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A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source

Many in vivo biological techniques, such as fluorescence imaging, photodynamic therapy, and optogenetics, require light delivery into biological tissues. The limited tissue penetration of visible light discourages the use of external light sources and calls for the development of light sources that...

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Autores principales: Yang, Fan, Wu, Xiang, Cui, Han, Ou, Zihao, Jiang, Shan, Cai, Sa, Zhou, Qi, Wong, Bryce G., Huang, Hans, Hong, Guosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337768/
https://www.ncbi.nlm.nih.gov/pubmed/35905189
http://dx.doi.org/10.1126/sciadv.abo6743
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author Yang, Fan
Wu, Xiang
Cui, Han
Ou, Zihao
Jiang, Shan
Cai, Sa
Zhou, Qi
Wong, Bryce G.
Huang, Hans
Hong, Guosong
author_facet Yang, Fan
Wu, Xiang
Cui, Han
Ou, Zihao
Jiang, Shan
Cai, Sa
Zhou, Qi
Wong, Bryce G.
Huang, Hans
Hong, Guosong
author_sort Yang, Fan
collection PubMed
description Many in vivo biological techniques, such as fluorescence imaging, photodynamic therapy, and optogenetics, require light delivery into biological tissues. The limited tissue penetration of visible light discourages the use of external light sources and calls for the development of light sources that can be delivered in vivo. A promising material for internal light delivery is persistent phosphors; however, there is a scarcity of materials with strong persistent luminescence of visible light in a stable colloid to facilitate systemic delivery in vivo. Here, we used a bioinspired demineralization (BID) strategy to synthesize stable colloidal solutions of solid-state phosphors in the range of 470 to 650 nm and diameters down to 20 nm. The exceptional brightness of BID-produced colloids enables their utility as multicolor luminescent tags in vivo with favorable biocompatibility. Because of their stable dispersion in water, BID-produced nanophosphors can be delivered systemically, acting as an intravascular colloidal light source to internally excite genetically encoded fluorescent reporters within the mouse brain.
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spelling pubmed-93377682022-08-09 A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source Yang, Fan Wu, Xiang Cui, Han Ou, Zihao Jiang, Shan Cai, Sa Zhou, Qi Wong, Bryce G. Huang, Hans Hong, Guosong Sci Adv Physical and Materials Sciences Many in vivo biological techniques, such as fluorescence imaging, photodynamic therapy, and optogenetics, require light delivery into biological tissues. The limited tissue penetration of visible light discourages the use of external light sources and calls for the development of light sources that can be delivered in vivo. A promising material for internal light delivery is persistent phosphors; however, there is a scarcity of materials with strong persistent luminescence of visible light in a stable colloid to facilitate systemic delivery in vivo. Here, we used a bioinspired demineralization (BID) strategy to synthesize stable colloidal solutions of solid-state phosphors in the range of 470 to 650 nm and diameters down to 20 nm. The exceptional brightness of BID-produced colloids enables their utility as multicolor luminescent tags in vivo with favorable biocompatibility. Because of their stable dispersion in water, BID-produced nanophosphors can be delivered systemically, acting as an intravascular colloidal light source to internally excite genetically encoded fluorescent reporters within the mouse brain. American Association for the Advancement of Science 2022-07-29 /pmc/articles/PMC9337768/ /pubmed/35905189 http://dx.doi.org/10.1126/sciadv.abo6743 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Yang, Fan
Wu, Xiang
Cui, Han
Ou, Zihao
Jiang, Shan
Cai, Sa
Zhou, Qi
Wong, Bryce G.
Huang, Hans
Hong, Guosong
A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
title A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
title_full A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
title_fullStr A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
title_full_unstemmed A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
title_short A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
title_sort biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337768/
https://www.ncbi.nlm.nih.gov/pubmed/35905189
http://dx.doi.org/10.1126/sciadv.abo6743
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