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Fount, fate, features, and function of renal erythropoietin-producing cells

Renal erythropoietin (Epo)-producing (REP) cells represent a rare and incompletely understood cell type. REP cells are fibroblast-like cells located in close proximity to blood vessels and tubules of the corticomedullary border region. Epo mRNA in REP cells is produced in a pronounced “on–off” mode,...

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Autores principales: Dahl, Sophie L., Bapst, Andreas M., Khodo, Stellor Nlandu, Scholz, Carsten C., Wenger, Roland H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338912/
https://www.ncbi.nlm.nih.gov/pubmed/35750861
http://dx.doi.org/10.1007/s00424-022-02714-7
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author Dahl, Sophie L.
Bapst, Andreas M.
Khodo, Stellor Nlandu
Scholz, Carsten C.
Wenger, Roland H.
author_facet Dahl, Sophie L.
Bapst, Andreas M.
Khodo, Stellor Nlandu
Scholz, Carsten C.
Wenger, Roland H.
author_sort Dahl, Sophie L.
collection PubMed
description Renal erythropoietin (Epo)-producing (REP) cells represent a rare and incompletely understood cell type. REP cells are fibroblast-like cells located in close proximity to blood vessels and tubules of the corticomedullary border region. Epo mRNA in REP cells is produced in a pronounced “on–off” mode, showing transient transcriptional bursts upon exposure to hypoxia. In contrast to “ordinary” fibroblasts, REP cells do not proliferate ex vivo, cease to produce Epo, and lose their identity following immortalization and prolonged in vitro culture, consistent with the loss of Epo production following REP cell proliferation during tissue remodelling in chronic kidney disease. Because Epo protein is usually not detectable in kidney tissue, and Epo mRNA is only transiently induced under hypoxic conditions, transgenic mouse models have been developed to permanently label REP cell precursors, active Epo producers, and inactive descendants. Future single-cell analyses of the renal stromal compartment will identify novel characteristic markers of tagged REP cells, which will provide novel insights into the regulation of Epo expression in this unique cell type.
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spelling pubmed-93389122022-08-01 Fount, fate, features, and function of renal erythropoietin-producing cells Dahl, Sophie L. Bapst, Andreas M. Khodo, Stellor Nlandu Scholz, Carsten C. Wenger, Roland H. Pflugers Arch Invited Review Renal erythropoietin (Epo)-producing (REP) cells represent a rare and incompletely understood cell type. REP cells are fibroblast-like cells located in close proximity to blood vessels and tubules of the corticomedullary border region. Epo mRNA in REP cells is produced in a pronounced “on–off” mode, showing transient transcriptional bursts upon exposure to hypoxia. In contrast to “ordinary” fibroblasts, REP cells do not proliferate ex vivo, cease to produce Epo, and lose their identity following immortalization and prolonged in vitro culture, consistent with the loss of Epo production following REP cell proliferation during tissue remodelling in chronic kidney disease. Because Epo protein is usually not detectable in kidney tissue, and Epo mRNA is only transiently induced under hypoxic conditions, transgenic mouse models have been developed to permanently label REP cell precursors, active Epo producers, and inactive descendants. Future single-cell analyses of the renal stromal compartment will identify novel characteristic markers of tagged REP cells, which will provide novel insights into the regulation of Epo expression in this unique cell type. Springer Berlin Heidelberg 2022-06-24 2022 /pmc/articles/PMC9338912/ /pubmed/35750861 http://dx.doi.org/10.1007/s00424-022-02714-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Invited Review
Dahl, Sophie L.
Bapst, Andreas M.
Khodo, Stellor Nlandu
Scholz, Carsten C.
Wenger, Roland H.
Fount, fate, features, and function of renal erythropoietin-producing cells
title Fount, fate, features, and function of renal erythropoietin-producing cells
title_full Fount, fate, features, and function of renal erythropoietin-producing cells
title_fullStr Fount, fate, features, and function of renal erythropoietin-producing cells
title_full_unstemmed Fount, fate, features, and function of renal erythropoietin-producing cells
title_short Fount, fate, features, and function of renal erythropoietin-producing cells
title_sort fount, fate, features, and function of renal erythropoietin-producing cells
topic Invited Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338912/
https://www.ncbi.nlm.nih.gov/pubmed/35750861
http://dx.doi.org/10.1007/s00424-022-02714-7
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