KOPI: Kinase inhibitOr Proteome Impact analysis

Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s uti...

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Detalles Bibliográficos
Autores principales: Li, Ginny Xiaohe, Zhao, Tianyun, Wang, Loo Chien, Choi, Hyungwon, Lim, Yan Ting, Sobota, Radoslaw M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338059/
https://www.ncbi.nlm.nih.gov/pubmed/35906361
http://dx.doi.org/10.1038/s41598-022-16557-w
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author Li, Ginny Xiaohe
Zhao, Tianyun
Wang, Loo Chien
Choi, Hyungwon
Lim, Yan Ting
Sobota, Radoslaw M.
author_facet Li, Ginny Xiaohe
Zhao, Tianyun
Wang, Loo Chien
Choi, Hyungwon
Lim, Yan Ting
Sobota, Radoslaw M.
author_sort Li, Ginny Xiaohe
collection PubMed
description Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine’s non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation.
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spelling pubmed-93380592022-07-31 KOPI: Kinase inhibitOr Proteome Impact analysis Li, Ginny Xiaohe Zhao, Tianyun Wang, Loo Chien Choi, Hyungwon Lim, Yan Ting Sobota, Radoslaw M. Sci Rep Article Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine’s non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation. Nature Publishing Group UK 2022-07-29 /pmc/articles/PMC9338059/ /pubmed/35906361 http://dx.doi.org/10.1038/s41598-022-16557-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Ginny Xiaohe
Zhao, Tianyun
Wang, Loo Chien
Choi, Hyungwon
Lim, Yan Ting
Sobota, Radoslaw M.
KOPI: Kinase inhibitOr Proteome Impact analysis
title KOPI: Kinase inhibitOr Proteome Impact analysis
title_full KOPI: Kinase inhibitOr Proteome Impact analysis
title_fullStr KOPI: Kinase inhibitOr Proteome Impact analysis
title_full_unstemmed KOPI: Kinase inhibitOr Proteome Impact analysis
title_short KOPI: Kinase inhibitOr Proteome Impact analysis
title_sort kopi: kinase inhibitor proteome impact analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338059/
https://www.ncbi.nlm.nih.gov/pubmed/35906361
http://dx.doi.org/10.1038/s41598-022-16557-w
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