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Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus

Amino acids could act as nitrogen sources, amido group donors, or bioactive molecules in fungi fermentation, and consequently, play important roles in Monascus pigments (MPs) biosynthesis. But the understanding of the effects of various amino acids on MPs biosynthesis is still incomprehensive. In th...

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Autores principales: Yin, Sheng, Zhu, Yiying, Zhang, Bin, Huang, Baozhu, Jia, Ru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335072/
https://www.ncbi.nlm.nih.gov/pubmed/35910612
http://dx.doi.org/10.3389/fmicb.2022.951266
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author Yin, Sheng
Zhu, Yiying
Zhang, Bin
Huang, Baozhu
Jia, Ru
author_facet Yin, Sheng
Zhu, Yiying
Zhang, Bin
Huang, Baozhu
Jia, Ru
author_sort Yin, Sheng
collection PubMed
description Amino acids could act as nitrogen sources, amido group donors, or bioactive molecules in fungi fermentation, and consequently, play important roles in Monascus pigments (MPs) biosynthesis. But the understanding of the effects of various amino acids on MPs biosynthesis is still incomprehensive. In this work, 20 free amino acids were added to the fermentation medium to evaluate their effects on MPs biosynthesis in Monascus purpureus RP2. Six amino acids, namely, histidine (HIS), lysine (LYS), tyrosine (TYR), phenylalanine (PHE), methionine (MET), and cysteine (CYS), were selected as the valuable ones as they exerted significant effects on the production yield and even on the biosynthesis metabolic curves of MPs. Moreover, the dose-dependent and synergistic effects of valuable amino acids on MPs biosynthesis were observed by tests of serial concentrations and different combinations. In addition, it revealed that HIS and MET were the prominent amino acids with dominant and universal influences on MPs biosynthesis. The analog compounds of HIS (amitrole) and MET [calcium 2-hydroxy-4-(methylthio)] were added to the fermentation medium, and the results further confirmed the extraordinary effects of HIS and MET and their analogs on MPs biosynthesis. Furthermore, the gene transcription profile indicated that a differential expression pattern was observed in the polyketide synthase (PKS) cluster responsible for MPs biosynthesis in response to HIS and MET, revealing that they could oppositely regulate MPs biosynthesis in different ways. These findings would benefit the understanding of MPs biosynthesis regulation mechanism in M. purpureus and contribute to the industrial production of MPs by fermentation.
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spelling pubmed-93350722022-07-30 Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus Yin, Sheng Zhu, Yiying Zhang, Bin Huang, Baozhu Jia, Ru Front Microbiol Microbiology Amino acids could act as nitrogen sources, amido group donors, or bioactive molecules in fungi fermentation, and consequently, play important roles in Monascus pigments (MPs) biosynthesis. But the understanding of the effects of various amino acids on MPs biosynthesis is still incomprehensive. In this work, 20 free amino acids were added to the fermentation medium to evaluate their effects on MPs biosynthesis in Monascus purpureus RP2. Six amino acids, namely, histidine (HIS), lysine (LYS), tyrosine (TYR), phenylalanine (PHE), methionine (MET), and cysteine (CYS), were selected as the valuable ones as they exerted significant effects on the production yield and even on the biosynthesis metabolic curves of MPs. Moreover, the dose-dependent and synergistic effects of valuable amino acids on MPs biosynthesis were observed by tests of serial concentrations and different combinations. In addition, it revealed that HIS and MET were the prominent amino acids with dominant and universal influences on MPs biosynthesis. The analog compounds of HIS (amitrole) and MET [calcium 2-hydroxy-4-(methylthio)] were added to the fermentation medium, and the results further confirmed the extraordinary effects of HIS and MET and their analogs on MPs biosynthesis. Furthermore, the gene transcription profile indicated that a differential expression pattern was observed in the polyketide synthase (PKS) cluster responsible for MPs biosynthesis in response to HIS and MET, revealing that they could oppositely regulate MPs biosynthesis in different ways. These findings would benefit the understanding of MPs biosynthesis regulation mechanism in M. purpureus and contribute to the industrial production of MPs by fermentation. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9335072/ /pubmed/35910612 http://dx.doi.org/10.3389/fmicb.2022.951266 Text en Copyright © 2022 Yin, Zhu, Zhang, Huang and Jia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yin, Sheng
Zhu, Yiying
Zhang, Bin
Huang, Baozhu
Jia, Ru
Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
title Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
title_full Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
title_fullStr Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
title_full_unstemmed Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
title_short Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
title_sort diverse effects of amino acids on monascus pigments biosynthesis in monascus purpureus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335072/
https://www.ncbi.nlm.nih.gov/pubmed/35910612
http://dx.doi.org/10.3389/fmicb.2022.951266
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