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Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia

Benign prostatic hyperplasia (BPH) is a commonly occurring disease in aging men. It involves cellular proliferation of stromal and glandular tissues leading to prostate enlargement. Current drug therapies show several adverse effects such as sexual dysfunctions and cardiovascular side effects. There...

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Autores principales: Sachdeva, Ruchi, Kaur, Navneet, Kapoor, Paras, Singla, Pooja, Thakur, Nidhi, Singhmar, Sakshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336786/
https://www.ncbi.nlm.nih.gov/pubmed/36059933
http://dx.doi.org/10.22099/mbrc.2022.43721.1746
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author Sachdeva, Ruchi
Kaur, Navneet
Kapoor, Paras
Singla, Pooja
Thakur, Nidhi
Singhmar, Sakshi
author_facet Sachdeva, Ruchi
Kaur, Navneet
Kapoor, Paras
Singla, Pooja
Thakur, Nidhi
Singhmar, Sakshi
author_sort Sachdeva, Ruchi
collection PubMed
description Benign prostatic hyperplasia (BPH) is a commonly occurring disease in aging men. It involves cellular proliferation of stromal and glandular tissues leading to prostate enlargement. Current drug therapies show several adverse effects such as sexual dysfunctions and cardiovascular side effects. Therefore, there is a need to develop more effective medical treatment for BPH. In this regard, we aimed to identify genes which play a critical role in BPH. We have obtained the dataset of differentially expressed genes (DEGs) of BPH from NCBI GEO. DEGs were investigated in the context of their protein-protein interactions (PPI). Hub genes i.e. genes associated with BPH were scrutinized based on the topological parameters of the PPI network. These were analyzed for functional annotations, pathway enrichment analysis and transcriptional regulation. In total, 38 hub genes were identified. Hub genes such as transcription factor activator protein-1 and adiponectin were found to play key roles in cellular proliferation and inflammation. Another gene peroxisome proliferator activated receptor gamma was suggested to cause obesity, a common comorbidity of BPH. Moreover, our results indicated an important role of transforming growth factor-beta (TGF-β) signaling and smooth muscle cell proliferation which may be responsible for prostate overgrowth and associated lower urinary tract symptoms frequently encountered in BPH patients. Zinc finger protein Snai1 was the most prominent transcription factor regulating the expression of hub genes that participate in TGF-β signaling. Overall, our study has revealed significant hub genes that can be employed as drug targets to develop potential therapeutic interventions to treat BPH.
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spelling pubmed-93367862022-09-03 Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia Sachdeva, Ruchi Kaur, Navneet Kapoor, Paras Singla, Pooja Thakur, Nidhi Singhmar, Sakshi Mol Biol Res Commun Original Article Benign prostatic hyperplasia (BPH) is a commonly occurring disease in aging men. It involves cellular proliferation of stromal and glandular tissues leading to prostate enlargement. Current drug therapies show several adverse effects such as sexual dysfunctions and cardiovascular side effects. Therefore, there is a need to develop more effective medical treatment for BPH. In this regard, we aimed to identify genes which play a critical role in BPH. We have obtained the dataset of differentially expressed genes (DEGs) of BPH from NCBI GEO. DEGs were investigated in the context of their protein-protein interactions (PPI). Hub genes i.e. genes associated with BPH were scrutinized based on the topological parameters of the PPI network. These were analyzed for functional annotations, pathway enrichment analysis and transcriptional regulation. In total, 38 hub genes were identified. Hub genes such as transcription factor activator protein-1 and adiponectin were found to play key roles in cellular proliferation and inflammation. Another gene peroxisome proliferator activated receptor gamma was suggested to cause obesity, a common comorbidity of BPH. Moreover, our results indicated an important role of transforming growth factor-beta (TGF-β) signaling and smooth muscle cell proliferation which may be responsible for prostate overgrowth and associated lower urinary tract symptoms frequently encountered in BPH patients. Zinc finger protein Snai1 was the most prominent transcription factor regulating the expression of hub genes that participate in TGF-β signaling. Overall, our study has revealed significant hub genes that can be employed as drug targets to develop potential therapeutic interventions to treat BPH. Shiraz University 2022-06 /pmc/articles/PMC9336786/ /pubmed/36059933 http://dx.doi.org/10.22099/mbrc.2022.43721.1746 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License. https://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Sachdeva, Ruchi
Kaur, Navneet
Kapoor, Paras
Singla, Pooja
Thakur, Nidhi
Singhmar, Sakshi
Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
title Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
title_full Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
title_fullStr Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
title_full_unstemmed Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
title_short Computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
title_sort computational analysis of protein-protein interaction network of differentially expressed genes in benign prostatic hyperplasia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336786/
https://www.ncbi.nlm.nih.gov/pubmed/36059933
http://dx.doi.org/10.22099/mbrc.2022.43721.1746
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