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The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant
The scarce research about the worldwide used glyphosate-based herbicide Roundup is controversial in human reproduction, especially spermatozoa. This study investigates the in vitro effect in human spermatozoa of Roundup Ultra Plus (RUP), its active ingredient glyphosate and its non-active, surfactan...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338072/ https://www.ncbi.nlm.nih.gov/pubmed/35906274 http://dx.doi.org/10.1038/s41598-022-17023-3 |
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author | Torres-Badia, Mercedes Solar-Malaga, Soraya Serrano, Rebeca Garcia-Marin, Luis J. Bragado, M. Julia |
author_facet | Torres-Badia, Mercedes Solar-Malaga, Soraya Serrano, Rebeca Garcia-Marin, Luis J. Bragado, M. Julia |
author_sort | Torres-Badia, Mercedes |
collection | PubMed |
description | The scarce research about the worldwide used glyphosate-based herbicide Roundup is controversial in human reproduction, especially spermatozoa. This study investigates the in vitro effect in human spermatozoa of Roundup Ultra Plus (RUP), its active ingredient glyphosate and its non-active, surfactant. Human spermatozoa were incubated (1 h, 37 °C) in presence/absence of RUP 0.01%, glyphosate, or equivalent surfactant concentration. Motility and sperm parameters were analyzed by C.A.S.A and flow cytometry, respectively. RUP significantly increases sperm plasma membrane lipid disorganization in a concentration-dependent manner while it decreases plasma membrane integrity. RUP significantly increases the death spermatozoa population after A23187-induced acrosome reaction, without affecting sperm viability, mitochondrial membrane potential, ROS content, acrosome membrane damage, phosphatidylserine exposure, A23187-induced acrosome reaction or GSK3 phosphorylation. RUP also significantly decreases motile and the a + b sperm populations. Interestingly, all sperm effects caused by RUP 0.01% are mimicked by its surfactant POEA at equivalent concentration. However, glyphosate does not affect any sperm parameter, even using 10-times higher concentration than the RUP 0.01% equivalent. RUP disturbs lipid organization and integrity of human sperm plasma membrane and reduces motility, without affecting viability or functional parameters. Importantly, RUP adverse effects in human spermatozoa are caused by the surfactant and no by glyphosate. |
format | Online Article Text |
id | pubmed-9338072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93380722022-07-31 The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant Torres-Badia, Mercedes Solar-Malaga, Soraya Serrano, Rebeca Garcia-Marin, Luis J. Bragado, M. Julia Sci Rep Article The scarce research about the worldwide used glyphosate-based herbicide Roundup is controversial in human reproduction, especially spermatozoa. This study investigates the in vitro effect in human spermatozoa of Roundup Ultra Plus (RUP), its active ingredient glyphosate and its non-active, surfactant. Human spermatozoa were incubated (1 h, 37 °C) in presence/absence of RUP 0.01%, glyphosate, or equivalent surfactant concentration. Motility and sperm parameters were analyzed by C.A.S.A and flow cytometry, respectively. RUP significantly increases sperm plasma membrane lipid disorganization in a concentration-dependent manner while it decreases plasma membrane integrity. RUP significantly increases the death spermatozoa population after A23187-induced acrosome reaction, without affecting sperm viability, mitochondrial membrane potential, ROS content, acrosome membrane damage, phosphatidylserine exposure, A23187-induced acrosome reaction or GSK3 phosphorylation. RUP also significantly decreases motile and the a + b sperm populations. Interestingly, all sperm effects caused by RUP 0.01% are mimicked by its surfactant POEA at equivalent concentration. However, glyphosate does not affect any sperm parameter, even using 10-times higher concentration than the RUP 0.01% equivalent. RUP disturbs lipid organization and integrity of human sperm plasma membrane and reduces motility, without affecting viability or functional parameters. Importantly, RUP adverse effects in human spermatozoa are caused by the surfactant and no by glyphosate. Nature Publishing Group UK 2022-07-29 /pmc/articles/PMC9338072/ /pubmed/35906274 http://dx.doi.org/10.1038/s41598-022-17023-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Torres-Badia, Mercedes Solar-Malaga, Soraya Serrano, Rebeca Garcia-Marin, Luis J. Bragado, M. Julia The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant |
title | The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant |
title_full | The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant |
title_fullStr | The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant |
title_full_unstemmed | The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant |
title_short | The adverse impact of herbicide Roundup Ultra Plus in human spermatozoa plasma membrane is caused by its surfactant |
title_sort | adverse impact of herbicide roundup ultra plus in human spermatozoa plasma membrane is caused by its surfactant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338072/ https://www.ncbi.nlm.nih.gov/pubmed/35906274 http://dx.doi.org/10.1038/s41598-022-17023-3 |
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