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The dynamic network of IS30 transposition pathways

The E. coli element IS30 has adopted the copy-out-paste-in transposition mechanism that is prevalent in a number of IS-families. As an initial step, IS30 forms free circular transposition intermediates like IS minicircles or tandem IS-dimers by joining the inverted repeats of a single element or two...

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Autores principales: Olasz, Ferenc, Szabó, Mónika, Veress, Alexandra, Bibó, Márton, Kiss, János
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333248/
https://www.ncbi.nlm.nih.gov/pubmed/35901099
http://dx.doi.org/10.1371/journal.pone.0271414
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author Olasz, Ferenc
Szabó, Mónika
Veress, Alexandra
Bibó, Márton
Kiss, János
author_facet Olasz, Ferenc
Szabó, Mónika
Veress, Alexandra
Bibó, Márton
Kiss, János
author_sort Olasz, Ferenc
collection PubMed
description The E. coli element IS30 has adopted the copy-out-paste-in transposition mechanism that is prevalent in a number of IS-families. As an initial step, IS30 forms free circular transposition intermediates like IS minicircles or tandem IS-dimers by joining the inverted repeats of a single element or two, sometimes distantly positioned IS copies, respectively. Then, the active IR-IR junction of these intermediates reacts with the target DNA, which generates insertions, deletions, inversions or cointegrates. The element shows dual target specificity as it can insert into hot spot sequences or next to its inverted repeats. In this study the pathways of rearrangements of transposition-derived cointegrate-like structures were examined. The results showed that the probability of further rearrangements in these structures depends on whether the IS elements are flanked by hot spot sequences or take part in an IR-IR junction. The variability of the deriving products increases with the number of simultaneously available IRs and IR-IR joints in the cointegrates or the chromosome. Under certain conditions, the parental structures whose transposition formed the cointegrates are restored and persist among the rearranged products. Based on these findings, a novel dynamic model has been proposed for IS30, which possibly fits to other elements that have adopted the same transposition mechanism. The model integrates the known transposition pathways and the downstream rearrangements occurring after the formation of different cointegrate-like structures into a complex network. Important feature of this network is the presence of “feedback loops” and reversible transposition rearrangements that can explain how IS30 generates variability and preserves the original genetic constitution in the bacterial population, which contributes to the adaptability and evolution of host bacteria.
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spelling pubmed-93332482022-07-29 The dynamic network of IS30 transposition pathways Olasz, Ferenc Szabó, Mónika Veress, Alexandra Bibó, Márton Kiss, János PLoS One Research Article The E. coli element IS30 has adopted the copy-out-paste-in transposition mechanism that is prevalent in a number of IS-families. As an initial step, IS30 forms free circular transposition intermediates like IS minicircles or tandem IS-dimers by joining the inverted repeats of a single element or two, sometimes distantly positioned IS copies, respectively. Then, the active IR-IR junction of these intermediates reacts with the target DNA, which generates insertions, deletions, inversions or cointegrates. The element shows dual target specificity as it can insert into hot spot sequences or next to its inverted repeats. In this study the pathways of rearrangements of transposition-derived cointegrate-like structures were examined. The results showed that the probability of further rearrangements in these structures depends on whether the IS elements are flanked by hot spot sequences or take part in an IR-IR junction. The variability of the deriving products increases with the number of simultaneously available IRs and IR-IR joints in the cointegrates or the chromosome. Under certain conditions, the parental structures whose transposition formed the cointegrates are restored and persist among the rearranged products. Based on these findings, a novel dynamic model has been proposed for IS30, which possibly fits to other elements that have adopted the same transposition mechanism. The model integrates the known transposition pathways and the downstream rearrangements occurring after the formation of different cointegrate-like structures into a complex network. Important feature of this network is the presence of “feedback loops” and reversible transposition rearrangements that can explain how IS30 generates variability and preserves the original genetic constitution in the bacterial population, which contributes to the adaptability and evolution of host bacteria. Public Library of Science 2022-07-28 /pmc/articles/PMC9333248/ /pubmed/35901099 http://dx.doi.org/10.1371/journal.pone.0271414 Text en © 2022 Olasz et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Olasz, Ferenc
Szabó, Mónika
Veress, Alexandra
Bibó, Márton
Kiss, János
The dynamic network of IS30 transposition pathways
title The dynamic network of IS30 transposition pathways
title_full The dynamic network of IS30 transposition pathways
title_fullStr The dynamic network of IS30 transposition pathways
title_full_unstemmed The dynamic network of IS30 transposition pathways
title_short The dynamic network of IS30 transposition pathways
title_sort dynamic network of is30 transposition pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333248/
https://www.ncbi.nlm.nih.gov/pubmed/35901099
http://dx.doi.org/10.1371/journal.pone.0271414
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