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Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus
Amino acids could act as nitrogen sources, amido group donors, or bioactive molecules in fungi fermentation, and consequently, play important roles in Monascus pigments (MPs) biosynthesis. But the understanding of the effects of various amino acids on MPs biosynthesis is still incomprehensive. In th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335072/ https://www.ncbi.nlm.nih.gov/pubmed/35910612 http://dx.doi.org/10.3389/fmicb.2022.951266 |
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author | Yin, Sheng Zhu, Yiying Zhang, Bin Huang, Baozhu Jia, Ru |
author_facet | Yin, Sheng Zhu, Yiying Zhang, Bin Huang, Baozhu Jia, Ru |
author_sort | Yin, Sheng |
collection | PubMed |
description | Amino acids could act as nitrogen sources, amido group donors, or bioactive molecules in fungi fermentation, and consequently, play important roles in Monascus pigments (MPs) biosynthesis. But the understanding of the effects of various amino acids on MPs biosynthesis is still incomprehensive. In this work, 20 free amino acids were added to the fermentation medium to evaluate their effects on MPs biosynthesis in Monascus purpureus RP2. Six amino acids, namely, histidine (HIS), lysine (LYS), tyrosine (TYR), phenylalanine (PHE), methionine (MET), and cysteine (CYS), were selected as the valuable ones as they exerted significant effects on the production yield and even on the biosynthesis metabolic curves of MPs. Moreover, the dose-dependent and synergistic effects of valuable amino acids on MPs biosynthesis were observed by tests of serial concentrations and different combinations. In addition, it revealed that HIS and MET were the prominent amino acids with dominant and universal influences on MPs biosynthesis. The analog compounds of HIS (amitrole) and MET [calcium 2-hydroxy-4-(methylthio)] were added to the fermentation medium, and the results further confirmed the extraordinary effects of HIS and MET and their analogs on MPs biosynthesis. Furthermore, the gene transcription profile indicated that a differential expression pattern was observed in the polyketide synthase (PKS) cluster responsible for MPs biosynthesis in response to HIS and MET, revealing that they could oppositely regulate MPs biosynthesis in different ways. These findings would benefit the understanding of MPs biosynthesis regulation mechanism in M. purpureus and contribute to the industrial production of MPs by fermentation. |
format | Online Article Text |
id | pubmed-9335072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93350722022-07-30 Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus Yin, Sheng Zhu, Yiying Zhang, Bin Huang, Baozhu Jia, Ru Front Microbiol Microbiology Amino acids could act as nitrogen sources, amido group donors, or bioactive molecules in fungi fermentation, and consequently, play important roles in Monascus pigments (MPs) biosynthesis. But the understanding of the effects of various amino acids on MPs biosynthesis is still incomprehensive. In this work, 20 free amino acids were added to the fermentation medium to evaluate their effects on MPs biosynthesis in Monascus purpureus RP2. Six amino acids, namely, histidine (HIS), lysine (LYS), tyrosine (TYR), phenylalanine (PHE), methionine (MET), and cysteine (CYS), were selected as the valuable ones as they exerted significant effects on the production yield and even on the biosynthesis metabolic curves of MPs. Moreover, the dose-dependent and synergistic effects of valuable amino acids on MPs biosynthesis were observed by tests of serial concentrations and different combinations. In addition, it revealed that HIS and MET were the prominent amino acids with dominant and universal influences on MPs biosynthesis. The analog compounds of HIS (amitrole) and MET [calcium 2-hydroxy-4-(methylthio)] were added to the fermentation medium, and the results further confirmed the extraordinary effects of HIS and MET and their analogs on MPs biosynthesis. Furthermore, the gene transcription profile indicated that a differential expression pattern was observed in the polyketide synthase (PKS) cluster responsible for MPs biosynthesis in response to HIS and MET, revealing that they could oppositely regulate MPs biosynthesis in different ways. These findings would benefit the understanding of MPs biosynthesis regulation mechanism in M. purpureus and contribute to the industrial production of MPs by fermentation. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9335072/ /pubmed/35910612 http://dx.doi.org/10.3389/fmicb.2022.951266 Text en Copyright © 2022 Yin, Zhu, Zhang, Huang and Jia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yin, Sheng Zhu, Yiying Zhang, Bin Huang, Baozhu Jia, Ru Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus |
title | Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus |
title_full | Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus |
title_fullStr | Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus |
title_full_unstemmed | Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus |
title_short | Diverse Effects of Amino Acids on Monascus Pigments Biosynthesis in Monascus purpureus |
title_sort | diverse effects of amino acids on monascus pigments biosynthesis in monascus purpureus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335072/ https://www.ncbi.nlm.nih.gov/pubmed/35910612 http://dx.doi.org/10.3389/fmicb.2022.951266 |
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