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Sprouty 1 is associated with stemness and cancer progression in glioblastoma

Glioblastoma multiforme (GBM) is the most severe type of human brain tumor, with a poor prognosis and a low survival rate. GBM is composed of a variety of cell types, including glioma stem-like cells (GSCs), which attribute to its therapeutic resistance (Boyd et al., 2020). Sprouty1 (SPRY1) was firs...

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Detalles Bibliográficos
Autores principales: Park, Seo-Young, Jeong, Hang Yeon, Batara, Don Carlo, Lee, Suk Jun, Cho, Jeong-Yong, Kim, Sung-Hak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334334/
https://www.ncbi.nlm.nih.gov/pubmed/35910677
http://dx.doi.org/10.1016/j.ibneur.2022.07.003
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author Park, Seo-Young
Jeong, Hang Yeon
Batara, Don Carlo
Lee, Suk Jun
Cho, Jeong-Yong
Kim, Sung-Hak
author_facet Park, Seo-Young
Jeong, Hang Yeon
Batara, Don Carlo
Lee, Suk Jun
Cho, Jeong-Yong
Kim, Sung-Hak
author_sort Park, Seo-Young
collection PubMed
description Glioblastoma multiforme (GBM) is the most severe type of human brain tumor, with a poor prognosis and a low survival rate. GBM is composed of a variety of cell types, including glioma stem-like cells (GSCs), which attribute to its therapeutic resistance (Boyd et al., 2020). Sprouty1 (SPRY1) was first identified as a receptor tyrosine kinases (RTK) signaling mediator in a mammalian cell (Christofori, 2003), however, its role in GBM is unknown. Therefore, the goal of this study was to investigate the role of SPRY1 in the stemness and aggressiveness of GSCs. The mRNA expression levels of SPRY1 were confirmed using quantitative reverse transcription PCR (RT-qPCR) in normal human astrocytes (NHA), glioma cells, and glioma stem cells. SPRY1 expression was inhibited in glioma stem cells using small interference RNA (siRNAs) to examine its role in cell proliferation and tumorsphere formation. Bioinformatics analyses were also employed to investigate the association of SPRY1 expression with patient survival, tumor grade, and subtypes publicly available datasets. We demonstrated that SPRY1 is highly expressed in glioma stem cells than in NHA, glioma cells, and differentiated glioma stem cells. siRNA-mediated downregulation of SPRY1 expression decreased the stemness and self-renewal ability in GSC11. Bioinformatics results showed that high SPRY1 expression correlates with poor overall survival in glioma patients. Our findings suggest that SPRY1 contributes to the stemness and aggressiveness of GBM.
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spelling pubmed-93343342022-07-30 Sprouty 1 is associated with stemness and cancer progression in glioblastoma Park, Seo-Young Jeong, Hang Yeon Batara, Don Carlo Lee, Suk Jun Cho, Jeong-Yong Kim, Sung-Hak IBRO Neurosci Rep Research Paper Glioblastoma multiforme (GBM) is the most severe type of human brain tumor, with a poor prognosis and a low survival rate. GBM is composed of a variety of cell types, including glioma stem-like cells (GSCs), which attribute to its therapeutic resistance (Boyd et al., 2020). Sprouty1 (SPRY1) was first identified as a receptor tyrosine kinases (RTK) signaling mediator in a mammalian cell (Christofori, 2003), however, its role in GBM is unknown. Therefore, the goal of this study was to investigate the role of SPRY1 in the stemness and aggressiveness of GSCs. The mRNA expression levels of SPRY1 were confirmed using quantitative reverse transcription PCR (RT-qPCR) in normal human astrocytes (NHA), glioma cells, and glioma stem cells. SPRY1 expression was inhibited in glioma stem cells using small interference RNA (siRNAs) to examine its role in cell proliferation and tumorsphere formation. Bioinformatics analyses were also employed to investigate the association of SPRY1 expression with patient survival, tumor grade, and subtypes publicly available datasets. We demonstrated that SPRY1 is highly expressed in glioma stem cells than in NHA, glioma cells, and differentiated glioma stem cells. siRNA-mediated downregulation of SPRY1 expression decreased the stemness and self-renewal ability in GSC11. Bioinformatics results showed that high SPRY1 expression correlates with poor overall survival in glioma patients. Our findings suggest that SPRY1 contributes to the stemness and aggressiveness of GBM. Elsevier 2022-07-21 /pmc/articles/PMC9334334/ /pubmed/35910677 http://dx.doi.org/10.1016/j.ibneur.2022.07.003 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Paper
Park, Seo-Young
Jeong, Hang Yeon
Batara, Don Carlo
Lee, Suk Jun
Cho, Jeong-Yong
Kim, Sung-Hak
Sprouty 1 is associated with stemness and cancer progression in glioblastoma
title Sprouty 1 is associated with stemness and cancer progression in glioblastoma
title_full Sprouty 1 is associated with stemness and cancer progression in glioblastoma
title_fullStr Sprouty 1 is associated with stemness and cancer progression in glioblastoma
title_full_unstemmed Sprouty 1 is associated with stemness and cancer progression in glioblastoma
title_short Sprouty 1 is associated with stemness and cancer progression in glioblastoma
title_sort sprouty 1 is associated with stemness and cancer progression in glioblastoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334334/
https://www.ncbi.nlm.nih.gov/pubmed/35910677
http://dx.doi.org/10.1016/j.ibneur.2022.07.003
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