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A 3D-printed transepidermal microprojection array for human skin microbiome sampling
Skin microbiome sampling is currently performed with tools such as swabs and tape strips to collect microbes from the skin surface. However, these conventional approaches may be unable to detect microbes deeper in the epidermis or in epidermal invaginations. We describe a sampling tool with a depth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335308/ https://www.ncbi.nlm.nih.gov/pubmed/35867832 http://dx.doi.org/10.1073/pnas.2203556119 |
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author | Liang, Kun Leong, Cheryl Loh, Jia Min Chan, Nathania Lim, Larissa Lam, Yuen In Dawson, Thomas L. Tey, Hong Liang |
author_facet | Liang, Kun Leong, Cheryl Loh, Jia Min Chan, Nathania Lim, Larissa Lam, Yuen In Dawson, Thomas L. Tey, Hong Liang |
author_sort | Liang, Kun |
collection | PubMed |
description | Skin microbiome sampling is currently performed with tools such as swabs and tape strips to collect microbes from the skin surface. However, these conventional approaches may be unable to detect microbes deeper in the epidermis or in epidermal invaginations. We describe a sampling tool with a depth component, a transepidermal microprojection array (MPA), which captures microbial biomass from both the epidermal surface and deeper skin layers. We leveraged the rapid customizability of 3D printing to enable systematic optimization of MPA for human skin sampling. Evaluation of sampling efficacy on human scalp revealed the optimized MPA was comparable in sensitivity to swab and superior to tape strip, especially for nonstandard skin surfaces. We observed differences in species diversity, with the MPA detecting clinically relevant fungi more often than other approaches. This work delivers a tool in the complex field of skin microbiome sampling to potentially address gaps in our understanding of its role in health and disease. |
format | Online Article Text |
id | pubmed-9335308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-93353082023-01-22 A 3D-printed transepidermal microprojection array for human skin microbiome sampling Liang, Kun Leong, Cheryl Loh, Jia Min Chan, Nathania Lim, Larissa Lam, Yuen In Dawson, Thomas L. Tey, Hong Liang Proc Natl Acad Sci U S A Physical Sciences Skin microbiome sampling is currently performed with tools such as swabs and tape strips to collect microbes from the skin surface. However, these conventional approaches may be unable to detect microbes deeper in the epidermis or in epidermal invaginations. We describe a sampling tool with a depth component, a transepidermal microprojection array (MPA), which captures microbial biomass from both the epidermal surface and deeper skin layers. We leveraged the rapid customizability of 3D printing to enable systematic optimization of MPA for human skin sampling. Evaluation of sampling efficacy on human scalp revealed the optimized MPA was comparable in sensitivity to swab and superior to tape strip, especially for nonstandard skin surfaces. We observed differences in species diversity, with the MPA detecting clinically relevant fungi more often than other approaches. This work delivers a tool in the complex field of skin microbiome sampling to potentially address gaps in our understanding of its role in health and disease. National Academy of Sciences 2022-07-22 2022-07-26 /pmc/articles/PMC9335308/ /pubmed/35867832 http://dx.doi.org/10.1073/pnas.2203556119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Physical Sciences Liang, Kun Leong, Cheryl Loh, Jia Min Chan, Nathania Lim, Larissa Lam, Yuen In Dawson, Thomas L. Tey, Hong Liang A 3D-printed transepidermal microprojection array for human skin microbiome sampling |
title | A 3D-printed transepidermal microprojection array for human skin microbiome sampling |
title_full | A 3D-printed transepidermal microprojection array for human skin microbiome sampling |
title_fullStr | A 3D-printed transepidermal microprojection array for human skin microbiome sampling |
title_full_unstemmed | A 3D-printed transepidermal microprojection array for human skin microbiome sampling |
title_short | A 3D-printed transepidermal microprojection array for human skin microbiome sampling |
title_sort | 3d-printed transepidermal microprojection array for human skin microbiome sampling |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335308/ https://www.ncbi.nlm.nih.gov/pubmed/35867832 http://dx.doi.org/10.1073/pnas.2203556119 |
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