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Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338969/ https://www.ncbi.nlm.nih.gov/pubmed/35907922 http://dx.doi.org/10.1038/s41597-022-01569-y |
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author | David, Caroline K. Sugimura, Yae K. Kallurkar, Prajkta S. Picardo, Maria Cristina D. Saha, Margaret S. Conradi Smith, Gregory D. Del Negro, Christopher A. |
author_facet | David, Caroline K. Sugimura, Yae K. Kallurkar, Prajkta S. Picardo, Maria Cristina D. Saha, Margaret S. Conradi Smith, Gregory D. Del Negro, Christopher A. |
author_sort | David, Caroline K. |
collection | PubMed |
description | Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements. |
format | Online Article Text |
id | pubmed-9338969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93389692022-08-01 Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice David, Caroline K. Sugimura, Yae K. Kallurkar, Prajkta S. Picardo, Maria Cristina D. Saha, Margaret S. Conradi Smith, Gregory D. Del Negro, Christopher A. Sci Data Data Descriptor Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements. Nature Publishing Group UK 2022-07-30 /pmc/articles/PMC9338969/ /pubmed/35907922 http://dx.doi.org/10.1038/s41597-022-01569-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Data Descriptor David, Caroline K. Sugimura, Yae K. Kallurkar, Prajkta S. Picardo, Maria Cristina D. Saha, Margaret S. Conradi Smith, Gregory D. Del Negro, Christopher A. Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice |
title | Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice |
title_full | Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice |
title_fullStr | Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice |
title_full_unstemmed | Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice |
title_short | Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice |
title_sort | single cell transcriptome sequencing of inspiratory neurons of the prebötzinger complex in neonatal mice |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338969/ https://www.ncbi.nlm.nih.gov/pubmed/35907922 http://dx.doi.org/10.1038/s41597-022-01569-y |
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