Cargando…
Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae
Tea (Camellia sinensis) is an important crop that is mainly used in the food industry. This study using the metabolome and microbiome investigates the resistance factors of wild tea plant resources against tea gray blight disease, which is caused by Pestalotiopsis theae (Sawada) Steyaert. According...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335280/ https://www.ncbi.nlm.nih.gov/pubmed/35910661 http://dx.doi.org/10.3389/fmicb.2022.907962 |
_version_ | 1784759302027739136 |
---|---|
author | Zhang, Yuqian Zhang, Jie Yan, Changyu Fang, Meishan Wang, Lijie Huang, Yahui Wang, Feiyan |
author_facet | Zhang, Yuqian Zhang, Jie Yan, Changyu Fang, Meishan Wang, Lijie Huang, Yahui Wang, Feiyan |
author_sort | Zhang, Yuqian |
collection | PubMed |
description | Tea (Camellia sinensis) is an important crop that is mainly used in the food industry. This study using the metabolome and microbiome investigates the resistance factors of wild tea plant resources against tea gray blight disease, which is caused by Pestalotiopsis theae (Sawada) Steyaert. According to the interaction analysis of tea leaves and pathogenic fungus, the resistance of wild tea plant resource “R1” (Resistance 1) to tea gray blight disease was significantly higher than that of wild tea plant resource “S1” (Susceptibility 1). The difference between “R1” and “S1” in the metabolome was obvious. There were 145 metabolites that significantly changed. The phenolic acids and flavonoids were the major increased categories in “R1,” and it included 4-O-glucosyl-sinapate and petunidin-3-o-(6”-o-p-coumaroyl) rutinoside. Six metabolic pathways were significantly enriched, including aminoacyl-tRNA biosynthesis, flavone, and flavonol biosynthesis. In terms of bacteria, there was no significant difference between “S1” and “R1” in the principal component analysis (PCA). Pseudomonas was the major bacterial genus in “S1” and “R1.” In addition, each of the two resources had its own predominant genus: Cellvibirio was a predominant bacterial genus in “S1” and Candidatus_competibacter was a predominant bacterial genus in “R1.” In terms of fungi, the fungal diversity and the abundance of the two tea plant resource samples could be distinguished clearly. The fungal component of “S1” was more abundant than that of “R1” at the genus level. Toxicocladosporium was the predominant fungal genus of “S1,” and Filobasidium was the predominant fungal genus of “R1.” The relative abundance of unclassified-norank-norank-Chloroplast and Penicillium were significantly different between “S1” and “R1.” Penicillium was identified as a potential biomarker. They correlated with some metabolites enriched in “S1” or “R1,” such as L-arginine and quercetin-3-o-(2”-o-rhamnosyl) rutinoside-7-o-glucoside. Overall, phenolic acids, flavonoids, and Penicillium could be functional metabolites or microorganisms that contributed to improving the resistance of wild tea plant resources to tea gray blight disease. |
format | Online Article Text |
id | pubmed-9335280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93352802022-07-30 Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae Zhang, Yuqian Zhang, Jie Yan, Changyu Fang, Meishan Wang, Lijie Huang, Yahui Wang, Feiyan Front Microbiol Microbiology Tea (Camellia sinensis) is an important crop that is mainly used in the food industry. This study using the metabolome and microbiome investigates the resistance factors of wild tea plant resources against tea gray blight disease, which is caused by Pestalotiopsis theae (Sawada) Steyaert. According to the interaction analysis of tea leaves and pathogenic fungus, the resistance of wild tea plant resource “R1” (Resistance 1) to tea gray blight disease was significantly higher than that of wild tea plant resource “S1” (Susceptibility 1). The difference between “R1” and “S1” in the metabolome was obvious. There were 145 metabolites that significantly changed. The phenolic acids and flavonoids were the major increased categories in “R1,” and it included 4-O-glucosyl-sinapate and petunidin-3-o-(6”-o-p-coumaroyl) rutinoside. Six metabolic pathways were significantly enriched, including aminoacyl-tRNA biosynthesis, flavone, and flavonol biosynthesis. In terms of bacteria, there was no significant difference between “S1” and “R1” in the principal component analysis (PCA). Pseudomonas was the major bacterial genus in “S1” and “R1.” In addition, each of the two resources had its own predominant genus: Cellvibirio was a predominant bacterial genus in “S1” and Candidatus_competibacter was a predominant bacterial genus in “R1.” In terms of fungi, the fungal diversity and the abundance of the two tea plant resource samples could be distinguished clearly. The fungal component of “S1” was more abundant than that of “R1” at the genus level. Toxicocladosporium was the predominant fungal genus of “S1,” and Filobasidium was the predominant fungal genus of “R1.” The relative abundance of unclassified-norank-norank-Chloroplast and Penicillium were significantly different between “S1” and “R1.” Penicillium was identified as a potential biomarker. They correlated with some metabolites enriched in “S1” or “R1,” such as L-arginine and quercetin-3-o-(2”-o-rhamnosyl) rutinoside-7-o-glucoside. Overall, phenolic acids, flavonoids, and Penicillium could be functional metabolites or microorganisms that contributed to improving the resistance of wild tea plant resources to tea gray blight disease. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9335280/ /pubmed/35910661 http://dx.doi.org/10.3389/fmicb.2022.907962 Text en Copyright © 2022 Zhang, Zhang, Yan, Fang, Wang, Huang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Yuqian Zhang, Jie Yan, Changyu Fang, Meishan Wang, Lijie Huang, Yahui Wang, Feiyan Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae |
title | Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae |
title_full | Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae |
title_fullStr | Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae |
title_full_unstemmed | Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae |
title_short | Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae |
title_sort | metabolome and microbiome signatures in the leaves of wild tea plant resources resistant to pestalotiopsis theae |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335280/ https://www.ncbi.nlm.nih.gov/pubmed/35910661 http://dx.doi.org/10.3389/fmicb.2022.907962 |
work_keys_str_mv | AT zhangyuqian metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae AT zhangjie metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae AT yanchangyu metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae AT fangmeishan metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae AT wanglijie metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae AT huangyahui metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae AT wangfeiyan metabolomeandmicrobiomesignaturesintheleavesofwildteaplantresourcesresistanttopestalotiopsistheae |