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Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs

Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world, and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 pandemic. Here, we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding mult...

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Autores principales: Wang, Busen, Xu, Jinghan, Wu, Shipo, Zhang, Zhe, Zhao, Zhenghao, Zhang, Jun, Fu, Ling, Zai, Xiaodong, Wang, Yudong, Zhang, Guanying, Chen, ZhengShan, Chen, Yi, Sun, Hancong, Song, Xiaohong, Zhang, Jinlong, Zhu, Lianhui, Hou, Lihua, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334975/
https://www.ncbi.nlm.nih.gov/pubmed/35906201
http://dx.doi.org/10.1038/s41392-022-01065-0
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author Wang, Busen
Xu, Jinghan
Wu, Shipo
Zhang, Zhe
Zhao, Zhenghao
Zhang, Jun
Fu, Ling
Zai, Xiaodong
Wang, Yudong
Zhang, Guanying
Chen, ZhengShan
Chen, Yi
Sun, Hancong
Song, Xiaohong
Zhang, Jinlong
Zhu, Lianhui
Hou, Lihua
Chen, Wei
author_facet Wang, Busen
Xu, Jinghan
Wu, Shipo
Zhang, Zhe
Zhao, Zhenghao
Zhang, Jun
Fu, Ling
Zai, Xiaodong
Wang, Yudong
Zhang, Guanying
Chen, ZhengShan
Chen, Yi
Sun, Hancong
Song, Xiaohong
Zhang, Jinlong
Zhu, Lianhui
Hou, Lihua
Chen, Wei
author_sort Wang, Busen
collection PubMed
description Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world, and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 pandemic. Here, we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding multiple spike proteins derived from the Alpha, Beta, Gamma, Epsilon, Kappa, Delta and Omicron lineages and analyzed the antibody immune responses induced by single-dose and prime-boost vaccination strategies against emerging SARS-CoV-2 variants of concern (VOCs). Single-dose vaccination with SARS-CoV-2 variant vaccines tended to elicit the optimal self-matched neutralizing effects, and Ad5-B.1.351 produced more broad-spectrum cross-neutralizing antibodies against diverse variants. In contrast, prime-boost vaccination further strengthened and broadened the neutralizing antibody responses against highly divergent SARS-CoV-2 variants. The heterologous administration of Ad5-B.1.617.2 and Ad5-B.1.429 to Ad5-WT-primed mice resulted in superior antibody responses against most VOCs. In particular, the Omicron spike could only stimulate self-matched neutralizing antibodies with infrequent cross-reactivities to other variants used in single-dose vaccination strategies; moreover, with prime-boost regimens, this vaccine elicited an optimal specific neutralizing antibody response to Omicron, and prompted cross-antibody responses against other VOCs that were very similar to those obtained with Ad5-WT booster. Overall, this study delineated the unique characteristics of antibody responses to the SARS-CoV-2 VOC spikes with the single-dose or prime-boost vaccination strategies and provided insight into the vaccine development of next SARS-CoV-2 VOCs.
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spelling pubmed-93349752022-07-29 Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs Wang, Busen Xu, Jinghan Wu, Shipo Zhang, Zhe Zhao, Zhenghao Zhang, Jun Fu, Ling Zai, Xiaodong Wang, Yudong Zhang, Guanying Chen, ZhengShan Chen, Yi Sun, Hancong Song, Xiaohong Zhang, Jinlong Zhu, Lianhui Hou, Lihua Chen, Wei Signal Transduct Target Ther Article Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world, and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 pandemic. Here, we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding multiple spike proteins derived from the Alpha, Beta, Gamma, Epsilon, Kappa, Delta and Omicron lineages and analyzed the antibody immune responses induced by single-dose and prime-boost vaccination strategies against emerging SARS-CoV-2 variants of concern (VOCs). Single-dose vaccination with SARS-CoV-2 variant vaccines tended to elicit the optimal self-matched neutralizing effects, and Ad5-B.1.351 produced more broad-spectrum cross-neutralizing antibodies against diverse variants. In contrast, prime-boost vaccination further strengthened and broadened the neutralizing antibody responses against highly divergent SARS-CoV-2 variants. The heterologous administration of Ad5-B.1.617.2 and Ad5-B.1.429 to Ad5-WT-primed mice resulted in superior antibody responses against most VOCs. In particular, the Omicron spike could only stimulate self-matched neutralizing antibodies with infrequent cross-reactivities to other variants used in single-dose vaccination strategies; moreover, with prime-boost regimens, this vaccine elicited an optimal specific neutralizing antibody response to Omicron, and prompted cross-antibody responses against other VOCs that were very similar to those obtained with Ad5-WT booster. Overall, this study delineated the unique characteristics of antibody responses to the SARS-CoV-2 VOC spikes with the single-dose or prime-boost vaccination strategies and provided insight into the vaccine development of next SARS-CoV-2 VOCs. Nature Publishing Group UK 2022-07-29 /pmc/articles/PMC9334975/ /pubmed/35906201 http://dx.doi.org/10.1038/s41392-022-01065-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Busen
Xu, Jinghan
Wu, Shipo
Zhang, Zhe
Zhao, Zhenghao
Zhang, Jun
Fu, Ling
Zai, Xiaodong
Wang, Yudong
Zhang, Guanying
Chen, ZhengShan
Chen, Yi
Sun, Hancong
Song, Xiaohong
Zhang, Jinlong
Zhu, Lianhui
Hou, Lihua
Chen, Wei
Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs
title Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs
title_full Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs
title_fullStr Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs
title_full_unstemmed Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs
title_short Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs
title_sort comparative characterization of antibody responses induced by ad5-vectored spike proteins of emerging sars-cov-2 vocs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334975/
https://www.ncbi.nlm.nih.gov/pubmed/35906201
http://dx.doi.org/10.1038/s41392-022-01065-0
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