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Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1

Carotenoids, indispensable isoprenoid phytonutrients, are synthesized in plastids and are known to be deficient in rice endosperm. Many studies, involving transgenic manipulations of carotenoid biosynthetic genes, have been performed to obtain carotenoid-enriched rice grains. Nuclear-encoded GOLDEN2...

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Autores principales: Li, Zhenjun, Gao, Jianjie, Wang, Bo, Xu, Jing, Fu, Xiaoyan, Han, Hongjuan, Wang, Lijuan, Zhang, Wenhui, Deng, Yongdong, Wang, Yu, Gong, Zehao, Tian, Yongsheng, Peng, Rihe, Yao, Quanhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335051/
https://www.ncbi.nlm.nih.gov/pubmed/35909772
http://dx.doi.org/10.3389/fpls.2022.951605
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author Li, Zhenjun
Gao, Jianjie
Wang, Bo
Xu, Jing
Fu, Xiaoyan
Han, Hongjuan
Wang, Lijuan
Zhang, Wenhui
Deng, Yongdong
Wang, Yu
Gong, Zehao
Tian, Yongsheng
Peng, Rihe
Yao, Quanhong
author_facet Li, Zhenjun
Gao, Jianjie
Wang, Bo
Xu, Jing
Fu, Xiaoyan
Han, Hongjuan
Wang, Lijuan
Zhang, Wenhui
Deng, Yongdong
Wang, Yu
Gong, Zehao
Tian, Yongsheng
Peng, Rihe
Yao, Quanhong
author_sort Li, Zhenjun
collection PubMed
description Carotenoids, indispensable isoprenoid phytonutrients, are synthesized in plastids and are known to be deficient in rice endosperm. Many studies, involving transgenic manipulations of carotenoid biosynthetic genes, have been performed to obtain carotenoid-enriched rice grains. Nuclear-encoded GOLDEN2-LIKE (GLK) transcription factors play important roles in the regulation of plastid and thylakoid grana development. Here, we show that endosperm-specific overexpression of rice GLK1 gene (OsGLK1) leads to enhanced carotenoid production, increased grain yield, but deteriorated grain quality in rice. Subsequently, we performed the bioengineering of carotenoids biosynthesis in rice endosperm by introducing other three carotenogenic genes, tHMG1, ZmPSY1, and PaCrtI, which encode the enzymes truncated 3-hydroxy-3-methylglutaryl-CoA reductase, phytoene synthase, and phytoene desaturase, respectively. Transgenic overexpression of all four genes (OsGLK1, tHMG1, ZmPSY1, and PaCrtI) driven by rice endosperm-specific promoter GluB-1 established a mini carotenoid biosynthetic pathway in the endosperm and exerted a roughly multiplicative effect on the carotenoid accumulation as compared with the overexpression of only three genes (tHMG1, ZmPSY1, and PaCrtI). In addition, the yield enhancement and quality reduction traits were also present in the transgenic rice overexpressing the selected four genes. Our results revealed that OsGLK1 confers favorable characters in rice endosperm and could help to refine strategies for the carotenoid and other plastid-synthesized micronutrient fortification in bioengineered plants.
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spelling pubmed-93350512022-07-30 Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1 Li, Zhenjun Gao, Jianjie Wang, Bo Xu, Jing Fu, Xiaoyan Han, Hongjuan Wang, Lijuan Zhang, Wenhui Deng, Yongdong Wang, Yu Gong, Zehao Tian, Yongsheng Peng, Rihe Yao, Quanhong Front Plant Sci Plant Science Carotenoids, indispensable isoprenoid phytonutrients, are synthesized in plastids and are known to be deficient in rice endosperm. Many studies, involving transgenic manipulations of carotenoid biosynthetic genes, have been performed to obtain carotenoid-enriched rice grains. Nuclear-encoded GOLDEN2-LIKE (GLK) transcription factors play important roles in the regulation of plastid and thylakoid grana development. Here, we show that endosperm-specific overexpression of rice GLK1 gene (OsGLK1) leads to enhanced carotenoid production, increased grain yield, but deteriorated grain quality in rice. Subsequently, we performed the bioengineering of carotenoids biosynthesis in rice endosperm by introducing other three carotenogenic genes, tHMG1, ZmPSY1, and PaCrtI, which encode the enzymes truncated 3-hydroxy-3-methylglutaryl-CoA reductase, phytoene synthase, and phytoene desaturase, respectively. Transgenic overexpression of all four genes (OsGLK1, tHMG1, ZmPSY1, and PaCrtI) driven by rice endosperm-specific promoter GluB-1 established a mini carotenoid biosynthetic pathway in the endosperm and exerted a roughly multiplicative effect on the carotenoid accumulation as compared with the overexpression of only three genes (tHMG1, ZmPSY1, and PaCrtI). In addition, the yield enhancement and quality reduction traits were also present in the transgenic rice overexpressing the selected four genes. Our results revealed that OsGLK1 confers favorable characters in rice endosperm and could help to refine strategies for the carotenoid and other plastid-synthesized micronutrient fortification in bioengineered plants. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9335051/ /pubmed/35909772 http://dx.doi.org/10.3389/fpls.2022.951605 Text en Copyright © 2022 Li, Gao, Wang, Xu, Fu, Han, Wang, Zhang, Deng, Wang, Gong, Tian, Peng and Yao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Zhenjun
Gao, Jianjie
Wang, Bo
Xu, Jing
Fu, Xiaoyan
Han, Hongjuan
Wang, Lijuan
Zhang, Wenhui
Deng, Yongdong
Wang, Yu
Gong, Zehao
Tian, Yongsheng
Peng, Rihe
Yao, Quanhong
Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1
title Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1
title_full Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1
title_fullStr Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1
title_full_unstemmed Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1
title_short Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1
title_sort rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of osglk1
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335051/
https://www.ncbi.nlm.nih.gov/pubmed/35909772
http://dx.doi.org/10.3389/fpls.2022.951605
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