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Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation

This study presents a set of vibrational characterizations on a nanogel-cross-linked porous freeze-dried gel (NanoCliP-FD gel) scaffold for tissue engineering and regenerative therapy. This scaffold is designed for the in vitro culture of high-quality cartilage tissue to be then transplanted in vivo...

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Autores principales: Adachi, Tetsuya, Miyamoto, Nao, Imamura, Hayata, Yamamoto, Toshiro, Marin, Elia, Zhu, Wenliang, Kobara, Miyuki, Sowa, Yoshihiro, Tahara, Yoshiro, Kanamura, Narisato, Akiyoshi, Kazunari, Mazda, Osam, Nishimura, Ichiro, Pezzotti, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332688/
https://www.ncbi.nlm.nih.gov/pubmed/35897669
http://dx.doi.org/10.3390/ijms23158099
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author Adachi, Tetsuya
Miyamoto, Nao
Imamura, Hayata
Yamamoto, Toshiro
Marin, Elia
Zhu, Wenliang
Kobara, Miyuki
Sowa, Yoshihiro
Tahara, Yoshiro
Kanamura, Narisato
Akiyoshi, Kazunari
Mazda, Osam
Nishimura, Ichiro
Pezzotti, Giuseppe
author_facet Adachi, Tetsuya
Miyamoto, Nao
Imamura, Hayata
Yamamoto, Toshiro
Marin, Elia
Zhu, Wenliang
Kobara, Miyuki
Sowa, Yoshihiro
Tahara, Yoshiro
Kanamura, Narisato
Akiyoshi, Kazunari
Mazda, Osam
Nishimura, Ichiro
Pezzotti, Giuseppe
author_sort Adachi, Tetsuya
collection PubMed
description This study presents a set of vibrational characterizations on a nanogel-cross-linked porous freeze-dried gel (NanoCliP-FD gel) scaffold for tissue engineering and regenerative therapy. This scaffold is designed for the in vitro culture of high-quality cartilage tissue to be then transplanted in vivo to enable recovery from congenital malformations in the maxillofacial area or crippling jaw disease. The three-dimensional scaffold for in-plate culture is designed with interface chemistry capable of stimulating cartilage formation and maintaining its structure through counteracting the dedifferentiation of mesenchymal stem cells (MSCs) during the formation of cartilage tissue. The developed interface chemistry enabled high efficiency in both growth rate and tissue quality, thus satisfying the requirements of large volumes, high matrix quality, and superior mechanical properties needed in cartilage transplants. We characterized the cartilage tissue in vitro grown on a NanoCliP-FD gel scaffold by human periodontal ligament-derived stem cells (a type of MSC) with cartilage grown by the same cells and under the same conditions on a conventional (porous) atelocollagen scaffold. The cartilage tissues produced by the MSCs on different scaffolds were comparatively evaluated by immunohistochemical and spectroscopic analyses. Cartilage differentiation occurred at a higher rate when MSCs were cultured on the NanoCliP-FD gel scaffold compared to the atelocollagen scaffold, and produced a tissue richer in cartilage matrix. In situ spectroscopic analyses revealed the cell/scaffold interactive mechanisms by which the NanoCliP-FD gel scaffold stimulated such increased efficiency in cartilage matrix formation. In addition to demonstrating the high potential of human periodontal ligament-derived stem cell cultures on NanoCliP-FD gel scaffolds in regenerative cartilage therapy, the present study also highlights the novelty of Raman spectroscopy as a non-destructive method for the concurrent evaluation of matrix quality and cell metabolic response. In situ Raman analyses on living cells unveiled for the first time the underlying physiological mechanisms behind such improved chondrocyte performance.
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spelling pubmed-93326882022-07-29 Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation Adachi, Tetsuya Miyamoto, Nao Imamura, Hayata Yamamoto, Toshiro Marin, Elia Zhu, Wenliang Kobara, Miyuki Sowa, Yoshihiro Tahara, Yoshiro Kanamura, Narisato Akiyoshi, Kazunari Mazda, Osam Nishimura, Ichiro Pezzotti, Giuseppe Int J Mol Sci Article This study presents a set of vibrational characterizations on a nanogel-cross-linked porous freeze-dried gel (NanoCliP-FD gel) scaffold for tissue engineering and regenerative therapy. This scaffold is designed for the in vitro culture of high-quality cartilage tissue to be then transplanted in vivo to enable recovery from congenital malformations in the maxillofacial area or crippling jaw disease. The three-dimensional scaffold for in-plate culture is designed with interface chemistry capable of stimulating cartilage formation and maintaining its structure through counteracting the dedifferentiation of mesenchymal stem cells (MSCs) during the formation of cartilage tissue. The developed interface chemistry enabled high efficiency in both growth rate and tissue quality, thus satisfying the requirements of large volumes, high matrix quality, and superior mechanical properties needed in cartilage transplants. We characterized the cartilage tissue in vitro grown on a NanoCliP-FD gel scaffold by human periodontal ligament-derived stem cells (a type of MSC) with cartilage grown by the same cells and under the same conditions on a conventional (porous) atelocollagen scaffold. The cartilage tissues produced by the MSCs on different scaffolds were comparatively evaluated by immunohistochemical and spectroscopic analyses. Cartilage differentiation occurred at a higher rate when MSCs were cultured on the NanoCliP-FD gel scaffold compared to the atelocollagen scaffold, and produced a tissue richer in cartilage matrix. In situ spectroscopic analyses revealed the cell/scaffold interactive mechanisms by which the NanoCliP-FD gel scaffold stimulated such increased efficiency in cartilage matrix formation. In addition to demonstrating the high potential of human periodontal ligament-derived stem cell cultures on NanoCliP-FD gel scaffolds in regenerative cartilage therapy, the present study also highlights the novelty of Raman spectroscopy as a non-destructive method for the concurrent evaluation of matrix quality and cell metabolic response. In situ Raman analyses on living cells unveiled for the first time the underlying physiological mechanisms behind such improved chondrocyte performance. MDPI 2022-07-22 /pmc/articles/PMC9332688/ /pubmed/35897669 http://dx.doi.org/10.3390/ijms23158099 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adachi, Tetsuya
Miyamoto, Nao
Imamura, Hayata
Yamamoto, Toshiro
Marin, Elia
Zhu, Wenliang
Kobara, Miyuki
Sowa, Yoshihiro
Tahara, Yoshiro
Kanamura, Narisato
Akiyoshi, Kazunari
Mazda, Osam
Nishimura, Ichiro
Pezzotti, Giuseppe
Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation
title Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation
title_full Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation
title_fullStr Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation
title_full_unstemmed Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation
title_short Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation
title_sort three-dimensional culture of cartilage tissue on nanogel-cross-linked porous freeze-dried gel scaffold for regenerative cartilage therapy: a vibrational spectroscopy evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332688/
https://www.ncbi.nlm.nih.gov/pubmed/35897669
http://dx.doi.org/10.3390/ijms23158099
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