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Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography

Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous c...

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Autores principales: Guo, Sen, Cui, Xueqin, Jiang, Mi, Bai, Lu, Tian, Xiao, Guo, Tiantian, Liu, Qingchao, Zhang, Li, Ho, Chi-Tang, Bai, Naisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332516/
https://www.ncbi.nlm.nih.gov/pubmed/28911685
http://dx.doi.org/10.1016/j.jfda.2016.05.008
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author Guo, Sen
Cui, Xueqin
Jiang, Mi
Bai, Lu
Tian, Xiao
Guo, Tiantian
Liu, Qingchao
Zhang, Li
Ho, Chi-Tang
Bai, Naisheng
author_facet Guo, Sen
Cui, Xueqin
Jiang, Mi
Bai, Lu
Tian, Xiao
Guo, Tiantian
Liu, Qingchao
Zhang, Li
Ho, Chi-Tang
Bai, Naisheng
author_sort Guo, Sen
collection PubMed
description Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous characterization and quantification of 17 main constituents from four different cultivation regions in China. Under optimal conditions, analysis was performed on a Luna C-18 column and gradient elution with a solvent system of acetonitrile and 0.5% (v/v) acetic acid–water at a flow rate of 1.0 mL/min and wavelength of 220 nm and 280 nm. All standard calibration curves exhibited good linearity (r(2) > 0.9992) within the test ranges. The precision was evaluated by intraday and interday tests, which revealed relative standard deviation values within the ranges of 0.57–2.35% and 0.52–3.40%, respectively. The recoveries were in the range of 96.37–101.66%. The relative standard deviation values for stability and repeatability were < 5%. The contents of some compounds were low and varied with different cultivars. The proposed method could serve as a prerequisite for quality control of R. rubescens materials and products.
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spelling pubmed-93325162022-08-09 Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography Guo, Sen Cui, Xueqin Jiang, Mi Bai, Lu Tian, Xiao Guo, Tiantian Liu, Qingchao Zhang, Li Ho, Chi-Tang Bai, Naisheng J Food Drug Anal Original Article Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous characterization and quantification of 17 main constituents from four different cultivation regions in China. Under optimal conditions, analysis was performed on a Luna C-18 column and gradient elution with a solvent system of acetonitrile and 0.5% (v/v) acetic acid–water at a flow rate of 1.0 mL/min and wavelength of 220 nm and 280 nm. All standard calibration curves exhibited good linearity (r(2) > 0.9992) within the test ranges. The precision was evaluated by intraday and interday tests, which revealed relative standard deviation values within the ranges of 0.57–2.35% and 0.52–3.40%, respectively. The recoveries were in the range of 96.37–101.66%. The relative standard deviation values for stability and repeatability were < 5%. The contents of some compounds were low and varied with different cultivars. The proposed method could serve as a prerequisite for quality control of R. rubescens materials and products. Taiwan Food and Drug Administration 2016-07-26 /pmc/articles/PMC9332516/ /pubmed/28911685 http://dx.doi.org/10.1016/j.jfda.2016.05.008 Text en © 2017 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Guo, Sen
Cui, Xueqin
Jiang, Mi
Bai, Lu
Tian, Xiao
Guo, Tiantian
Liu, Qingchao
Zhang, Li
Ho, Chi-Tang
Bai, Naisheng
Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
title Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
title_full Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
title_fullStr Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
title_full_unstemmed Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
title_short Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
title_sort simultaneous characterization and quantification of 17 main compounds in rabdosia rubescens by high performance liquid chromatography
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332516/
https://www.ncbi.nlm.nih.gov/pubmed/28911685
http://dx.doi.org/10.1016/j.jfda.2016.05.008
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