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Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions

NF-κB–mediated endothelial activation drives leukocyte recruitment and atherosclerosis, in part through adhesion molecules Icam1 and Vcam1. The endothelium is primed for cytokine activation of NF-κB by exposure to low and disturbed blood flow (LDF)but the molecular underpinnings are not fully unders...

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Autores principales: Hensel, Jessica A., Nicholas, Sarah-Anne E., Kimble, Amy L., Nagpal, Arjun S., Omar, Omar M. F., Tyburski, Jordan D., Jellison, Evan R., Ménoret, Antoine, Ozawa, Manabu, Rodriguez-Oquendo, Annabelle, Vella, Anthony T., Murphy, Patrick A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335344/
https://www.ncbi.nlm.nih.gov/pubmed/35858420
http://dx.doi.org/10.1073/pnas.2122227119
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author Hensel, Jessica A.
Nicholas, Sarah-Anne E.
Kimble, Amy L.
Nagpal, Arjun S.
Omar, Omar M. F.
Tyburski, Jordan D.
Jellison, Evan R.
Ménoret, Antoine
Ozawa, Manabu
Rodriguez-Oquendo, Annabelle
Vella, Anthony T.
Murphy, Patrick A.
author_facet Hensel, Jessica A.
Nicholas, Sarah-Anne E.
Kimble, Amy L.
Nagpal, Arjun S.
Omar, Omar M. F.
Tyburski, Jordan D.
Jellison, Evan R.
Ménoret, Antoine
Ozawa, Manabu
Rodriguez-Oquendo, Annabelle
Vella, Anthony T.
Murphy, Patrick A.
author_sort Hensel, Jessica A.
collection PubMed
description NF-κB–mediated endothelial activation drives leukocyte recruitment and atherosclerosis, in part through adhesion molecules Icam1 and Vcam1. The endothelium is primed for cytokine activation of NF-κB by exposure to low and disturbed blood flow (LDF)but the molecular underpinnings are not fully understood. In an experimental in vivo model of LDF, platelets were required for the increased expression of several RNA-binding splice factors, including polypyrimidine tract binding protein (Ptbp1). This was coordinated with changes in RNA splicing in the NF-κB pathway in primed cells, leading us to examine splice factors as mediators of priming. Using Icam1 and Vcam1 induction by tumor necrosis factor (TNF)-α stimulation as a readout, we performed a CRISPR Cas9 knockout screen and identified a requirement for Ptbp1 in priming. Deletion of Ptbp1 had no effect on cell growth or response to apoptotic stimuli, but reversed LDF splicing patterns and inhibited NF-κB nuclear translocation and transcriptional activation of downstream targets, including Icam1 and Vcam1. In human coronary arteries, elevated PTBP1 correlates with expression of TNF pathway genes and plaque. In vivo, endothelial-specific deletion of Ptbp1 reduced Icam1 expression and myeloid cell infiltration at regions of LDF in atherosclerotic mice, limiting atherosclerosis. This may be mediated, in part, by allowing inclusion of a conserved alternative exon in Ripk1 leading to a reduction in Ripk1 protein. Our data show that Ptbp1, which is induced in a subset of the endothelium by platelet recruitment at regions of LDF, is required for priming of the endothelium for subsequent NF-κB activation, myeloid cell recruitment and atherosclerosis.
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spelling pubmed-93353442022-07-30 Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions Hensel, Jessica A. Nicholas, Sarah-Anne E. Kimble, Amy L. Nagpal, Arjun S. Omar, Omar M. F. Tyburski, Jordan D. Jellison, Evan R. Ménoret, Antoine Ozawa, Manabu Rodriguez-Oquendo, Annabelle Vella, Anthony T. Murphy, Patrick A. Proc Natl Acad Sci U S A Biological Sciences NF-κB–mediated endothelial activation drives leukocyte recruitment and atherosclerosis, in part through adhesion molecules Icam1 and Vcam1. The endothelium is primed for cytokine activation of NF-κB by exposure to low and disturbed blood flow (LDF)but the molecular underpinnings are not fully understood. In an experimental in vivo model of LDF, platelets were required for the increased expression of several RNA-binding splice factors, including polypyrimidine tract binding protein (Ptbp1). This was coordinated with changes in RNA splicing in the NF-κB pathway in primed cells, leading us to examine splice factors as mediators of priming. Using Icam1 and Vcam1 induction by tumor necrosis factor (TNF)-α stimulation as a readout, we performed a CRISPR Cas9 knockout screen and identified a requirement for Ptbp1 in priming. Deletion of Ptbp1 had no effect on cell growth or response to apoptotic stimuli, but reversed LDF splicing patterns and inhibited NF-κB nuclear translocation and transcriptional activation of downstream targets, including Icam1 and Vcam1. In human coronary arteries, elevated PTBP1 correlates with expression of TNF pathway genes and plaque. In vivo, endothelial-specific deletion of Ptbp1 reduced Icam1 expression and myeloid cell infiltration at regions of LDF in atherosclerotic mice, limiting atherosclerosis. This may be mediated, in part, by allowing inclusion of a conserved alternative exon in Ripk1 leading to a reduction in Ripk1 protein. Our data show that Ptbp1, which is induced in a subset of the endothelium by platelet recruitment at regions of LDF, is required for priming of the endothelium for subsequent NF-κB activation, myeloid cell recruitment and atherosclerosis. National Academy of Sciences 2022-07-18 2022-07-26 /pmc/articles/PMC9335344/ /pubmed/35858420 http://dx.doi.org/10.1073/pnas.2122227119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Hensel, Jessica A.
Nicholas, Sarah-Anne E.
Kimble, Amy L.
Nagpal, Arjun S.
Omar, Omar M. F.
Tyburski, Jordan D.
Jellison, Evan R.
Ménoret, Antoine
Ozawa, Manabu
Rodriguez-Oquendo, Annabelle
Vella, Anthony T.
Murphy, Patrick A.
Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
title Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
title_full Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
title_fullStr Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
title_full_unstemmed Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
title_short Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
title_sort splice factor polypyrimidine tract-binding protein 1 (ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335344/
https://www.ncbi.nlm.nih.gov/pubmed/35858420
http://dx.doi.org/10.1073/pnas.2122227119
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