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Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model

Exposure to particulate matter (PM) has been linked with the severity of various diseases. To date, there is no study on the relationship between PM exposure and tendon healing. Open Achilles tenotomy of 20 rats was performed. The animals were divided into two groups according to exposure to PM: a P...

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Autores principales: Lee, Su-Yel, Lee, Min-Hyeok, Jo, Seong-Kyeong, Yoo, In-Ha, Sarankhuu, Boler-Erdene, Kim, Hyun-Jin, Kang, Yea-Eun, Lee, Seong-Eun, Kim, Tae-Yeon, Park, Moon-Hyang, Lee, Choong-Sik, Han, Seung-Yun, Moon, Ji-Hyun, Jung, Ju-Young, Hong, Geum-Lan, Yoo, Nam-Jeong, Yoon, Eun-Sang, Choi, Jae-Kyu, Won, Ho-Ryun, Son, Ji-Woong, Song, Jae-Hwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332732/
https://www.ncbi.nlm.nih.gov/pubmed/35897746
http://dx.doi.org/10.3390/ijms23158170
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author Lee, Su-Yel
Lee, Min-Hyeok
Jo, Seong-Kyeong
Yoo, In-Ha
Sarankhuu, Boler-Erdene
Kim, Hyun-Jin
Kang, Yea-Eun
Lee, Seong-Eun
Kim, Tae-Yeon
Park, Moon-Hyang
Lee, Choong-Sik
Han, Seung-Yun
Moon, Ji-Hyun
Jung, Ju-Young
Hong, Geum-Lan
Yoo, Nam-Jeong
Yoon, Eun-Sang
Choi, Jae-Kyu
Won, Ho-Ryun
Son, Ji-Woong
Song, Jae-Hwang
author_facet Lee, Su-Yel
Lee, Min-Hyeok
Jo, Seong-Kyeong
Yoo, In-Ha
Sarankhuu, Boler-Erdene
Kim, Hyun-Jin
Kang, Yea-Eun
Lee, Seong-Eun
Kim, Tae-Yeon
Park, Moon-Hyang
Lee, Choong-Sik
Han, Seung-Yun
Moon, Ji-Hyun
Jung, Ju-Young
Hong, Geum-Lan
Yoo, Nam-Jeong
Yoon, Eun-Sang
Choi, Jae-Kyu
Won, Ho-Ryun
Son, Ji-Woong
Song, Jae-Hwang
author_sort Lee, Su-Yel
collection PubMed
description Exposure to particulate matter (PM) has been linked with the severity of various diseases. To date, there is no study on the relationship between PM exposure and tendon healing. Open Achilles tenotomy of 20 rats was performed. The animals were divided into two groups according to exposure to PM: a PM group and a non-PM group. After 6 weeks of PM exposure, the harvest and investigations of lungs, blood samples, and Achilles tendons were performed. Compared to the non-PM group, the white blood cell count and tumor necrosis factor-alpha expression in the PM group were significantly higher. The Achilles tendons in PM group showed significantly increased inflammatory outcomes. A TEM analysis showed reduced collagen fibrils in the PM group. A biomechanical analysis demonstrated that the load to failure value was lower in the PM group. An upregulation of the gene encoding cyclic AMP response element-binding protein (CREB) was detected in the PM group by an integrated analysis of DNA methylation and RNA sequencing data, as confirmed via a Western blot analysis showing significantly elevated levels of phosphorylated CREB. In summary, PM exposure caused a deleterious effect on tendon healing. The molecular data indicate that the action mechanism of PM may be associated with upregulated CREB signaling.
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spelling pubmed-93327322022-07-29 Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model Lee, Su-Yel Lee, Min-Hyeok Jo, Seong-Kyeong Yoo, In-Ha Sarankhuu, Boler-Erdene Kim, Hyun-Jin Kang, Yea-Eun Lee, Seong-Eun Kim, Tae-Yeon Park, Moon-Hyang Lee, Choong-Sik Han, Seung-Yun Moon, Ji-Hyun Jung, Ju-Young Hong, Geum-Lan Yoo, Nam-Jeong Yoon, Eun-Sang Choi, Jae-Kyu Won, Ho-Ryun Son, Ji-Woong Song, Jae-Hwang Int J Mol Sci Article Exposure to particulate matter (PM) has been linked with the severity of various diseases. To date, there is no study on the relationship between PM exposure and tendon healing. Open Achilles tenotomy of 20 rats was performed. The animals were divided into two groups according to exposure to PM: a PM group and a non-PM group. After 6 weeks of PM exposure, the harvest and investigations of lungs, blood samples, and Achilles tendons were performed. Compared to the non-PM group, the white blood cell count and tumor necrosis factor-alpha expression in the PM group were significantly higher. The Achilles tendons in PM group showed significantly increased inflammatory outcomes. A TEM analysis showed reduced collagen fibrils in the PM group. A biomechanical analysis demonstrated that the load to failure value was lower in the PM group. An upregulation of the gene encoding cyclic AMP response element-binding protein (CREB) was detected in the PM group by an integrated analysis of DNA methylation and RNA sequencing data, as confirmed via a Western blot analysis showing significantly elevated levels of phosphorylated CREB. In summary, PM exposure caused a deleterious effect on tendon healing. The molecular data indicate that the action mechanism of PM may be associated with upregulated CREB signaling. MDPI 2022-07-25 /pmc/articles/PMC9332732/ /pubmed/35897746 http://dx.doi.org/10.3390/ijms23158170 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Su-Yel
Lee, Min-Hyeok
Jo, Seong-Kyeong
Yoo, In-Ha
Sarankhuu, Boler-Erdene
Kim, Hyun-Jin
Kang, Yea-Eun
Lee, Seong-Eun
Kim, Tae-Yeon
Park, Moon-Hyang
Lee, Choong-Sik
Han, Seung-Yun
Moon, Ji-Hyun
Jung, Ju-Young
Hong, Geum-Lan
Yoo, Nam-Jeong
Yoon, Eun-Sang
Choi, Jae-Kyu
Won, Ho-Ryun
Son, Ji-Woong
Song, Jae-Hwang
Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model
title Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model
title_full Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model
title_fullStr Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model
title_full_unstemmed Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model
title_short Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model
title_sort effects and mechanism of particulate matter on tendon healing based on integrated analysis of dna methylation and rna sequencing data in a rat model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332732/
https://www.ncbi.nlm.nih.gov/pubmed/35897746
http://dx.doi.org/10.3390/ijms23158170
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