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Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients

BACKGROUND: Vitiligo is one of the most common hypomelanoses, in which the destruction of functioning melanocytes causes depigmentation of the skin, hair and mucous membranes. The genes encrypting brain-derived neurotrophic factor (BDNF) and corticotropin releasing hormone (CRH) might be the conceiv...

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Autores principales: Kussainova, Assiya, Kassym, Laura, Bekenova, Nazira, Akhmetova, Almira, Glushkova, Natalya, Kussainov, Almas, Urazalina, Zhanar, Yurkovskaya, Oxana, Smail, Yerbol, Pak, Laura, Semenova, Yuliya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337645/
https://www.ncbi.nlm.nih.gov/pubmed/35905107
http://dx.doi.org/10.1371/journal.pone.0271719
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author Kussainova, Assiya
Kassym, Laura
Bekenova, Nazira
Akhmetova, Almira
Glushkova, Natalya
Kussainov, Almas
Urazalina, Zhanar
Yurkovskaya, Oxana
Smail, Yerbol
Pak, Laura
Semenova, Yuliya
author_facet Kussainova, Assiya
Kassym, Laura
Bekenova, Nazira
Akhmetova, Almira
Glushkova, Natalya
Kussainov, Almas
Urazalina, Zhanar
Yurkovskaya, Oxana
Smail, Yerbol
Pak, Laura
Semenova, Yuliya
author_sort Kussainova, Assiya
collection PubMed
description BACKGROUND: Vitiligo is one of the most common hypomelanoses, in which the destruction of functioning melanocytes causes depigmentation of the skin, hair and mucous membranes. The genes encrypting brain-derived neurotrophic factor (BDNF) and corticotropin releasing hormone (CRH) might be the conceivable contributors to the development of vitiligo. This study was aimed at investigation of the serum levels of BDNF and CRH as well as their selected single nucleotide polymorphisms (SNPs) in vitiligo patients in comparison with the healthy controls. METHODS: The cross-sectional study was carried out between October 2020 and June 2021 in 93 vitiligo patients (age range from 23 to 48 years) and 132 healthy controls (age range from 24 to 52 years). The psychological status of study participants was evaluated using the Generalized Anxiety Disorder-7 (GAD-7) scale. Serum levels of BDNF and CRH were measured with the help of a commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kit. Genotyping for the rs11030094 polymorphism of the BDNF gene and for the rs242924 polymorphism of the corticotropin releasing hormone receptor 1 (CRH-R1) gene was performed by a real-time polymerase chain reaction (PCR). RESULTS: There was a significant relationship between the CRH-R1 rs242924 and BDNF rs11030094 polymorphisms and vitiligo. Moreover, serum levels of neurotransmitters differed significantly between vitiligo and control groups and were associated with the CRH-R1 rs242924 and BDNF rs11030094 SNPs. CONCLUSIONS: Our findings demonstrated the association between CRH-R1 rs242924 and BDNF rs11030094 polymorphisms and vitiligo. Further studies need to be carried out in vitiligo patients to confirm the results observed.
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spelling pubmed-93376452022-07-30 Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients Kussainova, Assiya Kassym, Laura Bekenova, Nazira Akhmetova, Almira Glushkova, Natalya Kussainov, Almas Urazalina, Zhanar Yurkovskaya, Oxana Smail, Yerbol Pak, Laura Semenova, Yuliya PLoS One Research Article BACKGROUND: Vitiligo is one of the most common hypomelanoses, in which the destruction of functioning melanocytes causes depigmentation of the skin, hair and mucous membranes. The genes encrypting brain-derived neurotrophic factor (BDNF) and corticotropin releasing hormone (CRH) might be the conceivable contributors to the development of vitiligo. This study was aimed at investigation of the serum levels of BDNF and CRH as well as their selected single nucleotide polymorphisms (SNPs) in vitiligo patients in comparison with the healthy controls. METHODS: The cross-sectional study was carried out between October 2020 and June 2021 in 93 vitiligo patients (age range from 23 to 48 years) and 132 healthy controls (age range from 24 to 52 years). The psychological status of study participants was evaluated using the Generalized Anxiety Disorder-7 (GAD-7) scale. Serum levels of BDNF and CRH were measured with the help of a commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kit. Genotyping for the rs11030094 polymorphism of the BDNF gene and for the rs242924 polymorphism of the corticotropin releasing hormone receptor 1 (CRH-R1) gene was performed by a real-time polymerase chain reaction (PCR). RESULTS: There was a significant relationship between the CRH-R1 rs242924 and BDNF rs11030094 polymorphisms and vitiligo. Moreover, serum levels of neurotransmitters differed significantly between vitiligo and control groups and were associated with the CRH-R1 rs242924 and BDNF rs11030094 SNPs. CONCLUSIONS: Our findings demonstrated the association between CRH-R1 rs242924 and BDNF rs11030094 polymorphisms and vitiligo. Further studies need to be carried out in vitiligo patients to confirm the results observed. Public Library of Science 2022-07-29 /pmc/articles/PMC9337645/ /pubmed/35905107 http://dx.doi.org/10.1371/journal.pone.0271719 Text en © 2022 Kussainova et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kussainova, Assiya
Kassym, Laura
Bekenova, Nazira
Akhmetova, Almira
Glushkova, Natalya
Kussainov, Almas
Urazalina, Zhanar
Yurkovskaya, Oxana
Smail, Yerbol
Pak, Laura
Semenova, Yuliya
Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients
title Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients
title_full Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients
title_fullStr Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients
title_full_unstemmed Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients
title_short Gene polymorphisms and serum levels of BDNF and CRH in vitiligo patients
title_sort gene polymorphisms and serum levels of bdnf and crh in vitiligo patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337645/
https://www.ncbi.nlm.nih.gov/pubmed/35905107
http://dx.doi.org/10.1371/journal.pone.0271719
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