Cargando…
Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography
Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous c...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taiwan Food and Drug Administration
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332516/ https://www.ncbi.nlm.nih.gov/pubmed/28911685 http://dx.doi.org/10.1016/j.jfda.2016.05.008 |
_version_ | 1784758666913644544 |
---|---|
author | Guo, Sen Cui, Xueqin Jiang, Mi Bai, Lu Tian, Xiao Guo, Tiantian Liu, Qingchao Zhang, Li Ho, Chi-Tang Bai, Naisheng |
author_facet | Guo, Sen Cui, Xueqin Jiang, Mi Bai, Lu Tian, Xiao Guo, Tiantian Liu, Qingchao Zhang, Li Ho, Chi-Tang Bai, Naisheng |
author_sort | Guo, Sen |
collection | PubMed |
description | Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous characterization and quantification of 17 main constituents from four different cultivation regions in China. Under optimal conditions, analysis was performed on a Luna C-18 column and gradient elution with a solvent system of acetonitrile and 0.5% (v/v) acetic acid–water at a flow rate of 1.0 mL/min and wavelength of 220 nm and 280 nm. All standard calibration curves exhibited good linearity (r(2) > 0.9992) within the test ranges. The precision was evaluated by intraday and interday tests, which revealed relative standard deviation values within the ranges of 0.57–2.35% and 0.52–3.40%, respectively. The recoveries were in the range of 96.37–101.66%. The relative standard deviation values for stability and repeatability were < 5%. The contents of some compounds were low and varied with different cultivars. The proposed method could serve as a prerequisite for quality control of R. rubescens materials and products. |
format | Online Article Text |
id | pubmed-9332516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taiwan Food and Drug Administration |
record_format | MEDLINE/PubMed |
spelling | pubmed-93325162022-08-09 Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography Guo, Sen Cui, Xueqin Jiang, Mi Bai, Lu Tian, Xiao Guo, Tiantian Liu, Qingchao Zhang, Li Ho, Chi-Tang Bai, Naisheng J Food Drug Anal Original Article Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous characterization and quantification of 17 main constituents from four different cultivation regions in China. Under optimal conditions, analysis was performed on a Luna C-18 column and gradient elution with a solvent system of acetonitrile and 0.5% (v/v) acetic acid–water at a flow rate of 1.0 mL/min and wavelength of 220 nm and 280 nm. All standard calibration curves exhibited good linearity (r(2) > 0.9992) within the test ranges. The precision was evaluated by intraday and interday tests, which revealed relative standard deviation values within the ranges of 0.57–2.35% and 0.52–3.40%, respectively. The recoveries were in the range of 96.37–101.66%. The relative standard deviation values for stability and repeatability were < 5%. The contents of some compounds were low and varied with different cultivars. The proposed method could serve as a prerequisite for quality control of R. rubescens materials and products. Taiwan Food and Drug Administration 2016-07-26 /pmc/articles/PMC9332516/ /pubmed/28911685 http://dx.doi.org/10.1016/j.jfda.2016.05.008 Text en © 2017 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Guo, Sen Cui, Xueqin Jiang, Mi Bai, Lu Tian, Xiao Guo, Tiantian Liu, Qingchao Zhang, Li Ho, Chi-Tang Bai, Naisheng Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography |
title | Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography |
title_full | Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography |
title_fullStr | Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography |
title_full_unstemmed | Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography |
title_short | Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography |
title_sort | simultaneous characterization and quantification of 17 main compounds in rabdosia rubescens by high performance liquid chromatography |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332516/ https://www.ncbi.nlm.nih.gov/pubmed/28911685 http://dx.doi.org/10.1016/j.jfda.2016.05.008 |
work_keys_str_mv | AT guosen simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT cuixueqin simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT jiangmi simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT bailu simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT tianxiao simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT guotiantian simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT liuqingchao simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT zhangli simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT hochitang simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography AT bainaisheng simultaneouscharacterizationandquantificationof17maincompoundsinrabdosiarubescensbyhighperformanceliquidchromatography |