Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification

BACGROUND: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories. OBJECTIVES: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2...

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Autores principales: Madhavan, Anitha, Sachu, Arun, Sethuraman, Nandini, Kumar, Anil, Vasudevapanicker, Jayalakshmi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ghana Medical Association 2021
Materias:
Special Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334958/
https://www.ncbi.nlm.nih.gov/pubmed/35957934
http://dx.doi.org/10.4314/gmj.v55i4.12
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author Madhavan, Anitha
Sachu, Arun
Sethuraman, Nandini
Kumar, Anil
Vasudevapanicker, Jayalakshmi
author_facet Madhavan, Anitha
Sachu, Arun
Sethuraman, Nandini
Kumar, Anil
Vasudevapanicker, Jayalakshmi
author_sort Madhavan, Anitha
collection PubMed
description BACGROUND: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories. OBJECTIVES: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification. METHODS: The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms. RESULTS: MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level. CONCLUSION: MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology. FUNDING: State Board of Medical Research (SBMR).
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spelling pubmed-93349582022-08-10 Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification Madhavan, Anitha Sachu, Arun Sethuraman, Nandini Kumar, Anil Vasudevapanicker, Jayalakshmi Ghana Med J Special Article BACGROUND: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories. OBJECTIVES: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification. METHODS: The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms. RESULTS: MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level. CONCLUSION: MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology. FUNDING: State Board of Medical Research (SBMR). Ghana Medical Association 2021-12 /pmc/articles/PMC9334958/ /pubmed/35957934 http://dx.doi.org/10.4314/gmj.v55i4.12 Text en Copyright © The Author(s). https://creativecommons.org/licenses/by/4.0/This is an Open Access article under the CC BY license.
spellingShingle Special Article
Madhavan, Anitha
Sachu, Arun
Sethuraman, Nandini
Kumar, Anil
Vasudevapanicker, Jayalakshmi
Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification
title Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification
title_full Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification
title_fullStr Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification
title_full_unstemmed Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification
title_short Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification
title_sort evaluation of matrix-assisted laser desorption/ionization time-of flight mass spectrometry (maldi tof ms) and vitek 2 in routine microbial identification
topic Special Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334958/
https://www.ncbi.nlm.nih.gov/pubmed/35957934
http://dx.doi.org/10.4314/gmj.v55i4.12
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