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Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America

The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance of the other HR-HPV differs slightly by geographical region. Here, we studied the HPV genotype distribution of HPV positive Latin American (LA) women by histological g...

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Autores principales: Correa, Rita Mariel, Baena, Armando, Valls, Joan, Colucci, María Celeste, Mendoza, Laura, Rol, Maryluz, Wiesner, Carolina, Ferrera, Annabelle, Fellner, María Dolores, González, Joaquín Víctor, Basiletti, Jorge Alejandro, Mongelos, Pamela, Rodriguez de la Peña, Mercedes, Saino, Agustina, Kasamatsu, Elena, Velarde, Carlos, Macavilca, Ninoska, Martinez, Sandra, Venegas, Gino, Calderón, Alejandro, Rodriguez, Guillermo, Barrios, Hernán, Herrero, Rolando, Almonte, Maribel, Picconi, María Alejandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337688/
https://www.ncbi.nlm.nih.gov/pubmed/35905130
http://dx.doi.org/10.1371/journal.pone.0272205
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author Correa, Rita Mariel
Baena, Armando
Valls, Joan
Colucci, María Celeste
Mendoza, Laura
Rol, Maryluz
Wiesner, Carolina
Ferrera, Annabelle
Fellner, María Dolores
González, Joaquín Víctor
Basiletti, Jorge Alejandro
Mongelos, Pamela
Rodriguez de la Peña, Mercedes
Saino, Agustina
Kasamatsu, Elena
Velarde, Carlos
Macavilca, Ninoska
Martinez, Sandra
Venegas, Gino
Calderón, Alejandro
Rodriguez, Guillermo
Barrios, Hernán
Herrero, Rolando
Almonte, Maribel
Picconi, María Alejandra
author_facet Correa, Rita Mariel
Baena, Armando
Valls, Joan
Colucci, María Celeste
Mendoza, Laura
Rol, Maryluz
Wiesner, Carolina
Ferrera, Annabelle
Fellner, María Dolores
González, Joaquín Víctor
Basiletti, Jorge Alejandro
Mongelos, Pamela
Rodriguez de la Peña, Mercedes
Saino, Agustina
Kasamatsu, Elena
Velarde, Carlos
Macavilca, Ninoska
Martinez, Sandra
Venegas, Gino
Calderón, Alejandro
Rodriguez, Guillermo
Barrios, Hernán
Herrero, Rolando
Almonte, Maribel
Picconi, María Alejandra
author_sort Correa, Rita Mariel
collection PubMed
description The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance of the other HR-HPV differs slightly by geographical region. Here, we studied the HPV genotype distribution of HPV positive Latin American (LA) women by histological grade, in a sub-cohort from the ESTAMPA study; we also explored the association of age-specific HPV genotypes in severe lesions. Cervical samples from 1,252 participants (854 ≤CIN1, 121 CIN2, 194 CIN3 and 83 CC) were genotyped by two PCRs-Reverse Blotting Hybridization strategies: i) Broad-Spectrum General Primers 5+/6+ and ii) PGMY9/11 PCRs. HPV16 was the most frequently found genotype in all histological grades, and increased with the severity of lesions from 14.5% in ≤ CIN1, 19.8% in CIN2, 51.5% in CIN3 to 65.1% in CC (p < 0.001). For the remaining HR-HPVs their frequency in CC did not increase when compared to less severe categories. The nonavalent vaccine HR-types ranked at the top in CC, the dominant ones being HPV16 and HPV45. HR-HPV single infection occurs, respectively, in 57.1% and 57.0% of ≤CIN1 and CIN2, increasing to 72.2% and 91.6% in CIN3 and CC (p<0.001). No association between age and HPV type was observed in CC, although the risk of HPV16 infection in CIN3 cases increased with age. Results confirm the relevance of HPV16 in the whole clinical spectrum, with a strong rise of its proportion in CIN3 and cancer. This information will be relevant in evaluating the impact of HPV vaccination, as a baseline against which to compare genotype changes in HPV type-specific distribution as vaccinated women participate in screening in LA region. Likewise, these data may help select the best HPV testing system for HPV-based efficient, affordable, and sustainable screening programmes.
