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Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts

Pistacia lentiscus (Anacardiaceae) is commonly used in folk medicine to treat various diseases. The aim of the present study was to evaluate the hepatoprotective and antioxidant activities of extracts of P. lentiscus leaves (PL) and fruits (PF) against experimentally induced liver damage. Furthermor...

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Autores principales: Mehenni, Chafiaâ, Atmani-Kilani, Dina, Dumarçay, Stéphane, Perrin, Dominique, Gérardin, Philippe, Atmani, Djebbar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336671/
https://www.ncbi.nlm.nih.gov/pubmed/28911573
http://dx.doi.org/10.1016/j.jfda.2016.03.002
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author Mehenni, Chafiaâ
Atmani-Kilani, Dina
Dumarçay, Stéphane
Perrin, Dominique
Gérardin, Philippe
Atmani, Djebbar
author_facet Mehenni, Chafiaâ
Atmani-Kilani, Dina
Dumarçay, Stéphane
Perrin, Dominique
Gérardin, Philippe
Atmani, Djebbar
author_sort Mehenni, Chafiaâ
collection PubMed
description Pistacia lentiscus (Anacardiaceae) is commonly used in folk medicine to treat various diseases. The aim of the present study was to evaluate the hepatoprotective and antioxidant activities of extracts of P. lentiscus leaves (PL) and fruits (PF) against experimentally induced liver damage. Furthermore, characterization of extracts was attempted by a spectroscopic methodology (Fourier transform infrared spectroscopy) and high-performance liquid chromatography with diode array detection analysis. A hepatoprotective potential against paracetamol [165 mg/kg body weight (b.w.)] toxicity was noticed in mice pretreated with the same dose of PL or PF extract (125 mg/kg b.w.) or a combination of both (PL/PF 63/63 mg/kg b.w.), as revealed by an analysis of biochemical parameters (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities and total bilirubin). These results were confirmed by histological examination of the liver, which revealed significant protection against paracetamol-induced hepatic necrosis. Furthermore, PF extract exhibited a promising antidiabetic activity in streptozotocin-induced diabetic rats, similar to the reference drug glibenclamide (0.91 g/L), a result confirmed by in vitro inhibition of α-amylase. We demonstrated that the leaf crude extract showed the best effect in all tested methods, compared to its fruit counterpart, probably due to the presence of higher amounts of phenolic compounds, as determined by phytochemical and Fourier transform infrared spectroscopy analyses. Moreover, high-performance liquid chromatography with diode array detection led to the identification of six compounds for each part of the plant. Gallic acid, a characteristic compound of Pistacia species, was most abundant in leaves and fruits, while luteolin was detected for the first time in fruits. Obtained activities of P. lentiscus extracts may well be due, at least in part, to the presence of the above compounds.
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spelling pubmed-93366712022-08-09 Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts Mehenni, Chafiaâ Atmani-Kilani, Dina Dumarçay, Stéphane Perrin, Dominique Gérardin, Philippe Atmani, Djebbar J Food Drug Anal Original Article Pistacia lentiscus (Anacardiaceae) is commonly used in folk medicine to treat various diseases. The aim of the present study was to evaluate the hepatoprotective and antioxidant activities of extracts of P. lentiscus leaves (PL) and fruits (PF) against experimentally induced liver damage. Furthermore, characterization of extracts was attempted by a spectroscopic methodology (Fourier transform infrared spectroscopy) and high-performance liquid chromatography with diode array detection analysis. A hepatoprotective potential against paracetamol [165 mg/kg body weight (b.w.)] toxicity was noticed in mice pretreated with the same dose of PL or PF extract (125 mg/kg b.w.) or a combination of both (PL/PF 63/63 mg/kg b.w.), as revealed by an analysis of biochemical parameters (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities and total bilirubin). These results were confirmed by histological examination of the liver, which revealed significant protection against paracetamol-induced hepatic necrosis. Furthermore, PF extract exhibited a promising antidiabetic activity in streptozotocin-induced diabetic rats, similar to the reference drug glibenclamide (0.91 g/L), a result confirmed by in vitro inhibition of α-amylase. We demonstrated that the leaf crude extract showed the best effect in all tested methods, compared to its fruit counterpart, probably due to the presence of higher amounts of phenolic compounds, as determined by phytochemical and Fourier transform infrared spectroscopy analyses. Moreover, high-performance liquid chromatography with diode array detection led to the identification of six compounds for each part of the plant. Gallic acid, a characteristic compound of Pistacia species, was most abundant in leaves and fruits, while luteolin was detected for the first time in fruits. Obtained activities of P. lentiscus extracts may well be due, at least in part, to the presence of the above compounds. Taiwan Food and Drug Administration 2016-04-29 /pmc/articles/PMC9336671/ /pubmed/28911573 http://dx.doi.org/10.1016/j.jfda.2016.03.002 Text en © 2016 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Mehenni, Chafiaâ
Atmani-Kilani, Dina
Dumarçay, Stéphane
Perrin, Dominique
Gérardin, Philippe
Atmani, Djebbar
Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts
title Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts
title_full Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts
title_fullStr Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts
title_full_unstemmed Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts
title_short Hepatoprotective and antidiabetic effects of Pistacia lentiscus leaf and fruit extracts
title_sort hepatoprotective and antidiabetic effects of pistacia lentiscus leaf and fruit extracts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336671/
https://www.ncbi.nlm.nih.gov/pubmed/28911573
http://dx.doi.org/10.1016/j.jfda.2016.03.002
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