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Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences

The white-backed planthopper (WBPH), Sogatella furcifera, is one of the most important piercing-sucking pests of rice (Oryza sativa) in Asia. Mucin-like salivary protein (SFMLP) is highly expressed in the salivary glands of WBPH, which plays an important role in WBPH feeding. In this study, WBPH inj...

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Autores principales: Liu, Yudi, Yi, Jinyu, Jia, Haokang, Miao, Yutong, Hou, Maolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332473/
https://www.ncbi.nlm.nih.gov/pubmed/35897828
http://dx.doi.org/10.3390/ijms23158239
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author Liu, Yudi
Yi, Jinyu
Jia, Haokang
Miao, Yutong
Hou, Maolin
author_facet Liu, Yudi
Yi, Jinyu
Jia, Haokang
Miao, Yutong
Hou, Maolin
author_sort Liu, Yudi
collection PubMed
description The white-backed planthopper (WBPH), Sogatella furcifera, is one of the most important piercing-sucking pests of rice (Oryza sativa) in Asia. Mucin-like salivary protein (SFMLP) is highly expressed in the salivary glands of WBPH, which plays an important role in WBPH feeding. In this study, WBPH injected with dsSFMLP had difficulty in sucking phloem sap from rice plants, which significantly reduced their food intake, weight, and survival. In contrast, the knockdown of the SFMLP gene had only a marginal effect on the survival of WBPH fed an artificial diet. Further studies showed that silencing SFMLP resulted in the short and single-branched salivary sheaths secretion and less formation of salivary flanges in rice. These data suggest that SFMLP is involved in the formation of the salivary sheath and is essential for feeding in WBPH. Overexpression of the SFMLP gene in rice plants promoted the feeding of WBPH, whereas silencing the gene in rice plants significantly decreased WBPH performance. Additionally, it was found that overexpression of SFMLP in rice plants elicited the signalling pathway of SA (salicylic acid) while suppressing JA (jasmonic acid); in contrast, silencing of the SFMLP gene in rice plants showed the opposite results. This study clarified the function of SFMLP in WBPH feeding as well as mediating rice defences.
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spelling pubmed-93324732022-07-29 Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences Liu, Yudi Yi, Jinyu Jia, Haokang Miao, Yutong Hou, Maolin Int J Mol Sci Article The white-backed planthopper (WBPH), Sogatella furcifera, is one of the most important piercing-sucking pests of rice (Oryza sativa) in Asia. Mucin-like salivary protein (SFMLP) is highly expressed in the salivary glands of WBPH, which plays an important role in WBPH feeding. In this study, WBPH injected with dsSFMLP had difficulty in sucking phloem sap from rice plants, which significantly reduced their food intake, weight, and survival. In contrast, the knockdown of the SFMLP gene had only a marginal effect on the survival of WBPH fed an artificial diet. Further studies showed that silencing SFMLP resulted in the short and single-branched salivary sheaths secretion and less formation of salivary flanges in rice. These data suggest that SFMLP is involved in the formation of the salivary sheath and is essential for feeding in WBPH. Overexpression of the SFMLP gene in rice plants promoted the feeding of WBPH, whereas silencing the gene in rice plants significantly decreased WBPH performance. Additionally, it was found that overexpression of SFMLP in rice plants elicited the signalling pathway of SA (salicylic acid) while suppressing JA (jasmonic acid); in contrast, silencing of the SFMLP gene in rice plants showed the opposite results. This study clarified the function of SFMLP in WBPH feeding as well as mediating rice defences. MDPI 2022-07-26 /pmc/articles/PMC9332473/ /pubmed/35897828 http://dx.doi.org/10.3390/ijms23158239 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yudi
Yi, Jinyu
Jia, Haokang
Miao, Yutong
Hou, Maolin
Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences
title Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences
title_full Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences
title_fullStr Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences
title_full_unstemmed Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences
title_short Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences
title_sort sogatella furcifera saliva mucin-like protein is required for feeding and induces rice defences
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332473/
https://www.ncbi.nlm.nih.gov/pubmed/35897828
http://dx.doi.org/10.3390/ijms23158239
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