Cargando…

Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy

OBJECTIVE: This study aimed to investigate the effect of Zinc Finger E-box Binding Homeobox 1 (ZEB1) regulation by Micro Ribonucleic acid (miR)-448 on Breast Cancer (BC) cells and their sensitivity to chemotherapy. METHODS: miR-448 and ZEB1 mRNA levels in BC and normal tissues were detected by qPCR,...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Yizhou, Sun, Li, Zhang, Yangmei, Chen, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335378/
https://www.ncbi.nlm.nih.gov/pubmed/35905576
http://dx.doi.org/10.1016/j.clinsp.2022.100089
_version_ 1784759325813637120
author Zhou, Yizhou
Sun, Li
Zhang, Yangmei
Chen, Kai
author_facet Zhou, Yizhou
Sun, Li
Zhang, Yangmei
Chen, Kai
author_sort Zhou, Yizhou
collection PubMed
description OBJECTIVE: This study aimed to investigate the effect of Zinc Finger E-box Binding Homeobox 1 (ZEB1) regulation by Micro Ribonucleic acid (miR)-448 on Breast Cancer (BC) cells and their sensitivity to chemotherapy. METHODS: miR-448 and ZEB1 mRNA levels in BC and normal tissues were detected by qPCR, and ZEB1 protein was detected by Western Blotting (WB). The correlation between miR-448 and tumor metastasis, clinical staging, and ZEB1 expression was analyzed. MCF-7 cells were transfected or co-transfected with the miR-448 mimic, oe-ZEB1, or their negative controls. Changes in miR-448 and ZEB1 expression were detected by qPCR and WB. Cell proliferation was determined by CCK-8 assays, invasion changes were analyzed by Transwell assays, and apoptosis was detected by flow cytometry. RESULTS: miR-448 expression in BC tissues was lower than that in normal tissues, while ZEB1 expression was increased in the former. ZEB1 expression was lower in BC patients with lymph node metastasis than in those without. In patients with clinical stage I–III BC, miR-448 expression decreased with an increase in tumor stage, which was negatively correlated with ZEB1 expression. Upregulation of miR-448 expression can suppress MCF-7 cell proliferation and invasion and promote apoptosis. Upregulation of ZEB1 expression in cells overexpressing miR-448 can partially reverse the inhibition of BC cell growth induced by miR-448. miR-448 can enhance the sensitivity of cells toward paclitaxel and 5-fluorouracil. CONCLUSIONS: miR-448 suppresses cell proliferation and invasion and promotes apoptosis by targeting ZEB1. Moreover, it can increase the sensitivity of cells toward paclitaxel and 5-fluorouracil.
format Online
Article
Text
id pubmed-9335378
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo
record_format MEDLINE/PubMed
spelling pubmed-93353782022-07-30 Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy Zhou, Yizhou Sun, Li Zhang, Yangmei Chen, Kai Clinics (Sao Paulo) Original Articles OBJECTIVE: This study aimed to investigate the effect of Zinc Finger E-box Binding Homeobox 1 (ZEB1) regulation by Micro Ribonucleic acid (miR)-448 on Breast Cancer (BC) cells and their sensitivity to chemotherapy. METHODS: miR-448 and ZEB1 mRNA levels in BC and normal tissues were detected by qPCR, and ZEB1 protein was detected by Western Blotting (WB). The correlation between miR-448 and tumor metastasis, clinical staging, and ZEB1 expression was analyzed. MCF-7 cells were transfected or co-transfected with the miR-448 mimic, oe-ZEB1, or their negative controls. Changes in miR-448 and ZEB1 expression were detected by qPCR and WB. Cell proliferation was determined by CCK-8 assays, invasion changes were analyzed by Transwell assays, and apoptosis was detected by flow cytometry. RESULTS: miR-448 expression in BC tissues was lower than that in normal tissues, while ZEB1 expression was increased in the former. ZEB1 expression was lower in BC patients with lymph node metastasis than in those without. In patients with clinical stage I–III BC, miR-448 expression decreased with an increase in tumor stage, which was negatively correlated with ZEB1 expression. Upregulation of miR-448 expression can suppress MCF-7 cell proliferation and invasion and promote apoptosis. Upregulation of ZEB1 expression in cells overexpressing miR-448 can partially reverse the inhibition of BC cell growth induced by miR-448. miR-448 can enhance the sensitivity of cells toward paclitaxel and 5-fluorouracil. CONCLUSIONS: miR-448 suppresses cell proliferation and invasion and promotes apoptosis by targeting ZEB1. Moreover, it can increase the sensitivity of cells toward paclitaxel and 5-fluorouracil. Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2022-07-26 /pmc/articles/PMC9335378/ /pubmed/35905576 http://dx.doi.org/10.1016/j.clinsp.2022.100089 Text en © 2022 Published by Elsevier España, S.L.U. on behalf of HCFMUSP. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Articles
Zhou, Yizhou
Sun, Li
Zhang, Yangmei
Chen, Kai
Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
title Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
title_full Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
title_fullStr Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
title_full_unstemmed Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
title_short Micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
title_sort micro ribonucleic acid-448 regulates zinc finger e-box binding homeobox 1 to inhibit the growth of breast cancer cells and increase their sensitivity to chemotherapy
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335378/
https://www.ncbi.nlm.nih.gov/pubmed/35905576
http://dx.doi.org/10.1016/j.clinsp.2022.100089
work_keys_str_mv AT zhouyizhou microribonucleicacid448regulateszincfingereboxbindinghomeobox1toinhibitthegrowthofbreastcancercellsandincreasetheirsensitivitytochemotherapy
AT sunli microribonucleicacid448regulateszincfingereboxbindinghomeobox1toinhibitthegrowthofbreastcancercellsandincreasetheirsensitivitytochemotherapy
AT zhangyangmei microribonucleicacid448regulateszincfingereboxbindinghomeobox1toinhibitthegrowthofbreastcancercellsandincreasetheirsensitivitytochemotherapy
AT chenkai microribonucleicacid448regulateszincfingereboxbindinghomeobox1toinhibitthegrowthofbreastcancercellsandincreasetheirsensitivitytochemotherapy