Cargando…

XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps

X-box binding protein 1 (XBP1) is a transcription factor that recognizes the CRE-like element in enhancers of human T-cell leukemia virus and MHC class II gene and induces their transcription. This study was performed to characterize the function of XBP1, which was identified to be a differentially...

Descripción completa

Detalles Bibliográficos
Autores principales: Qu, Xiaopeng, Li, Hongyan, Meng, Lingzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338878/
https://www.ncbi.nlm.nih.gov/pubmed/35915851
http://dx.doi.org/10.1155/2022/3066456
_version_ 1784760061505044480
author Qu, Xiaopeng
Li, Hongyan
Meng, Lingzhao
author_facet Qu, Xiaopeng
Li, Hongyan
Meng, Lingzhao
author_sort Qu, Xiaopeng
collection PubMed
description X-box binding protein 1 (XBP1) is a transcription factor that recognizes the CRE-like element in enhancers of human T-cell leukemia virus and MHC class II gene and induces their transcription. This study was performed to characterize the function of XBP1, which was identified to be a differentially expressed gene via GEO database, in chronic rhinosinusitis (CRS) without nasal polyps (CRSsNP). XBP1 expression was significantly elevated in both CRSsNP patients and mice who were accompanied with mucosal thickening, goblet cell hyperplasia and chemosis, glandular hyperplasia, and dense infiltration of inflammatory cells. Silencing of XBP1 suppressed the development of CRSsNP in mice. Mechanistically, knockdown of XBP1 downregulated the expression of hypoxia-inducible factor 1-alpha (HIF-1a), and overexpression of XBP1 led to the opposite result. Silencing of HIF-1a inhibited β-catenin expression and impaired the Wnt/β-catenin pathway. Further overexpression of HIF-1a in XBP1-silenced CRSsNP mice exacerbated pathological changes in mouse nasal mucosal tissues, promoted inflammation, and activated the Wnt/β-catenin pathway. Taken together, overexpression of XBP1 may be associated with increased expression of HIF-1a and possibly contribute to the Wnt/β-catenin pathway activation and the development of CRSsNP.
format Online
Article
Text
id pubmed-9338878
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-93388782022-07-31 XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps Qu, Xiaopeng Li, Hongyan Meng, Lingzhao Anal Cell Pathol (Amst) Research Article X-box binding protein 1 (XBP1) is a transcription factor that recognizes the CRE-like element in enhancers of human T-cell leukemia virus and MHC class II gene and induces their transcription. This study was performed to characterize the function of XBP1, which was identified to be a differentially expressed gene via GEO database, in chronic rhinosinusitis (CRS) without nasal polyps (CRSsNP). XBP1 expression was significantly elevated in both CRSsNP patients and mice who were accompanied with mucosal thickening, goblet cell hyperplasia and chemosis, glandular hyperplasia, and dense infiltration of inflammatory cells. Silencing of XBP1 suppressed the development of CRSsNP in mice. Mechanistically, knockdown of XBP1 downregulated the expression of hypoxia-inducible factor 1-alpha (HIF-1a), and overexpression of XBP1 led to the opposite result. Silencing of HIF-1a inhibited β-catenin expression and impaired the Wnt/β-catenin pathway. Further overexpression of HIF-1a in XBP1-silenced CRSsNP mice exacerbated pathological changes in mouse nasal mucosal tissues, promoted inflammation, and activated the Wnt/β-catenin pathway. Taken together, overexpression of XBP1 may be associated with increased expression of HIF-1a and possibly contribute to the Wnt/β-catenin pathway activation and the development of CRSsNP. Hindawi 2022-07-23 /pmc/articles/PMC9338878/ /pubmed/35915851 http://dx.doi.org/10.1155/2022/3066456 Text en Copyright © 2022 Xiaopeng Qu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qu, Xiaopeng
Li, Hongyan
Meng, Lingzhao
XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps
title XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps
title_full XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps
title_fullStr XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps
title_full_unstemmed XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps
title_short XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps
title_sort xbp1 regulates the transcription of hif-1a in balb/c mice with chronic rhinosinusitis without polyps
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338878/
https://www.ncbi.nlm.nih.gov/pubmed/35915851
http://dx.doi.org/10.1155/2022/3066456
work_keys_str_mv AT quxiaopeng xbp1regulatesthetranscriptionofhif1ainbalbcmicewithchronicrhinosinusitiswithoutpolyps
AT lihongyan xbp1regulatesthetranscriptionofhif1ainbalbcmicewithchronicrhinosinusitiswithoutpolyps
AT menglingzhao xbp1regulatesthetranscriptionofhif1ainbalbcmicewithchronicrhinosinusitiswithoutpolyps