Cargando…
Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method
Microbiomes are essential to a host’s physiology and health. Despite the overall importance of microbiomes to animal health, they remain understudied in wildlife. Microbiomes function as physical barriers to invading pathogens, and changes in the diversity or composition of microbes within a host ma...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338619/ https://www.ncbi.nlm.nih.gov/pubmed/35908068 http://dx.doi.org/10.1186/s42523-022-00196-7 |
_version_ | 1784760010174103552 |
---|---|
author | Bartlow, Andrew W. Moser, S. Kane Ellis, Jeremy E. Hathcock, Charles D. Fair, Jeanne M. |
author_facet | Bartlow, Andrew W. Moser, S. Kane Ellis, Jeremy E. Hathcock, Charles D. Fair, Jeanne M. |
author_sort | Bartlow, Andrew W. |
collection | PubMed |
description | Microbiomes are essential to a host’s physiology and health. Despite the overall importance of microbiomes to animal health, they remain understudied in wildlife. Microbiomes function as physical barriers to invading pathogens, and changes in the diversity or composition of microbes within a host may disrupt this barrier. In order to use microbiomes in wildlife ecology, knowledge of the natural variation within and among species is essential. We compare the diversity and composition of two avian species that share the same habitat and niche in our study area, the western screech-owl (Megascops kennicottii) and the whiskered screech-owl (M. trichopsis). We used a targeted 16S sequencing method to improve the taxonomic resolution of microbiomes. We found similar measures of alpha diversity between species and sample types (cloacal samples vs. fecal samples). However, there were significant differences in bacterial species richness among nestlings from different nest boxes, and the composition differed between the two bird species and among nestlings from different nest boxes. Western screech-owls had more variation in alpha diversity and composition and had fewer bacterial species in their core microbiome than whiskered screech-owls. Siblings are likely to yield similar findings for microbiomes; thus, sampling nestlings from different nests may be most informative for monitoring population-level changes. |
format | Online Article Text |
id | pubmed-9338619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-93386192022-07-31 Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method Bartlow, Andrew W. Moser, S. Kane Ellis, Jeremy E. Hathcock, Charles D. Fair, Jeanne M. Anim Microbiome Research Microbiomes are essential to a host’s physiology and health. Despite the overall importance of microbiomes to animal health, they remain understudied in wildlife. Microbiomes function as physical barriers to invading pathogens, and changes in the diversity or composition of microbes within a host may disrupt this barrier. In order to use microbiomes in wildlife ecology, knowledge of the natural variation within and among species is essential. We compare the diversity and composition of two avian species that share the same habitat and niche in our study area, the western screech-owl (Megascops kennicottii) and the whiskered screech-owl (M. trichopsis). We used a targeted 16S sequencing method to improve the taxonomic resolution of microbiomes. We found similar measures of alpha diversity between species and sample types (cloacal samples vs. fecal samples). However, there were significant differences in bacterial species richness among nestlings from different nest boxes, and the composition differed between the two bird species and among nestlings from different nest boxes. Western screech-owls had more variation in alpha diversity and composition and had fewer bacterial species in their core microbiome than whiskered screech-owls. Siblings are likely to yield similar findings for microbiomes; thus, sampling nestlings from different nests may be most informative for monitoring population-level changes. BioMed Central 2022-07-30 /pmc/articles/PMC9338619/ /pubmed/35908068 http://dx.doi.org/10.1186/s42523-022-00196-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Bartlow, Andrew W. Moser, S. Kane Ellis, Jeremy E. Hathcock, Charles D. Fair, Jeanne M. Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method |
title | Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method |
title_full | Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method |
title_fullStr | Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method |
title_full_unstemmed | Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method |
title_short | Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method |
title_sort | comparing western (megascops kennicottii) and whiskered (m. trichopsis) screech-owl microbiomes in southern arizona using a novel 16s rrna sequencing method |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338619/ https://www.ncbi.nlm.nih.gov/pubmed/35908068 http://dx.doi.org/10.1186/s42523-022-00196-7 |
work_keys_str_mv | AT bartlowandreww comparingwesternmegascopskennicottiiandwhiskeredmtrichopsisscreechowlmicrobiomesinsouthernarizonausinganovel16srrnasequencingmethod AT moserskane comparingwesternmegascopskennicottiiandwhiskeredmtrichopsisscreechowlmicrobiomesinsouthernarizonausinganovel16srrnasequencingmethod AT ellisjeremye comparingwesternmegascopskennicottiiandwhiskeredmtrichopsisscreechowlmicrobiomesinsouthernarizonausinganovel16srrnasequencingmethod AT hathcockcharlesd comparingwesternmegascopskennicottiiandwhiskeredmtrichopsisscreechowlmicrobiomesinsouthernarizonausinganovel16srrnasequencingmethod AT fairjeannem comparingwesternmegascopskennicottiiandwhiskeredmtrichopsisscreechowlmicrobiomesinsouthernarizonausinganovel16srrnasequencingmethod |