Cargando…

Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt

BACKGROUND: Salmonella is one of the most common and economically important zoonotic pathogens. This study aimed to determine the occurrence of Salmonella serovars in sheep and goats and their probable zoonotic risk to humans in Suez Canal area in Egypt. A total of 320 fecal samples from sheep (n = ...

Descripción completa

Detalles Bibliográficos
Autores principales: Hawwas, Hanan Abd El-Halim, Aboueisha, Abdel-Karim Mahmoud, Fadel, Hanaa Mohamed, El-Mahallawy, Heba Sayed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336019/
https://www.ncbi.nlm.nih.gov/pubmed/35906669
http://dx.doi.org/10.1186/s13028-022-00637-y
_version_ 1784759456878297088
author Hawwas, Hanan Abd El-Halim
Aboueisha, Abdel-Karim Mahmoud
Fadel, Hanaa Mohamed
El-Mahallawy, Heba Sayed
author_facet Hawwas, Hanan Abd El-Halim
Aboueisha, Abdel-Karim Mahmoud
Fadel, Hanaa Mohamed
El-Mahallawy, Heba Sayed
author_sort Hawwas, Hanan Abd El-Halim
collection PubMed
description BACKGROUND: Salmonella is one of the most common and economically important zoonotic pathogens. This study aimed to determine the occurrence of Salmonella serovars in sheep and goats and their probable zoonotic risk to humans in Suez Canal area in Egypt. A total of 320 fecal samples from sheep (n = 120), goats (n = 100), and humans (n = 100) were collected and examined for the presence of Salmonella based on cultural and biochemical characteristics, and serological analysis. Moreover, the virulence of the identified Salmonella isolates was assessed by molecular screening for invA, stn, spvC, and sopB virulence genes using PCR. RESULTS: Overall, the occurrence of Salmonella in sheep feces (23.3%) was higher than that in goat feces (7%) and human stool (13%) in the study area. The identified isolates belonged to 12 serotypes; ten, five, and eight from sheep, goats, and humans, respectively. The most frequently identified serotypes were S. Typhimurium from sheep feces, and S. Enteritidis from both goat feces and human stool, with four serotypes; S. Typhimurium, S. Enteritidis, S. Dublin and S. Saintpaul, were mutually shared between all of them. Demographic data revealed that diarrheic sheep (85.7%) and goats (25%) had a higher risk for Salmonella fecal carriage than non-diarrheic ones (19.5% and 6.25%, respectively). The prevalence of Salmonella infection in humans in contact with sheep and goats (28%) was significantly higher than its prevalence in people having a history of contact with animals other than sheep and goats (10%) and those having no history of animal contact (7.3%) (χ(2) = 6.728, P ˂ 0.05). The stn, spvC, and sopB genes were detected in 98.1% of the isolates, with a significant, very strong positive correlation for their mutual presence (P < 0.05). Approximately 40.7% of isolates that carried the invA gene had a non-significant, very weak positive correlation with other virulence genes. The most common genotypic virulence profile for all isolates was stn, spvC, and sopB; however, invA, stn, spvC, and sopB was the frequent virulotype for S. Typhimurium, S. Tsevie, S. Apeyeme, and S. Infantis. CONCLUSIONS: The present study highlights the role of apparently healthy and diarrheic sheep and goats as reservoirs and sources of human infection with virulent Salmonella serovars in the Suez Canal area.
format Online
Article
Text
id pubmed-9336019
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-93360192022-07-30 Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt Hawwas, Hanan Abd El-Halim Aboueisha, Abdel-Karim Mahmoud Fadel, Hanaa Mohamed El-Mahallawy, Heba Sayed Acta Vet Scand Research BACKGROUND: Salmonella is one of the most common and economically important zoonotic pathogens. This study aimed to determine the occurrence of Salmonella serovars in sheep and goats and their probable zoonotic risk to humans in Suez Canal area in Egypt. A total of 320 fecal samples from sheep (n = 120), goats (n = 100), and humans (n = 100) were collected and examined for the presence of Salmonella based on cultural and biochemical characteristics, and serological analysis. Moreover, the virulence of the identified Salmonella isolates was assessed by molecular screening for invA, stn, spvC, and sopB virulence genes using PCR. RESULTS: Overall, the occurrence of Salmonella in sheep feces (23.3%) was higher than that in goat feces (7%) and human stool (13%) in the study area. The identified isolates belonged to 12 serotypes; ten, five, and eight from sheep, goats, and humans, respectively. The most frequently identified serotypes were S. Typhimurium from sheep feces, and S. Enteritidis from both goat feces and human stool, with four serotypes; S. Typhimurium, S. Enteritidis, S. Dublin and S. Saintpaul, were mutually shared between all of them. Demographic data revealed that diarrheic sheep (85.7%) and goats (25%) had a higher risk for Salmonella fecal carriage than non-diarrheic ones (19.5% and 6.25%, respectively). The prevalence of Salmonella infection in humans in contact with sheep and goats (28%) was significantly higher than its prevalence in people having a history of contact with animals other than sheep and goats (10%) and those having no history of animal contact (7.3%) (χ(2) = 6.728, P ˂ 0.05). The stn, spvC, and sopB genes were detected in 98.1% of the isolates, with a significant, very strong positive correlation for their mutual presence (P < 0.05). Approximately 40.7% of isolates that carried the invA gene had a non-significant, very weak positive correlation with other virulence genes. The most common genotypic virulence profile for all isolates was stn, spvC, and sopB; however, invA, stn, spvC, and sopB was the frequent virulotype for S. Typhimurium, S. Tsevie, S. Apeyeme, and S. Infantis. CONCLUSIONS: The present study highlights the role of apparently healthy and diarrheic sheep and goats as reservoirs and sources of human infection with virulent Salmonella serovars in the Suez Canal area. BioMed Central 2022-07-29 /pmc/articles/PMC9336019/ /pubmed/35906669 http://dx.doi.org/10.1186/s13028-022-00637-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Hawwas, Hanan Abd El-Halim
Aboueisha, Abdel-Karim Mahmoud
Fadel, Hanaa Mohamed
El-Mahallawy, Heba Sayed
Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt
title Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt
title_full Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt
title_fullStr Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt
title_full_unstemmed Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt
title_short Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt
title_sort salmonella serovars in sheep and goats and their probable zoonotic potential to humans in suez canal area, egypt
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336019/
https://www.ncbi.nlm.nih.gov/pubmed/35906669
http://dx.doi.org/10.1186/s13028-022-00637-y
work_keys_str_mv AT hawwashananabdelhalim salmonellaserovarsinsheepandgoatsandtheirprobablezoonoticpotentialtohumansinsuezcanalareaegypt
AT aboueishaabdelkarimmahmoud salmonellaserovarsinsheepandgoatsandtheirprobablezoonoticpotentialtohumansinsuezcanalareaegypt
AT fadelhanaamohamed salmonellaserovarsinsheepandgoatsandtheirprobablezoonoticpotentialtohumansinsuezcanalareaegypt
AT elmahallawyhebasayed salmonellaserovarsinsheepandgoatsandtheirprobablezoonoticpotentialtohumansinsuezcanalareaegypt