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Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production
Polygonum cuspidatum is a widely grown crop with a rich source of polydatin (also called piceid) for resveratrol production. Resveratrol is produced from piceid via enzymatic cleavage of the sugar moiety of piceid. In this study, Dekkera bruxellensis mutants were selected based on their high p-nitro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Taiwan Food and Drug Administration
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332651/ https://www.ncbi.nlm.nih.gov/pubmed/29389552 http://dx.doi.org/10.1016/j.jfda.2016.12.016 |
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author | Kuo, Hsiao-Ping Wang, Reuben Huang, Chiao-Ying Lai, Jinn-Tsyy Lo, Yi-Chen Huang, Shyue-Tsong |
author_facet | Kuo, Hsiao-Ping Wang, Reuben Huang, Chiao-Ying Lai, Jinn-Tsyy Lo, Yi-Chen Huang, Shyue-Tsong |
author_sort | Kuo, Hsiao-Ping |
collection | PubMed |
description | Polygonum cuspidatum is a widely grown crop with a rich source of polydatin (also called piceid) for resveratrol production. Resveratrol is produced from piceid via enzymatic cleavage of the sugar moiety of piceid. In this study, Dekkera bruxellensis mutants were selected based on their high p-nitrophenyl-β-d-glucopyranoside and piceid conversion activities. The enzyme responsible for piceid conversion was a heterodimeric protein complex that was predominantly secreted to the extracellular medium and consisted of two subunits at an equal ratio with molecular masses of 30.5 kDa and 48.3 kDa. The two subunits were identified as SCW4p and glucan-β-glucosidase precursor in D. bruxellensis. Both proteins were individually expressed in Saccharomyces cerevisiae exg1Δ mutants, which lack extracellular β-glucosidase activity, to confirm each protein’s enzymatic activities. Only the glucan-β-glucosidase precursor was shown to be a secretory protein with piceid deglycosylation activity. Our pilot experiments of piceid bioconversion demonstrate the possible industrial applications for this glucan-β-glucosidase precursor in the future. |
format | Online Article Text |
id | pubmed-9332651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taiwan Food and Drug Administration |
record_format | MEDLINE/PubMed |
spelling | pubmed-93326512022-08-09 Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production Kuo, Hsiao-Ping Wang, Reuben Huang, Chiao-Ying Lai, Jinn-Tsyy Lo, Yi-Chen Huang, Shyue-Tsong J Food Drug Anal Original Article Polygonum cuspidatum is a widely grown crop with a rich source of polydatin (also called piceid) for resveratrol production. Resveratrol is produced from piceid via enzymatic cleavage of the sugar moiety of piceid. In this study, Dekkera bruxellensis mutants were selected based on their high p-nitrophenyl-β-d-glucopyranoside and piceid conversion activities. The enzyme responsible for piceid conversion was a heterodimeric protein complex that was predominantly secreted to the extracellular medium and consisted of two subunits at an equal ratio with molecular masses of 30.5 kDa and 48.3 kDa. The two subunits were identified as SCW4p and glucan-β-glucosidase precursor in D. bruxellensis. Both proteins were individually expressed in Saccharomyces cerevisiae exg1Δ mutants, which lack extracellular β-glucosidase activity, to confirm each protein’s enzymatic activities. Only the glucan-β-glucosidase precursor was shown to be a secretory protein with piceid deglycosylation activity. Our pilot experiments of piceid bioconversion demonstrate the possible industrial applications for this glucan-β-glucosidase precursor in the future. Taiwan Food and Drug Administration 2017-02-21 /pmc/articles/PMC9332651/ /pubmed/29389552 http://dx.doi.org/10.1016/j.jfda.2016.12.016 Text en © 2018 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Kuo, Hsiao-Ping Wang, Reuben Huang, Chiao-Ying Lai, Jinn-Tsyy Lo, Yi-Chen Huang, Shyue-Tsong Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production |
title | Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production |
title_full | Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production |
title_fullStr | Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production |
title_full_unstemmed | Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production |
title_short | Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production |
title_sort | characterization of an extracellular β-glucosidase from dekkera bruxellensis for resveratrol production |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332651/ https://www.ncbi.nlm.nih.gov/pubmed/29389552 http://dx.doi.org/10.1016/j.jfda.2016.12.016 |
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