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spelling pubmed-93376882022-07-30 Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America Correa, Rita Mariel Baena, Armando Valls, Joan Colucci, María Celeste Mendoza, Laura Rol, Maryluz Wiesner, Carolina Ferrera, Annabelle Fellner, María Dolores González, Joaquín Víctor Basiletti, Jorge Alejandro Mongelos, Pamela Rodriguez de la Peña, Mercedes Saino, Agustina Kasamatsu, Elena Velarde, Carlos Macavilca, Ninoska Martinez, Sandra Venegas, Gino Calderón, Alejandro Rodriguez, Guillermo Barrios, Hernán Herrero, Rolando Almonte, Maribel Picconi, María Alejandra PLoS One Research Article The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance of the other HR-HPV differs slightly by geographical region. Here, we studied the HPV genotype distribution of HPV positive Latin American (LA) women by histological grade, in a sub-cohort from the ESTAMPA study; we also explored the association of age-specific HPV genotypes in severe lesions. Cervical samples from 1,252 participants (854 ≤CIN1, 121 CIN2, 194 CIN3 and 83 CC) were genotyped by two PCRs-Reverse Blotting Hybridization strategies: i) Broad-Spectrum General Primers 5+/6+ and ii) PGMY9/11 PCRs. HPV16 was the most frequently found genotype in all histological grades, and increased with the severity of lesions from 14.5% in ≤ CIN1, 19.8% in CIN2, 51.5% in CIN3 to 65.1% in CC (p < 0.001). For the remaining HR-HPVs their frequency in CC did not increase when compared to less severe categories. The nonavalent vaccine HR-types ranked at the top in CC, the dominant ones being HPV16 and HPV45. HR-HPV single infection occurs, respectively, in 57.1% and 57.0% of ≤CIN1 and CIN2, increasing to 72.2% and 91.6% in CIN3 and CC (p<0.001). No association between age and HPV type was observed in CC, although the risk of HPV16 infection in CIN3 cases increased with age. Results confirm the relevance of HPV16 in the whole clinical spectrum, with a strong rise of its proportion in CIN3 and cancer. This information will be relevant in evaluating the impact of HPV vaccination, as a baseline against which to compare genotype changes in HPV type-specific distribution as vaccinated women participate in screening in LA region. Likewise, these data may help select the best HPV testing system for HPV-based efficient, affordable, and sustainable screening programmes. Public Library of Science 2022-07-29 /pmc/articles/PMC9337688/ /pubmed/35905130 http://dx.doi.org/10.1371/journal.pone.0272205 Text en © 2022 Correa et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Correa, Rita Mariel
Baena, Armando
Valls, Joan
Colucci, María Celeste
Mendoza, Laura
Rol, Maryluz
Wiesner, Carolina
Ferrera, Annabelle
Fellner, María Dolores
González, Joaquín Víctor
Basiletti, Jorge Alejandro
Mongelos, Pamela
Rodriguez de la Peña, Mercedes
Saino, Agustina
Kasamatsu, Elena
Velarde, Carlos
Macavilca, Ninoska
Martinez, Sandra
Venegas, Gino
Calderón, Alejandro
Rodriguez, Guillermo
Barrios, Hernán
Herrero, Rolando
Almonte, Maribel
Picconi, María Alejandra
Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America
title Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America
title_full Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America
title_fullStr Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America
title_full_unstemmed Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America
title_short Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America
title_sort distribution of human papillomavirus genotypes by severity of cervical lesions in hpv screened positive women from the estampa study in latin america
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337688/
https://www.ncbi.nlm.nih.gov/pubmed/35905130
http://dx.doi.org/10.1371/journal.pone.0272205
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