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Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib

This project attempts to clarify the expression of MUS81 in castration-resistant prostate cancer (CRPC) and the effect on drug sensitivity to Olaparib. We collected clinical surgical samples of patients who were suffering from benign prostatic hyperplasia (BPH), common prostate cancer (PCa), and cas...

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Detalles Bibliográficos
Autores principales: Gong, Lifeng, Tang, Yu, Jiang, Li, Tang, Wei, Luo, Shengjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334051/
https://www.ncbi.nlm.nih.gov/pubmed/35910852
http://dx.doi.org/10.1155/2022/4065580
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author Gong, Lifeng
Tang, Yu
Jiang, Li
Tang, Wei
Luo, Shengjun
author_facet Gong, Lifeng
Tang, Yu
Jiang, Li
Tang, Wei
Luo, Shengjun
author_sort Gong, Lifeng
collection PubMed
description This project attempts to clarify the expression of MUS81 in castration-resistant prostate cancer (CRPC) and the effect on drug sensitivity to Olaparib. We collected clinical surgical samples of patients who were suffering from benign prostatic hyperplasia (BPH), common prostate cancer (PCa), and castration-resistant prostate cancer (CRPC) and detected the expression of MUS81 in healthy prostate epithelial cells, PCa cells, and androgen-independent PCa cells. We subsequently performed CCK-8 assays, flow cytometry, and Transwell invasion and migration assay to determine the proliferation, apoptosis, invasion, and metastasis abilities of transfected CRPC cells as well as drug toxicity of Olaparib to CRPC cells. The expression of MUS81 indicated marked upregulation in PCa and CRPC tissues, compared with the level of MUS81 in BPH tissues. MUS81 silencing inhibited the proliferation of CRPC cells and promoted their sensitivity to Olaparib. MUS81 silencing in CRPC cells remarkably accelerated cell apoptosis and greatly inhibited cell invasion and metastasis after Olaparib administration. MUS81 silencing in CRPC cells has significantly enhanced the sensitivity of cells to Olaparib, which provides evidence for the prediction of Olaparib resistance in CRPC cells by the MUS81 gene and is expected to become a promising gene target in CRPC therapy.
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spelling pubmed-93340512022-07-29 Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib Gong, Lifeng Tang, Yu Jiang, Li Tang, Wei Luo, Shengjun J Immunol Res Research Article This project attempts to clarify the expression of MUS81 in castration-resistant prostate cancer (CRPC) and the effect on drug sensitivity to Olaparib. We collected clinical surgical samples of patients who were suffering from benign prostatic hyperplasia (BPH), common prostate cancer (PCa), and castration-resistant prostate cancer (CRPC) and detected the expression of MUS81 in healthy prostate epithelial cells, PCa cells, and androgen-independent PCa cells. We subsequently performed CCK-8 assays, flow cytometry, and Transwell invasion and migration assay to determine the proliferation, apoptosis, invasion, and metastasis abilities of transfected CRPC cells as well as drug toxicity of Olaparib to CRPC cells. The expression of MUS81 indicated marked upregulation in PCa and CRPC tissues, compared with the level of MUS81 in BPH tissues. MUS81 silencing inhibited the proliferation of CRPC cells and promoted their sensitivity to Olaparib. MUS81 silencing in CRPC cells remarkably accelerated cell apoptosis and greatly inhibited cell invasion and metastasis after Olaparib administration. MUS81 silencing in CRPC cells has significantly enhanced the sensitivity of cells to Olaparib, which provides evidence for the prediction of Olaparib resistance in CRPC cells by the MUS81 gene and is expected to become a promising gene target in CRPC therapy. Hindawi 2022-07-21 /pmc/articles/PMC9334051/ /pubmed/35910852 http://dx.doi.org/10.1155/2022/4065580 Text en Copyright © 2022 Lifeng Gong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gong, Lifeng
Tang, Yu
Jiang, Li
Tang, Wei
Luo, Shengjun
Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib
title Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib
title_full Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib
title_fullStr Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib
title_full_unstemmed Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib
title_short Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib
title_sort expression of mus81 mediates the sensitivity of castration-resistant prostate cancer to olaparib
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334051/
https://www.ncbi.nlm.nih.gov/pubmed/35910852
http://dx.doi.org/10.1155/2022/4065580
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