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An imaging mass cytometry immunophenotyping panel for non-human primate tissues

It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while pres...

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Autores principales: Niewold, Paula, Ijsselsteijn, Marieke E., Verreck, Frank A. W., Ottenhoff, Tom H. M., Joosten, Simone A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334813/
https://www.ncbi.nlm.nih.gov/pubmed/35911721
http://dx.doi.org/10.3389/fimmu.2022.915157
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author Niewold, Paula
Ijsselsteijn, Marieke E.
Verreck, Frank A. W.
Ottenhoff, Tom H. M.
Joosten, Simone A.
author_facet Niewold, Paula
Ijsselsteijn, Marieke E.
Verreck, Frank A. W.
Ottenhoff, Tom H. M.
Joosten, Simone A.
author_sort Niewold, Paula
collection PubMed
description It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable tool to unravel spatial relationships between cells. As availability of human tissue collected over the course of disease or infection is limited, preclinical models are a valuable source of such material. Non-human primate models are used for translational research as their anatomy, physiology and immune system closely resemble those of humans due to close evolutionary proximity. Tissue from non-human primate studies is often preserved large archives encompassing a range of conditions and organs. However, knowledge on antibody clones suitable for FFPE tissue of non-human primate origin is very limited. Here, we present an imaging mass cytometry panel development pipeline which enables the selection and incorporation of antibodies for imaging of non-human primate tissue. This has resulted in an 18-marker backbone panel which enables visualization of a broad range of leukocyte subsets in rhesus and cynomolgus macaque tissues. This high-dimensional imaging mass cytometry panel can be used to increase our knowledge of cellular organization within tissues and its effect on outcome of disease.
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spelling pubmed-93348132022-07-30 An imaging mass cytometry immunophenotyping panel for non-human primate tissues Niewold, Paula Ijsselsteijn, Marieke E. Verreck, Frank A. W. Ottenhoff, Tom H. M. Joosten, Simone A. Front Immunol Immunology It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable tool to unravel spatial relationships between cells. As availability of human tissue collected over the course of disease or infection is limited, preclinical models are a valuable source of such material. Non-human primate models are used for translational research as their anatomy, physiology and immune system closely resemble those of humans due to close evolutionary proximity. Tissue from non-human primate studies is often preserved large archives encompassing a range of conditions and organs. However, knowledge on antibody clones suitable for FFPE tissue of non-human primate origin is very limited. Here, we present an imaging mass cytometry panel development pipeline which enables the selection and incorporation of antibodies for imaging of non-human primate tissue. This has resulted in an 18-marker backbone panel which enables visualization of a broad range of leukocyte subsets in rhesus and cynomolgus macaque tissues. This high-dimensional imaging mass cytometry panel can be used to increase our knowledge of cellular organization within tissues and its effect on outcome of disease. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9334813/ /pubmed/35911721 http://dx.doi.org/10.3389/fimmu.2022.915157 Text en Copyright © 2022 Niewold, Ijsselsteijn, Verreck, Ottenhoff and Joosten https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Niewold, Paula
Ijsselsteijn, Marieke E.
Verreck, Frank A. W.
Ottenhoff, Tom H. M.
Joosten, Simone A.
An imaging mass cytometry immunophenotyping panel for non-human primate tissues
title An imaging mass cytometry immunophenotyping panel for non-human primate tissues
title_full An imaging mass cytometry immunophenotyping panel for non-human primate tissues
title_fullStr An imaging mass cytometry immunophenotyping panel for non-human primate tissues
title_full_unstemmed An imaging mass cytometry immunophenotyping panel for non-human primate tissues
title_short An imaging mass cytometry immunophenotyping panel for non-human primate tissues
title_sort imaging mass cytometry immunophenotyping panel for non-human primate tissues
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334813/
https://www.ncbi.nlm.nih.gov/pubmed/35911721
http://dx.doi.org/10.3389/fimmu.2022.915157
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