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Characterization of Nanoparticles in Diverse Mixtures Using Localized Surface Plasmon Resonance and Nanoparticle Tracking by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics
[Image: see text] Redox magnetohydrodynamics (RMHD) microfluidics is coupled with dark-field microscopy (DFM) to offer high-throughput single-nanoparticle (NP) differentiation in situ and operando in a flowing mixture by localized surface plasmon resonance (LSPR) and tracking of NPs. The color of th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335947/ https://www.ncbi.nlm.nih.gov/pubmed/35915589 http://dx.doi.org/10.1021/acsphyschemau.1c00046 |
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author | Sikes, Jazlynn C. Wonner, Kevin Nicholson, Aaron Cignoni, Paolo Fritsch, Ingrid Tschulik, Kristina |
author_facet | Sikes, Jazlynn C. Wonner, Kevin Nicholson, Aaron Cignoni, Paolo Fritsch, Ingrid Tschulik, Kristina |
author_sort | Sikes, Jazlynn C. |
collection | PubMed |
description | [Image: see text] Redox magnetohydrodynamics (RMHD) microfluidics is coupled with dark-field microscopy (DFM) to offer high-throughput single-nanoparticle (NP) differentiation in situ and operando in a flowing mixture by localized surface plasmon resonance (LSPR) and tracking of NPs. The color of the scattered light allows visualization of the NPs below the diffraction limit. Their Brownian motion in 1-D superimposed on and perpendicular to the RMHD trajectory yields their diffusion coefficients. LSPR and diffusion coefficients provide two orthogonal modalities for characterization where each depends on a particle’s material composition, shape, size, and interactions with the surrounding medium. RMHD coupled with DFM was demonstrated on a mixture of 82 ± 9 nm silver and 140 ± 10 nm gold-coated silica nanospheres. The two populations of NPs in the mixture were identified by blue/green and orange/red LSPR and their scattering intensity, respectively, and their sizes were further evaluated based on their diffusion coefficients. RMHD microfluidics facilitates high-throughput analysis by moving the sample solution across the wide field of view absent of physical vibrations within the experimental cell. The well-controlled pumping allows for a continuous, reversible, and uniform flow for precise and simultaneous NP tracking of the Brownian motion. Additionally, the amounts of nanomaterials required for the analysis are minimized due to the elimination of an inlet and outlet. Several hundred individual NPs were differentiated from each other in the mixture flowing in forward and reverse directions. The ability to immediately reverse the flow direction also facilitates re-analysis of the NPs, enabling more precise sizing. |
format | Online Article Text |
id | pubmed-9335947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93359472022-07-30 Characterization of Nanoparticles in Diverse Mixtures Using Localized Surface Plasmon Resonance and Nanoparticle Tracking by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics Sikes, Jazlynn C. Wonner, Kevin Nicholson, Aaron Cignoni, Paolo Fritsch, Ingrid Tschulik, Kristina ACS Phys Chem Au [Image: see text] Redox magnetohydrodynamics (RMHD) microfluidics is coupled with dark-field microscopy (DFM) to offer high-throughput single-nanoparticle (NP) differentiation in situ and operando in a flowing mixture by localized surface plasmon resonance (LSPR) and tracking of NPs. The color of the scattered light allows visualization of the NPs below the diffraction limit. Their Brownian motion in 1-D superimposed on and perpendicular to the RMHD trajectory yields their diffusion coefficients. LSPR and diffusion coefficients provide two orthogonal modalities for characterization where each depends on a particle’s material composition, shape, size, and interactions with the surrounding medium. RMHD coupled with DFM was demonstrated on a mixture of 82 ± 9 nm silver and 140 ± 10 nm gold-coated silica nanospheres. The two populations of NPs in the mixture were identified by blue/green and orange/red LSPR and their scattering intensity, respectively, and their sizes were further evaluated based on their diffusion coefficients. RMHD microfluidics facilitates high-throughput analysis by moving the sample solution across the wide field of view absent of physical vibrations within the experimental cell. The well-controlled pumping allows for a continuous, reversible, and uniform flow for precise and simultaneous NP tracking of the Brownian motion. Additionally, the amounts of nanomaterials required for the analysis are minimized due to the elimination of an inlet and outlet. Several hundred individual NPs were differentiated from each other in the mixture flowing in forward and reverse directions. The ability to immediately reverse the flow direction also facilitates re-analysis of the NPs, enabling more precise sizing. American Chemical Society 2022-01-25 /pmc/articles/PMC9335947/ /pubmed/35915589 http://dx.doi.org/10.1021/acsphyschemau.1c00046 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Sikes, Jazlynn C. Wonner, Kevin Nicholson, Aaron Cignoni, Paolo Fritsch, Ingrid Tschulik, Kristina Characterization of Nanoparticles in Diverse Mixtures Using Localized Surface Plasmon Resonance and Nanoparticle Tracking by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics |
title | Characterization of Nanoparticles in Diverse Mixtures
Using Localized Surface Plasmon Resonance and Nanoparticle Tracking
by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics |
title_full | Characterization of Nanoparticles in Diverse Mixtures
Using Localized Surface Plasmon Resonance and Nanoparticle Tracking
by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics |
title_fullStr | Characterization of Nanoparticles in Diverse Mixtures
Using Localized Surface Plasmon Resonance and Nanoparticle Tracking
by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics |
title_full_unstemmed | Characterization of Nanoparticles in Diverse Mixtures
Using Localized Surface Plasmon Resonance and Nanoparticle Tracking
by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics |
title_short | Characterization of Nanoparticles in Diverse Mixtures
Using Localized Surface Plasmon Resonance and Nanoparticle Tracking
by Dark-Field Microscopy with Redox Magnetohydrodynamics Microfluidics |
title_sort | characterization of nanoparticles in diverse mixtures
using localized surface plasmon resonance and nanoparticle tracking
by dark-field microscopy with redox magnetohydrodynamics microfluidics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335947/ https://www.ncbi.nlm.nih.gov/pubmed/35915589 http://dx.doi.org/10.1021/acsphyschemau.1c00046 |
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