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Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae

Progression through meiosis is associated with significant reorganization of chromosome structure, regulated in part by changes in histones and chromatin. Prior studies observed defects in meiotic progression in yeast strains lacking the linker histone H1 or variant histone H2A.Z. To further define...

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Autores principales: Chigweshe, Lorencia, MacQueen, Amy J, Holmes, Scott G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339299/
https://www.ncbi.nlm.nih.gov/pubmed/35608312
http://dx.doi.org/10.1093/g3journal/jkac128
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author Chigweshe, Lorencia
MacQueen, Amy J
Holmes, Scott G
author_facet Chigweshe, Lorencia
MacQueen, Amy J
Holmes, Scott G
author_sort Chigweshe, Lorencia
collection PubMed
description Progression through meiosis is associated with significant reorganization of chromosome structure, regulated in part by changes in histones and chromatin. Prior studies observed defects in meiotic progression in yeast strains lacking the linker histone H1 or variant histone H2A.Z. To further define the contributions of these chromatin factors, we have conducted genetic and cytological analysis of cells undergoing meiosis in the absence of H1 and H2A.Z. We find that a spore viability defect observed in strains lacking H2A.Z can be partially suppressed if cells also lack histone H1, while the combined loss of both H1 and H2A.Z is associated with elevated gene conversion events. Cytological analysis of Red1 and Rec8 staining patterns indicates that a subset of cells lacking H2A.Z fail to assemble a proper chromosome axis, and the staining pattern of the synaptonemal complex protein Zip1 in htz1Δ/htz1Δ cells mimics that of cells deficient for Rec8-dependent meiotic cohesion. Our results suggest a role for H2A.Z in the establishment or maintenance of the meiotic chromosome axis, possibly by promoting the efficient chromosome cohesion.
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spelling pubmed-93392992022-08-01 Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae Chigweshe, Lorencia MacQueen, Amy J Holmes, Scott G G3 (Bethesda) Investigation Progression through meiosis is associated with significant reorganization of chromosome structure, regulated in part by changes in histones and chromatin. Prior studies observed defects in meiotic progression in yeast strains lacking the linker histone H1 or variant histone H2A.Z. To further define the contributions of these chromatin factors, we have conducted genetic and cytological analysis of cells undergoing meiosis in the absence of H1 and H2A.Z. We find that a spore viability defect observed in strains lacking H2A.Z can be partially suppressed if cells also lack histone H1, while the combined loss of both H1 and H2A.Z is associated with elevated gene conversion events. Cytological analysis of Red1 and Rec8 staining patterns indicates that a subset of cells lacking H2A.Z fail to assemble a proper chromosome axis, and the staining pattern of the synaptonemal complex protein Zip1 in htz1Δ/htz1Δ cells mimics that of cells deficient for Rec8-dependent meiotic cohesion. Our results suggest a role for H2A.Z in the establishment or maintenance of the meiotic chromosome axis, possibly by promoting the efficient chromosome cohesion. Oxford University Press 2022-05-24 /pmc/articles/PMC9339299/ /pubmed/35608312 http://dx.doi.org/10.1093/g3journal/jkac128 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Chigweshe, Lorencia
MacQueen, Amy J
Holmes, Scott G
Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae
title Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae
title_full Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae
title_fullStr Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae
title_full_unstemmed Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae
title_short Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae
title_sort histone variant h2a.z promotes meiotic chromosome axis organization in saccharomyces cerevisiae
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339299/
https://www.ncbi.nlm.nih.gov/pubmed/35608312
http://dx.doi.org/10.1093/g3journal/jkac128
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