Cargando…
Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant
Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and pr...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332613/ https://www.ncbi.nlm.nih.gov/pubmed/35893641 http://dx.doi.org/10.3390/plants11151937 |
_version_ | 1784758690872557568 |
---|---|
author | Gu, Min Li, Youli Jiang, Huier Zhang, Shihu Que, Qingmin Chen, Xiaoyang Zhou, Wei |
author_facet | Gu, Min Li, Youli Jiang, Huier Zhang, Shihu Que, Qingmin Chen, Xiaoyang Zhou, Wei |
author_sort | Gu, Min |
collection | PubMed |
description | Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (p < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. |
format | Online Article Text |
id | pubmed-9332613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93326132022-07-29 Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant Gu, Min Li, Youli Jiang, Huier Zhang, Shihu Que, Qingmin Chen, Xiaoyang Zhou, Wei Plants (Basel) Article Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (p < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. MDPI 2022-07-26 /pmc/articles/PMC9332613/ /pubmed/35893641 http://dx.doi.org/10.3390/plants11151937 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gu, Min Li, Youli Jiang, Huier Zhang, Shihu Que, Qingmin Chen, Xiaoyang Zhou, Wei Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant |
title | Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant |
title_full | Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant |
title_fullStr | Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant |
title_full_unstemmed | Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant |
title_short | Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant |
title_sort | efficient in vitro sterilization and propagation from stem segment explants of cnidoscolus aconitifolius (mill.) i.m. johnst, a multipurpose woody plant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332613/ https://www.ncbi.nlm.nih.gov/pubmed/35893641 http://dx.doi.org/10.3390/plants11151937 |
work_keys_str_mv | AT gumin efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant AT liyouli efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant AT jianghuier efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant AT zhangshihu efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant AT queqingmin efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant AT chenxiaoyang efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant AT zhouwei efficientinvitrosterilizationandpropagationfromstemsegmentexplantsofcnidoscolusaconitifoliusmillimjohnstamultipurposewoodyplant |