Cargando…
Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens
With the increasing bacterial resistance to traditional antibiotics, there is an urgent need for the development of alternative drugs or adjuvants of antibiotics to enhance antibacterial efficiency. The combination of antimicrobial peptides (AMPs) and traditional antibiotics is a potential alternati...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335283/ https://www.ncbi.nlm.nih.gov/pubmed/35910608 http://dx.doi.org/10.3389/fmicb.2022.953720 |
_version_ | 1784759302885474304 |
---|---|
author | Lu, Yi Tian, Hongliang Chen, Runqiu Liu, Qian Jia, Kaixiang Hu, Dong-Liang Chen, Hongwei Ye, Chao Peng, Lianci Fang, Rendong |
author_facet | Lu, Yi Tian, Hongliang Chen, Runqiu Liu, Qian Jia, Kaixiang Hu, Dong-Liang Chen, Hongwei Ye, Chao Peng, Lianci Fang, Rendong |
author_sort | Lu, Yi |
collection | PubMed |
description | With the increasing bacterial resistance to traditional antibiotics, there is an urgent need for the development of alternative drugs or adjuvants of antibiotics to enhance antibacterial efficiency. The combination of antimicrobial peptides (AMPs) and traditional antibiotics is a potential alternative to enhance antibacterial efficiency. In this study, we investigated the synergistic bactericidal effect of AMPs, including chicken (CATH-1,−2,−3, and -B1), mice (CRAMP), and porcine (PMAP-36 and PR-39) in combination with conventional antibiotics containing ampicillin, tetracycline, gentamicin, and erythromycin against Staphylococcus aureus, Salmonella enteritidis, and Escherichia coli. The results showed that the minimum bactericidal concentration (MBC) of CATH-1,−3 and PMAP-36 was lower than 10 μM, indicating that these three AMPs had good bacterial activity against S. aureus, S. enteritidis, and E. coli. Then, the synergistic antibacterial activity of AMPs and antibiotics combination was determined by the fractional bactericidal concentration index (FBCI). The results showed that the FBCI of AMPs (CATH-1,−3 and PMAP-36) and erythromycin was lower than 0.5 against bacterial pathogens, demonstrating that they had a synergistic bactericidal effect. Furthermore, the time-killing kinetics of AMPs (CATH-1,−3 and PMAP-36) in combination with erythromycin showed that they had a continuous killing effect on bacteria within 3 h. Notably, the combination showed lower hemolytic activity and cytotoxicity to mammal cells compared to erythromycin and peptide alone treatment. In addition, the antibacterial mechanism of CATH-1 and erythromycin combination against E. coli was studied. The results of the scanning electron microscope showed that CATH-1 enhanced the antibacterial activity of erythromycin by increasing the permeability of bacterial cell membrane. Moreover, the results of bacterial migration movement showed that the combination of CATH-1 and erythromycin significantly inhibits the migration of E. coli. Finally, drug resistance analysis was performed and the results showed that CATH-1 delayed the emergence of E. coli resistance to erythromycin. In conclusion, the combination of CATH-1 and erythromycin has synergistic antibacterial activity and reduces the emergence of bacterial drug resistance. Our study provides valuable information to develop AMPs as potential substitutes or adjuvants for traditional antibiotics. |
format | Online Article Text |
id | pubmed-9335283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93352832022-07-30 Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens Lu, Yi Tian, Hongliang Chen, Runqiu Liu, Qian Jia, Kaixiang Hu, Dong-Liang Chen, Hongwei Ye, Chao Peng, Lianci Fang, Rendong Front Microbiol Microbiology With the increasing bacterial resistance to traditional antibiotics, there is an urgent need for the development of alternative drugs or adjuvants of antibiotics to enhance antibacterial efficiency. The combination of antimicrobial peptides (AMPs) and traditional antibiotics is a potential alternative to enhance antibacterial efficiency. In this study, we investigated the synergistic bactericidal effect of AMPs, including chicken (CATH-1,−2,−3, and -B1), mice (CRAMP), and porcine (PMAP-36 and PR-39) in combination with conventional antibiotics containing ampicillin, tetracycline, gentamicin, and erythromycin against Staphylococcus aureus, Salmonella enteritidis, and Escherichia coli. The results showed that the minimum bactericidal concentration (MBC) of CATH-1,−3 and PMAP-36 was lower than 10 μM, indicating that these three AMPs had good bacterial activity against S. aureus, S. enteritidis, and E. coli. Then, the synergistic antibacterial activity of AMPs and antibiotics combination was determined by the fractional bactericidal concentration index (FBCI). The results showed that the FBCI of AMPs (CATH-1,−3 and PMAP-36) and erythromycin was lower than 0.5 against bacterial pathogens, demonstrating that they had a synergistic bactericidal effect. Furthermore, the time-killing kinetics of AMPs (CATH-1,−3 and PMAP-36) in combination with erythromycin showed that they had a continuous killing effect on bacteria within 3 h. Notably, the combination showed lower hemolytic activity and cytotoxicity to mammal cells compared to erythromycin and peptide alone treatment. In addition, the antibacterial mechanism of CATH-1 and erythromycin combination against E. coli was studied. The results of the scanning electron microscope showed that CATH-1 enhanced the antibacterial activity of erythromycin by increasing the permeability of bacterial cell membrane. Moreover, the results of bacterial migration movement showed that the combination of CATH-1 and erythromycin significantly inhibits the migration of E. coli. Finally, drug resistance analysis was performed and the results showed that CATH-1 delayed the emergence of E. coli resistance to erythromycin. In conclusion, the combination of CATH-1 and erythromycin has synergistic antibacterial activity and reduces the emergence of bacterial drug resistance. Our study provides valuable information to develop AMPs as potential substitutes or adjuvants for traditional antibiotics. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9335283/ /pubmed/35910608 http://dx.doi.org/10.3389/fmicb.2022.953720 Text en Copyright © 2022 Lu, Tian, Chen, Liu, Jia, Hu, Chen, Ye, Peng and Fang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lu, Yi Tian, Hongliang Chen, Runqiu Liu, Qian Jia, Kaixiang Hu, Dong-Liang Chen, Hongwei Ye, Chao Peng, Lianci Fang, Rendong Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens |
title | Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens |
title_full | Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens |
title_fullStr | Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens |
title_full_unstemmed | Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens |
title_short | Synergistic Antimicrobial Effect of Antimicrobial Peptides CATH-1, CATH-3, and PMAP-36 With Erythromycin Against Bacterial Pathogens |
title_sort | synergistic antimicrobial effect of antimicrobial peptides cath-1, cath-3, and pmap-36 with erythromycin against bacterial pathogens |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335283/ https://www.ncbi.nlm.nih.gov/pubmed/35910608 http://dx.doi.org/10.3389/fmicb.2022.953720 |
work_keys_str_mv | AT luyi synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT tianhongliang synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT chenrunqiu synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT liuqian synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT jiakaixiang synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT hudongliang synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT chenhongwei synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT yechao synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT penglianci synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens AT fangrendong synergisticantimicrobialeffectofantimicrobialpeptidescath1cath3andpmap36witherythromycinagainstbacterialpathogens |