Cargando…

Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer

Chlorogenic acid (CGA) has been reported to have various biological activities, such as anti-inflammatory, anti-oxidant and anti-apoptosis effects. However, the role of CGA in intracerebral hemorrhage (ICH) and the underlying mechanisms remain undiscovered. The current study aims to investigate the...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yang, Wang, Fei, Li, Zhe, Mu, Yanling, Yong, Voon Wee, Xue, Mengzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332591/
https://www.ncbi.nlm.nih.gov/pubmed/35892330
http://dx.doi.org/10.3390/biom12081020
_version_ 1784758685667426304
author Liu, Yang
Wang, Fei
Li, Zhe
Mu, Yanling
Yong, Voon Wee
Xue, Mengzhou
author_facet Liu, Yang
Wang, Fei
Li, Zhe
Mu, Yanling
Yong, Voon Wee
Xue, Mengzhou
author_sort Liu, Yang
collection PubMed
description Chlorogenic acid (CGA) has been reported to have various biological activities, such as anti-inflammatory, anti-oxidant and anti-apoptosis effects. However, the role of CGA in intracerebral hemorrhage (ICH) and the underlying mechanisms remain undiscovered. The current study aims to investigate the effect of CGA on neuroinflammation and neuronal apoptosis after inhibition of EMMPRIN in a collagenase-induced ICH mouse model. Dose optimization data showed that intraperitoneal administration of CGA (30 mg/kg) significantly attenuated neurological impairments and reduced brain water content at 24 h and 72 h compared with ICH mice given vehicle. Western blot and immunofluorescence analyses revealed that CGA remarkably decreased the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in perihematomal areas at 72 h after ICH. CGA also reduced the expression of matrix metalloproteinases-2/9 (MMP-2/9) at 72 h after ICH. CGA diminished Evans blue dye extravasation and reduced the loss of zonula occludens-1 (ZO-1) and occludin. CGA-treated mice had fewer activated Iba-1-positive microglia and MPO-positive neutrophils. Finally, CGA suppressed cell death around the hematoma and reduced overall brain injury. These outcomes highlight that CGA treatment confers neuroprotection in ICH likely by inhibiting expression of EMMPRIN and MMP-2/9, and alleviating neuroinflammation, blood–brain barrier (BBB) disruption, cell death and brain injury.
format Online
Article
Text
id pubmed-9332591
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93325912022-07-29 Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer Liu, Yang Wang, Fei Li, Zhe Mu, Yanling Yong, Voon Wee Xue, Mengzhou Biomolecules Article Chlorogenic acid (CGA) has been reported to have various biological activities, such as anti-inflammatory, anti-oxidant and anti-apoptosis effects. However, the role of CGA in intracerebral hemorrhage (ICH) and the underlying mechanisms remain undiscovered. The current study aims to investigate the effect of CGA on neuroinflammation and neuronal apoptosis after inhibition of EMMPRIN in a collagenase-induced ICH mouse model. Dose optimization data showed that intraperitoneal administration of CGA (30 mg/kg) significantly attenuated neurological impairments and reduced brain water content at 24 h and 72 h compared with ICH mice given vehicle. Western blot and immunofluorescence analyses revealed that CGA remarkably decreased the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in perihematomal areas at 72 h after ICH. CGA also reduced the expression of matrix metalloproteinases-2/9 (MMP-2/9) at 72 h after ICH. CGA diminished Evans blue dye extravasation and reduced the loss of zonula occludens-1 (ZO-1) and occludin. CGA-treated mice had fewer activated Iba-1-positive microglia and MPO-positive neutrophils. Finally, CGA suppressed cell death around the hematoma and reduced overall brain injury. These outcomes highlight that CGA treatment confers neuroprotection in ICH likely by inhibiting expression of EMMPRIN and MMP-2/9, and alleviating neuroinflammation, blood–brain barrier (BBB) disruption, cell death and brain injury. MDPI 2022-07-22 /pmc/articles/PMC9332591/ /pubmed/35892330 http://dx.doi.org/10.3390/biom12081020 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yang
Wang, Fei
Li, Zhe
Mu, Yanling
Yong, Voon Wee
Xue, Mengzhou
Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer
title Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer
title_full Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer
title_fullStr Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer
title_full_unstemmed Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer
title_short Neuroprotective Effects of Chlorogenic Acid in a Mouse Model of Intracerebral Hemorrhage Associated with Reduced Extracellular Matrix Metalloproteinase Inducer
title_sort neuroprotective effects of chlorogenic acid in a mouse model of intracerebral hemorrhage associated with reduced extracellular matrix metalloproteinase inducer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332591/
https://www.ncbi.nlm.nih.gov/pubmed/35892330
http://dx.doi.org/10.3390/biom12081020
work_keys_str_mv AT liuyang neuroprotectiveeffectsofchlorogenicacidinamousemodelofintracerebralhemorrhageassociatedwithreducedextracellularmatrixmetalloproteinaseinducer
AT wangfei neuroprotectiveeffectsofchlorogenicacidinamousemodelofintracerebralhemorrhageassociatedwithreducedextracellularmatrixmetalloproteinaseinducer
AT lizhe neuroprotectiveeffectsofchlorogenicacidinamousemodelofintracerebralhemorrhageassociatedwithreducedextracellularmatrixmetalloproteinaseinducer
AT muyanling neuroprotectiveeffectsofchlorogenicacidinamousemodelofintracerebralhemorrhageassociatedwithreducedextracellularmatrixmetalloproteinaseinducer
AT yongvoonwee neuroprotectiveeffectsofchlorogenicacidinamousemodelofintracerebralhemorrhageassociatedwithreducedextracellularmatrixmetalloproteinaseinducer
AT xuemengzhou neuroprotectiveeffectsofchlorogenicacidinamousemodelofintracerebralhemorrhageassociatedwithreducedextracellularmatrixmetalloproteinaseinducer