviation of oxidative stress, reduction in COX-2 immunopositivity, and increase in collagen content in buccal tissue. Significant ulcer repairing potential of GVL gel highlights this oral mucoadhesive gel as a promising tool for prevention and treatment of RAS.
format Online
Article
Text
id pubmed-9332434
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93324342022-07-29 The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats Vuletic, Miona Jakovljevic, Vladimir Zivanovic, Suzana Papic, Milos Papic, Mirjana Mladenovic, Rasa Zivkovic, Vladimir Srejovic, Ivan Jeremic, Jovana Andjic, Marijana Kocovic, Aleksandar Sretenovic, Jasmina Mitrovic, Slobodanka Božin, Biljana Kladar, Nebojša Bolevich, Sergey Bradic, Jovana Molecules Article Although oral ulcers represent one of the most frequent oral mucosal diseases, the available treatment is not sufficient to provide complete ulcer recovery without side-effects. Therefore, the aim of our study was to prepare a mucoadhesive oral gel based on Galium verum ethanol extract (GVL gel) and reveal its healing effects in the model of aphthous stomatitis in rats. Rats with oral ulcers were divided into the following groups: control (untreated), gel base (ulcer was treated with the gel base, three times per day for 10 days), and GVL gel group (the ulcer was treated with GVL gel in the same way as the gel base). Animals from each group were sacrificed on days 0, 3, 6, and 10 for collecting blood and ulcer tissue samples. Healing properties of oral gel were determined by clinical evaluation, as well as biochemical and histopathological examinations. Our findings suggest a significant decrease in the ulcer size in GVL gel group, with healing effects achieved through the alleviation of oxidative stress, reduction in COX-2 immunopositivity, and increase in collagen content in buccal tissue. Significant ulcer repairing potential of GVL gel highlights this oral mucoadhesive gel as a promising tool for prevention and treatment of RAS. MDPI 2022-07-22 /pmc/articles/PMC9332434/ /pubmed/35897855 http://dx.doi.org/10.3390/molecules27154680 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vuletic, Miona
Jakovljevic, Vladimir
Zivanovic, Suzana
Papic, Milos
Papic, Mirjana
Mladenovic, Rasa
Zivkovic, Vladimir
Srejovic, Ivan
Jeremic, Jovana
Andjic, Marijana
Kocovic, Aleksandar
Sretenovic, Jasmina
Mitrovic, Slobodanka
Božin, Biljana
Kladar, Nebojša
Bolevich, Sergey
Bradic, Jovana
The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats
title The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats
title_full The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats
title_fullStr The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats
title_full_unstemmed The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats
title_short The Evaluation of Healing Properties of Galium verum-Based Oral Gel in Aphthous Stomatitis in Rats
title_sort evaluation of healing properties of galium verum-based oral gel in aphthous stomatitis in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332434/
https://www.ncbi.nlm.nih.gov/pubmed/35897855
http://dx.doi.org/10.3390/molecules27154680
work_keys_str_mv AT vuleticmiona theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT jakovljevicvladimir theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT zivanovicsuzana theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT papicmilos theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT papicmirjana theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT mladenovicrasa theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT zivkovicvladimir theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT srejovicivan theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT jeremicjovana theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT andjicmarijana theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT kocovicaleksandar theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT sretenovicjasmina theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT mitrovicslobodanka theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT bozinbiljana theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT kladarnebojsa theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT bolevichsergey theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT bradicjovana theevaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT vuleticmiona evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT jakovljevicvladimir evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT zivanovicsuzana evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT papicmilos evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT papicmirjana evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT mladenovicrasa evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT zivkovicvladimir evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT srejovicivan evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT jeremicjovana evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT andjicmarijana evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT kocovicaleksandar evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT sretenovicjasmina evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT mitrovicslobodanka evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT bozinbiljana evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT kladarnebojsa evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT bolevichsergey evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats
AT bradicjovana evaluationofhealingpropertiesofgaliumverumbasedoralgelinaphthousstomatitisinrats