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Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice

Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in...

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Autores principales: David, Caroline K., Sugimura, Yae K., Kallurkar, Prajkta S., Picardo, Maria Cristina D., Saha, Margaret S., Conradi Smith, Gregory D., Del Negro, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338969/
https://www.ncbi.nlm.nih.gov/pubmed/35907922
http://dx.doi.org/10.1038/s41597-022-01569-y
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author David, Caroline K.
Sugimura, Yae K.
Kallurkar, Prajkta S.
Picardo, Maria Cristina D.
Saha, Margaret S.
Conradi Smith, Gregory D.
Del Negro, Christopher A.
author_facet David, Caroline K.
Sugimura, Yae K.
Kallurkar, Prajkta S.
Picardo, Maria Cristina D.
Saha, Margaret S.
Conradi Smith, Gregory D.
Del Negro, Christopher A.
author_sort David, Caroline K.
collection PubMed
description Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements.
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spelling pubmed-93389692022-08-01 Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice David, Caroline K. Sugimura, Yae K. Kallurkar, Prajkta S. Picardo, Maria Cristina D. Saha, Margaret S. Conradi Smith, Gregory D. Del Negro, Christopher A. Sci Data Data Descriptor Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements. Nature Publishing Group UK 2022-07-30 /pmc/articles/PMC9338969/ /pubmed/35907922 http://dx.doi.org/10.1038/s41597-022-01569-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Data Descriptor
David, Caroline K.
Sugimura, Yae K.
Kallurkar, Prajkta S.
Picardo, Maria Cristina D.
Saha, Margaret S.
Conradi Smith, Gregory D.
Del Negro, Christopher A.
Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
title Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
title_full Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
title_fullStr Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
title_full_unstemmed Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
title_short Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
title_sort single cell transcriptome sequencing of inspiratory neurons of the prebötzinger complex in neonatal mice
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338969/
https://www.ncbi.nlm.nih.gov/pubmed/35907922
http://dx.doi.org/10.1038/s41597-022-01569-y
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