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Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family

This study was undertaken to isolate and quantify aristolochic acid in Aristolochia indica stem and Apama siliquosa root. Aristolochic acid is an important biomarker component present in the Aristolochiaceae family. The isolation method involved simple solvent extraction, precipitation and further p...

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Detalles Bibliográficos
Autores principales: Agrawal, Poonam, Laddha, Kirti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332524/
https://www.ncbi.nlm.nih.gov/pubmed/28911686
http://dx.doi.org/10.1016/j.jfda.2016.06.006
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author Agrawal, Poonam
Laddha, Kirti
author_facet Agrawal, Poonam
Laddha, Kirti
author_sort Agrawal, Poonam
collection PubMed
description This study was undertaken to isolate and quantify aristolochic acid in Aristolochia indica stem and Apama siliquosa root. Aristolochic acid is an important biomarker component present in the Aristolochiaceae family. The isolation method involved simple solvent extraction, precipitation and further purification, using recrystallization. The structure of the compound was confirmed using infrared spectroscopy, mass spectrometry and nuclear magnetic resonance. A specific and rapid high-performance thin layer chromatography (HPTLC) method was developed for analysis of aristolochic acid. The method involved separation on the silica gel 60 F(254) plates using the single solvent system of n-hexane: chloroform: methanol. The method showed good linear relationship in the range 0.4–2.0 μg/spot with r(2) = 0.998. The limit of detection and limit of quantification were 62.841 ng/spot and 209.47 ng/spot, respectively. The proposed validated HPTLC method was found to be an easy to use, accurate and convenient method that could be successfully used for standardization and quality assessment of herbal material as well as formulations containing different species of the Aristolochiaceae family.
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spelling pubmed-93325242022-08-09 Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family Agrawal, Poonam Laddha, Kirti J Food Drug Anal Original Article This study was undertaken to isolate and quantify aristolochic acid in Aristolochia indica stem and Apama siliquosa root. Aristolochic acid is an important biomarker component present in the Aristolochiaceae family. The isolation method involved simple solvent extraction, precipitation and further purification, using recrystallization. The structure of the compound was confirmed using infrared spectroscopy, mass spectrometry and nuclear magnetic resonance. A specific and rapid high-performance thin layer chromatography (HPTLC) method was developed for analysis of aristolochic acid. The method involved separation on the silica gel 60 F(254) plates using the single solvent system of n-hexane: chloroform: methanol. The method showed good linear relationship in the range 0.4–2.0 μg/spot with r(2) = 0.998. The limit of detection and limit of quantification were 62.841 ng/spot and 209.47 ng/spot, respectively. The proposed validated HPTLC method was found to be an easy to use, accurate and convenient method that could be successfully used for standardization and quality assessment of herbal material as well as formulations containing different species of the Aristolochiaceae family. Taiwan Food and Drug Administration 2016-07-25 /pmc/articles/PMC9332524/ /pubmed/28911686 http://dx.doi.org/10.1016/j.jfda.2016.06.006 Text en © 2017 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Agrawal, Poonam
Laddha, Kirti
Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family
title Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family
title_full Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family
title_fullStr Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family
title_full_unstemmed Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family
title_short Development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the Aristolochiaceae family
title_sort development of validated high-performance thin layer chromatography for quantification of aristolochic acid in different species of the aristolochiaceae family
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332524/
https://www.ncbi.nlm.nih.gov/pubmed/28911686
http://dx.doi.org/10.1016/j.jfda.2016.06.006
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